Differential Expression of nm23 H-1 Protein in Conjunctival Melanoma and Potential Precursor Lesions

Seregard, Stefan; Oskarsson, Margareta; Spångberg, Berit

doi:10.1006/exer.1999.0746

Cited by 2 publications

(2 citation statements)

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“…The tentative association of nm23-H1 immunoexpression levels with cause-specific survival in this study was statistically significant (P < 0.05). Our results, however, differed from previous reports that did not find a correlation between the survival rate and the immunostaining levels of nm23-H1, likely because most of these studies graded the immunostaining levels irrespective of immunostaining intensity [21][22][23].…”

Section: Discussioncontrasting

confidence: 85%

“…Consequently, Greco et al [22] showed that low or absent nm23 protein expression appears to be associated with predictive parameters of an adverse prognosis in UM. On the other hand, in a study of 46 primary conjunctival melanomas, the authors did not find a statistically significant relationship between nm23 immunostaining and patient survival rate, or between primary lesions and recurrent tumors [23]. Later on, Bardak et al [24] studied immunostaining levels of nm23 in 34 consecutive cases of choroidal melanomas to determine its relationship with clinical and histopathological characteristics of the aforementioned tumors.…”

Section: Discussionmentioning

confidence: 96%

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Expression of nm23-H1 in uveal melanoma

Bakalian

Marshall²,

Faingold³

et al. 2007

Melanoma Research

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Uveal melanoma (UM) is the most common malignant intraocular tumor in adults. Despite the high accuracy of clinical diagnosis and advances in local treatment, more than 50% of UM patients develop metastasis within 10 years of initial diagnosis. NM23 is one of the human metastasis suppressor genes. Reduced nm23-H1 expression is correlated with high metastatic potential in many different cancers. The purpose of this study is to investigate the expression of nm23-H1 in UM and its potential value as a prognostic marker. Immunostaining of nm23-H1 was verified in five human UM cell lines with different metastatic potentials. The expression level of nm23-H1 mRNA was evaluated with one-step quantitative real-time PCR. The invasion ability of the cell lines was assessed before and after silencing nm23-H1 with small interference RNA. Thirty-two cases of paraffin-embedded specimens of human UM were immunostained with nm23-H1 monoclonal antibody. The immunostaining was evaluated in a semiquantitative fashion based on extent and intensity. The real-time PCR results of five human UM cell lines showed that expression of nm23-H1 was higher in cell lines with low metastatic potential compared with those with high metastatic potential (P<0.05). The invasive ability of the UM cell lines increased after silencing nm23-H1 expression with small interference RNA (P<0.05). The immunostaining of nm23-H1 was cytoplasmic in all cell lines and UM patients samples. The increased immunostaining intensity of nm23-H1 in patients' samples was associated with better survival rate (Kaplan-Meier test P=0.0097). The expression of nm23-H1 was not correlated with other prognostic factors. It can be concluded that nm23-H1 may be a prognostic marker to predict the survival rate of UM patients and it has the potential to identify high-risk patients.

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Section: Discussioncontrasting

confidence: 85%