Differential expression of motilin receptors on the endothelium of dog gastrointestinal arteries and motilin-induced motilin receptor dependent relaxation of corresponding arteries

Yang, Lifang; Li, Hongyu; Jin, Ying; He, Yu; Li, Mei; Jin, Chunxiang

doi:10.1016/j.peptides.2021.170574

Cited by 2 publications

(8 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent studies have shown that MLNRs are present on the ECs of gastrointestinal arteries (Yang et al, 2021), and endothelial MLNR is the molecular basis for the regulation gastric blood flow by motilin in dogs (Jin et al, 2002). The present study identified endothelial MLNR signaling pathways that induce VSM relaxation in the LGA of dogs (Figure 11).…”

Section: Discussionmentioning

confidence: 51%

“…In the mesenteric arteries of dogs, [Leu 13 ]motilin-induced vasorelaxation was inhibited by Nω-nitro-L-arginine [10 −4 M; inhibitor of nitric oxide synthase (NOS)], which confirmed the participation of endothelial NO, whereas a high dose of GM-109 (10 −4 M; a MLNR antagonist) only slightly decreased the relaxation, which excluded the role of MLNR (Iwai et al, 1998). However, the fact that GM-109 could inhibit motilininduced relaxation of dog LGA both in vivo and in vitro (Jin et al, 2002;Yang et al, 2021) and that motilin induced endothelium-dependent relaxation of dog gastrointestinal arteries (Yang et al, 2021) suggests the irreplaceable roles of both endothelial MLNR and relaxation mediators.…”

Section: Introductionmentioning

confidence: 94%

“…Motilin induces gastric phase III of the migrating motor complex (MMC III) in fasting humans and dogs (Lee et al, 1983;Ogawa et al, 2011;Deloose et al, 2015;Kitazawa and Kaiya, 2019), and also simultaneously induces a sustained increase in blood flow (≤ 240% resting blood flow) of the left gastric artery (LGA; Jin et al, 2002). The expression of MLNR has recently been found on the membrane of endothelial cells (ECs) in canine gastrointestinal arteries (Yang et al, 2021). However, the signal transduction pathways by which motilin induces vascular smooth muscle (VSM) relaxation is unclear.…”

Section: Introductionmentioning

confidence: 99%

“…Yet, the physiological function of this expression is not fully understood. However, endothelial MLNR is the molecular basis to allow motilin to regulate gastric artery blood flow in dogs under physiological conditions (Jin et al, 2002;Yang et al, 2021). Thus, it is of physiological and pathophysiological significance to study its signal transduction pathway.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the MLNR-coupled G protein pathway in ECs of the LGA is properly consistent with that in gastrointestinal SMCs. Motilin-induced LGA relaxation involves cooperation between ECs and SMCs (Yang et al, 2021). Endothelium-dependent relaxation is achieved through a combination of endotheliumderived prostacyclin (PGI 2 ), nitric oxide (NO), and endotheliumderived hyperpolarizing factor (EDHF) by different mechanisms (Kukovetz et al, 1979;Carvajal et al, 2000;Félétou, 2016).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Roles of Endothelial Motilin Receptor and Its Signal Transduction Pathway in Motilin-Induced Left Gastric Artery Relaxation in Dogs

Yang

Jin

et al. 2021

Front. Physiol.

