Differential expression of nickel toxicity on Allium cepa L. seeds and seedlings

Manna, Indrani; Bandyopadhyay, Maumita

doi:10.1007/s13762-022-04625-7

Cited by 2 publications

(1 citation statement)

References 110 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Excess copper causes double-stranded breaks, DNA oxidation, and an overall reduction in DNA content [85][86][87]. Excess nickel has also been shown to elicit DNA damage and reduce DNA content, although the specific mechanisms are not well documented in plants [88,89]. The high proportion of genes associated with nuclease activity indicates different levels of DNA repair mechanisms, which may be used to mitigate the oxidative stress on DNA induced by heavy metals [90].…”

Section: Deg Analysis Reveals Different Patterns Of Gene Expression B...mentioning

confidence: 99%

Decrypting Molecular Mechanisms Involved in Counteracting Copper and Nickel Toxicity in Jack Pine (Pinus banksiana) Based on Transcriptomic Analysis

Moy,

Nkongolo

2024

Plants

View full text Add to dashboard Cite

The remediation of copper and nickel-afflicted sites is challenged by the different physiological effects imposed by each metal on a given plant system. Pinus banksiana is resilient against copper and nickel, providing an opportunity to build a valuable resource to investigate the responding gene expression toward each metal. The objectives of this study were to (1) extend the analysis of the Pinus banksiana transcriptome exposed to nickel and copper, (2) assess the differential gene expression in nickel-resistant compared to copper-resistant genotypes, and (3) identify mechanisms specific to each metal. The Illumina platform was used to sequence RNA that was extracted from seedlings treated with each of the metals. There were 449 differentially expressed genes (DEGs) between copper-resistant genotypes (RGs) and nickel-resistant genotypes (RGs) at a high stringency cut-off, indicating a distinct pattern of gene expression toward each metal. For biological processes, 19.8% of DEGs were associated with the DNA metabolic process, followed by the response to stress (13.15%) and the response to chemicals (8.59%). For metabolic function, 27.9% of DEGs were associated with nuclease activity, followed by nucleotide binding (27.64%) and kinase activity (10.16%). Overall, 21.49% of DEGs were localized to the plasma membrane, followed by the cytosol (16.26%) and chloroplast (12.43%). Annotation of the top upregulated genes in copper RG compared to nickel RG identified genes and mechanisms that were specific to copper and not to nickel. NtPDR, AtHIPP10, and YSL1 were identified as genes associated with copper resistance. Various genes related to cell wall metabolism were identified, and they included genes encoding for HCT, CslE6, MPG, and polygalacturonase. Annotation of the top downregulated genes in copper RG compared to nickel RG revealed genes and mechanisms that were specific to nickel and not copper. Various regulatory and signaling-related genes associated with the stress response were identified. They included UGT, TIFY, ACC, dirigent protein, peroxidase, and glyoxyalase I. Additional research is needed to determine the specific functions of signaling and stress response mechanisms in nickel-resistant plants.

show abstract

Section: Deg Analysis Reveals Different Patterns Of Gene Expression B...mentioning

confidence: 99%

Decrypting Molecular Mechanisms Involved in Counteracting Copper and Nickel Toxicity in Jack Pine (Pinus banksiana) Based on Transcriptomic Analysis

Moy,

Nkongolo

2024

Plants

View full text Add to dashboard Cite

show abstract

DNA fragmentation, chromosomal aberrations, and multi-toxic effects induced by nickel and the modulation of Ni-induced damage by pomegranate seed extract in Allium cepa L

Yılmaz,

Kalefetoğlu Macar,

Macar

et al. 2023

Environ Sci Pollut Res

View full text Add to dashboard Cite

Differential expression of nickel toxicity on Allium cepa L. seeds and seedlings

Cited by 2 publications

References 110 publications

Decrypting Molecular Mechanisms Involved in Counteracting Copper and Nickel Toxicity in Jack Pine (Pinus banksiana) Based on Transcriptomic Analysis

Decrypting Molecular Mechanisms Involved in Counteracting Copper and Nickel Toxicity in Jack Pine (Pinus banksiana) Based on Transcriptomic Analysis

DNA fragmentation, chromosomal aberrations, and multi-toxic effects induced by nickel and the modulation of Ni-induced damage by pomegranate seed extract in Allium cepa L

Contact Info

Product

Resources

About