Differential expression of nuclear matrix proteins during the differentiation of human neuroblastoma SK‐N‐SH cells induced by retinoic acid

Liang, Ying; Li, Qifu; Zhang, Xiuyan; Shi, Shengqing; Jing, Guangjun

doi:10.1002/jcb.22052

Cited by 8 publications

(5 citation statements)

References 30 publications

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“…Only a few short neurites were observable in control samples in comparison with cells treated with 1 µM ATRA (Figure 2b). Here, as expected from previous studies [49,50], a notable reduction in the total number of cells and a prevalence of cells with a globose cell body with numerous neurites was evident. logical differentiation, 10 randomly selected brightfield images were captured in at least three wells per condition after 5 days of incubation.…”

Section: Morphological Analysis and Cell Growth Assessmentsupporting

confidence: 89%

Effects of Anionic Liposome Delivery of All–Trans–Retinoic Acid on Neuroblastoma Cell Differentiation

Minò,

Lopez,

Barbaro

et al. 2024

Biomimetics

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All–trans–retinoic acid (ATRA) has long been known to affect cell growth and differentiation. To improve ATRA’s therapeutic efficacy and pharmacodynamics, several delivery systems have been used. In this study, free ATRA and anionic–liposome–encapsulated ATRA were compared for their effects on SK–N–SH human neuroblastoma cell growth and differentiation. Anionic liposomes made of L–α–phosphatidylcholine (PC) and L–α–phosphatidic acid (PA), empty (PC–PA) and loaded with ATRA (PC–PA–ATRA), were characterized by dynamic light scattering (DLS) and electrophoretic mobility measurements, and drug entrapment efficiency (EE%) was measured to evaluate the applicability of the new colloidal formulation. The results of brightfield microscopy and cell growth curves indicated that ATRA, whether free or encapsulated, reduced growth and induced differentiation, resulting in SK–N–SH cells changing from epithelioid to neuronal–like morphologies, and producing a significant increase in neurite growth. To further characterize the neuro-differentiation of SK–N–SH cells, the expression of βIII–Tubulin and synaptophysin and mitochondria localization were analyzed via immunofluorescence. Increased expression of neuronal markers and a peculiar localization of mitochondria in the neuritic extensions were apparent both in ATRA– and PC–PA–ATRA–differentiated cells. As a whole, our results strongly indicate that ATRA treatment, by any means, can induce the differentiation of parent SK–N–SH, and they highlight that its encapsulation in anionic liposomes increases its differentiation ability in terms of the percentage of neurite–bearing cells. Interestingly, our data also suggest an unexpected differentiation capability of anionic liposomes per se. This work highlights the importance of developing and carefully testing novel delivery nanocarriers, which are a necessary first “step” in the development of new therapeutic settings.

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Section: Morphological Analysis and Cell Growth Assessmentsupporting

confidence: 89%

Effects of Anionic Liposome Delivery of All–Trans–Retinoic Acid on Neuroblastoma Cell Differentiation

Minò,

Lopez,

Barbaro

et al. 2024

Biomimetics

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“…In our previous study, we found NPM was a nuclear matrix protein and its expression was downregulated in the induced differentiation of human osteosarcoma, human neuroblastoma tumors and human liver cancer (6)(7)(8), indicating NPM functioned in regulating the differentiation of tumor cells (9). Liver cancer is the most common malignant gastrointestinal cancer and the screening for functional proteins related to liver tumor cell differentiation will contribute to the elucidation of the mechanism of liver cancer development and its early diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Regulatory role of nucleophosmin during the differentiation of human liver cancer cells