Self Cite

View full text Add to dashboard Cite

Background: Motilin increases left gastric artery (LGA) blood flow in dogs via the endothelial motilin receptor (MLNR). This article investigates the signaling pathways of endothelial MLNR.Methods: Motilin-induced relaxation of LGA rings was assessed using wire myography. Nitric oxide (NO), and cyclic guanosine monophosphate (cGMP) levels were measured using an NO assay kit and cGMP ELISA kit, respectively.Results: Motilin concentration-dependently (EC50=9.1±1.2×10−8M) relaxed LGA rings precontracted with U46619 (thromboxane A2 receptor agonist). GM-109 (MLNR antagonist) significantly inhibited motilin-induced LGA relaxation and the production of NO and cGMP. N-ethylmaleimide (NEM; G-protein antagonist), U73122 [phospholipase C (PLC) inhibitor], and 2-aminoethyl diphenylborinate [2-APB; inositol trisphosphate (IP3) blocker] partially or completely blocked vasorelaxation. In contrast, chelerythrine [protein kinase C (PKC) inhibitor] and H89 [protein kinase A (PKA) inhibitor] had no such effect. Low-calcium or calcium-free Krebs solutions also reduced vasorelaxation. N-nitro-L-arginine methyl ester [L-NAME; nitric oxide synthase (NOS) inhibitor] and ODQ [soluble guanylyl cyclase (sGC) inhibitor] completely abolished vasodilation and synthesis of NO and cGMP. Indomethacin (cyclooxygenase inhibitor), 18α-glycyrrhetinic acid [18α-GA; myoendothelial gap junction (MEGJ) inhibitor], and K+ channel inhibition through high K+ concentrations or tetraethylammonium (TEA-Cl; KCa channel blocker) partially decreased vasorelaxation, whereas glibenclamide (KATP channel blocker) had no such effect.Conclusion: The current study suggests that motilin-induced LGA relaxation is dependent on endothelial MLNR through the G protein-PLC-IP3 pathway and Ca2+ influx. The NOS-NO-sGC-cGMP pathway, prostacyclin, MEGJ, and K+ channels (especially KCa) are involved in endothelial-dependent relaxation of vascular smooth muscle (VSM) cells.

show abstract

Section: Discussionmentioning

confidence: 51%

Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Roles of Endothelial Motilin Receptor and Its Signal Transduction Pathway in Motilin-Induced Left Gastric Artery Relaxation in Dogs

Yang

Jin

et al. 2021

Front. Physiol.

Self Cite

View full text Add to dashboard Cite

show abstract

Motilin, a Novel Orexigenic Factor, Involved in Feeding Regulation in Yangtze Sturgeon (Acipenser dabryanus)

Tang,

Li,

et al. 2024

Biomolecules

View full text Add to dashboard Cite

Motilin is a gastrointestinal hormone that is mainly produced in the duodenum of mammals, and it is responsible for regulating appetite. However, the role and expression of motilin are poorly understood during starvation and the weaning stage, which is of great importance in the seeding cultivation of fish. In this study, the sequences of Yangtze sturgeon (Acipenser dabryanus Motilin (AdMotilin)) motilin receptor (AdMotilinR) were cloned and characterized. The results of tissue expression showed that by contrast with mammals, AdMotilin mRNA was richly expressed in the brain, whereas AdMotilinR was highly expressed in the stomach, duodenum, and brain. Weaning from a natural diet of T. Limnodrilus to commercial feed significantly promoted the expression of AdMotilin in the brain during the period from day 1 to day 10, and after re-feeding with T. Limnodrilus the change in expression of AdMotilin was partially reversed. Similarly, it was revealed that fasting increased the expression of AdMotilin in the brain (3 h, 6 h) and duodenum (3 h), and the expression of AdMotilinR in the brain (1 h) in a time-dependent manner. Furthermore, it was observed that peripheral injection of motilin-NH2 increased food intake and the filling index of the digestive tract in the Yangtze sturgeon, which was accompanied by the changes of AdMotilinR and appetite factors expression in the brain (POMC, CART, AGRP, NPY and CCK) and stomach (CCK). These results indicate that motilin acts as an indicator of nutritional status, and also serves as a novel orexigenic factor that stimulates food intake in Acipenser dabryanus. This study lays a strong foundation for the application of motilin as a biomarker in the estimation of hunger in juvenile Acipenser dabryanu during the weaning phase, and enhances the understanding of the role of motilin as a novel regulator of feeding in fish.

show abstract

Differential expression of motilin receptors on the endothelium of dog gastrointestinal arteries and motilin-induced motilin receptor dependent relaxation of corresponding arteries

Cited by 2 publications

References 39 publications

Roles of Endothelial Motilin Receptor and Its Signal Transduction Pathway in Motilin-Induced Left Gastric Artery Relaxation in Dogs

Roles of Endothelial Motilin Receptor and Its Signal Transduction Pathway in Motilin-Induced Left Gastric Artery Relaxation in Dogs

Motilin, a Novel Orexigenic Factor, Involved in Feeding Regulation in Yangtze Sturgeon (Acipenser dabryanus)

Contact Info

Product

Resources

About