Liu

et al. 2014

International Journal of Oncology

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Abstract. Nucleophosmin (NPM, also known as B23), mainly localized in the nucleolus, has been reported to be overexpressed in many types of human cancer, including colon, ovarian, prostate and gastric cancer. NPM was identified while screening the differential nuclear matrix proteins during HMBA-induced differentiation of human liver cancer cells. We investigated the aberrant expression and subcellular localization of NPM in clinical liver cancer tissues and a cell line with the aim of providing more evidence for revealing the roles of NPM on regulating liver cancer cell proliferation and differentiation. In addition, we studied the potential interaction between NPM and several important proteins. Our results revealed that NPM protein was overexpressed in cancer cells, which was in accordance with the overexpressed mRNA in cancer tissues compared to the corresponding noncancer tissues. We also found a decrease of NPM in protein and mRNA levels upon treatment with the differentiation reagent HMBA. We focused on the aberrant localization of NPM. Immunochemistry and immunofluorescence revealed aberrant cytoplasmic and nucleoplasm localization of NPM in liver cancer tissues and its colocalization with c-Myc, c-Fos, P53 and Rb in the SMMC-7721 cell line. The interactions between NPM and the above proteins were confirmed by GST pull-down assay and co-immunoprecipitation assay. These findings indicate that NPM plays a regulatory role in liver cancer, which deserves in-depth investigation. IntroductionNucleophosmin (NPM) is a ubiquitous nucleolar phosphoprotein with multifunction. NPM has been shown to associate with the assembly and transport of ribosome, the duplication of centrosome and DNA damage response (1,2). NPM regulates the nucleolus activities and cell proliferation, indicating its crucial roles in cell growth. However, the roles of NPM in cell differentiation and the mechanisms of its transportation and relocation are still largely unknown. Recent data suggest NPM is tightly correlated with the development of a tumor (1), its expression level is enhanced in various cancers, such as bladder cancer and prostate cancer (3-5). As a potential proto-oncogene, NPM can affect cell growth through multiple pathways (1). Nevertheless, how NPM affects the reversal of malignant type of tumor cells is still unknown.In our previous study, we found NPM was a nuclear matrix protein and its expression was downregulated in the induced differentiation of human osteosarcoma, human neuroblastoma tumors and human liver cancer (6-8), indicating NPM functioned in regulating the differentiation of tumor cells (9). Liver cancer is the most common malignant gastrointestinal cancer and the screening for functional proteins related to liver tumor cell differentiation will contribute to the elucidation of the mechanism of liver cancer development and its early diagnosis. Hereby, based on the induced effect of hexamethylene bisacetamide (HMBA) on the differentiation of human liver cancer cells, we extended our study to investigate the loca...

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“…Our previous study has found an aberrant expression of hnRNP A2/B1 in nuclear matrix‐intermediate filament system during the induced‐differentiation of human osteosarcoma MG‐63 cells and human neuroblastoma SK‐N‐SH cells, suggesting that hnRNP A2/B1 is closely related with the proliferation and differentiation of human neuroblastoma SK‐N‐SH cells [Zhao et al, 2008; Liang et al, 2009]. Therefore in this study, retinoic acid (RA), a classical inducer [Liang et al, 2009; Tonge and Andrews, 2010], was used to induce human neruoblastoma SK‐N‐SH cells into differentiation. We investigated the distribution and expression of hnRNP A2/B1 in the nuclear matrix, and primarily explored the relationship of hnRNP A2/B1 with related oncogenes and tumor suppressor genes.…”

mentioning

confidence: 99%

The localization of hnRNP A2/B1 in nuclear matrix and the aberrant expression during the RA‐induced differentiation of human neuroblastoma SK‐N‐SH cells

Liang¹,

Shi²,

Li³

et al. 2011

J of Cellular Biochemistry

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Heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 is involved in the synthesis of RNA. Its expression is up-regulated in many tumor cell lines. In this study, we investigated the distribution of hnRNP A2/B1 in the nuclear matrix, including its co-localization with expression products of related genes. Results from 2-DE PAGE and MS showed that hnRNP A2/B1 is involved with components of nuclear matrix proteins of SK-N-SH cells, and that its expression level is down-regulated after retinoic acid (RA) treatment. Protein immunoblotting results further confirm the existence of hnRNP A2/B1 in the nuclear matrix, as well as its down-regulation after RA treatment. Immunofluorescence microscopy observation showed that hnRNP A2/B1 localized in nuclear matrix of SK-N-SH cells and its distribution regions were altered after RA treatment. Laser scanning confocal microscopy observation showed that hnRNP A2/B1 co-localized with c-Myc, c-Fos, P53, and Rb in SK-N-SH cells. The co-localized region was altered as a result of RA treatment. Our data proved that hnRNP A2/B1 is a nuclear matrix protein and can be up-regulated in human neuroblastoma. The expression and distribution of hnRNP A2/B1 can affect the differentiation of SK-N-SH cells, as well as its co-localization with related oncogenes and tumor suppressor genes.

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Differential expression of nuclear matrix proteins during the differentiation of human neuroblastoma SK‐N‐SH cells induced by retinoic acid

Cited by 8 publications

References 30 publications

Effects of Anionic Liposome Delivery of All–Trans–Retinoic Acid on Neuroblastoma Cell Differentiation

Effects of Anionic Liposome Delivery of All–Trans–Retinoic Acid on Neuroblastoma Cell Differentiation

Regulatory role of nucleophosmin during the differentiation of human liver cancer cells

The localization of hnRNP A2/B1 in nuclear matrix and the aberrant expression during the RA‐induced differentiation of human neuroblastoma SK‐N‐SH cells

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