Differential expression of p63 isoforms in normal skin and hyperproliferative conditions

Kim, So Young; Cho, Hyun Joo; Kim, Do Kyung; Choi, Hae Ryung; Kwon, Sun Bang; Na, Jung Im; Jeon, Hye Chan; Huh, Chang Hoon; Youn, Sang Woong; Cho, Kwang Hyun; Park, Kyoung Chan

doi:10.1111/j.1600-0560.2008.01167.x

Cited by 4 publications

(4 citation statements)

References 11 publications

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“…As already mentioned, p63 is known as a putative stem cell marker of skin. In normal skin, expression of p63 was noticeable in the nuclei of cells in the basal and suprabasal layers of the epidermis . Our results revealed that GHK increased the number of p63 positive cells (Figure ).…”

Section: Discussionsupporting

confidence: 57%

Stem cell recovering effect of copper‐free GHK in skin

Choi

Kang

Ryoo

et al. 2012

Journal of Peptide Science

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The peptide Gly-His-Lys (GHK) is a naturally occurring copper(II)-chelating motifs in human serum and cerebrospinal fluid. In industry, GHK (with or without copper) is used to make hair and skin care products. Copper-GHK plays a physiological role in the process of wound healing and tissue repair by stimulating collagen synthesis in fibroblasts. We also reported that copper-GHK promotes the survival of basal stem cells in the skin. However, the effects of copper-free GHK (GHK) have not been investigated well. In this study, the effects of GHK were studied using cultured normal human keratinocytes and skin equivalent (SE) models. In monolayer cultured keratinocytes, GHK increased the proliferation of keratinocytes. When GHK was added during the culture of SE models, the basal cells became more cuboidal than control model. In addition, there was linear and intense staining of α6 and β1 integrin along the basement membrane. The number of p63 and proliferating cell nuclear antigen positive cells was also significantly increased in GHK-treated SEs than in control SEs. Western blot and slide culture experiment showed that GHK increased the expression of integrin by keratinocytes. All these results showed that GHK increased the stemness and proliferative potential of epidermal basal cells, which is associated with increased expression of integrin. In conclusion, copper-free GHK showed similar effects with copper-GHK. Thus, it can be said that copper-free GHK can be used in industry to obtain the effects of copper-GHK in vivo. Further study is necessary to explore the relationship between copper-free GHK and copper-GHK.

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Section: Discussionsupporting

confidence: 57%

Stem cell recovering effect of copper‐free GHK in skin

Choi

Kang

Ryoo

et al. 2012

Journal of Peptide Science

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“…The normal Ki-67 and p63 protein expression in the lesional skin of our patient is consistent with previous observations showing that the p63 protein is expressed in the skin despite the presence of a mutation [27][28][29]. Molecular alterations probably result in reduced transcriptional activity of the impaired p63 on several gene promoters [30][31][32][33][34][35].…”

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confidence: 78%

Ectrodactyly-ectodermal dysplasia-clefting syndrome with unusual cutaneous vitiligoid and psoriasiform lesions due to a novel single point TP63 gene mutation

Wawrzycki¹,

Pietrzak²,

Chodorowska³

et al. 2019

pdia

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“…However, there are no definitive markers up to date that precisely identify LSCs although a variety of LSC markers have been proposed past years. For examples, nuclear factor p63 was previously identified as a stem cell marker for keratinocytes [ 13 ]; while later studies showed that p63 was not exclusive one and it expressed more wildly not only in LSC, but also in some transit amplifying cells, especially proliferative epithelial cells during cultures [ 14 ]. More efforts are necessary for researchers to focus on the identification of new molecular factors that maintain or determine the fate of these adult stem cells.…”

Section: Introductionmentioning

confidence: 99%

Unique Expression Pattern and Functional Role of Periostin in Human Limbal Stem Cells

Chi

Hua

et al. 2015

PLoS ONE

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Periostin is a non-structural matricellular protein. Little is known about periostin in human limbal stem cells (LSCs). This study was to explore the unique expression pattern and functional role of periostin in maintaining the properties of human LSCs. Fresh donor corneal tissues were used to make cryosections for evaluation of periostin expression on ex vivo tissues. Primary human limbal epithelial cells (HLECs) were generated from limbal explant culture. In vitro culture models for proliferation and epithelial regeneration were performed to explore functional role of periostin in LSCs. The mRNA expression was determined by reverse transcription and quantitative real-time PCR (RT-qPCR), and the protein production and localization were detected by immunofluorescent staining and Western blot analysis. Periostin protein was found to be exclusively immunolocalized in the basal layer of human limbal epithelium. Periostin localization was well matched with nuclear factor p63, but not with corneal epithelial differentiation marker Keratin 3. Periostin transcripts was also highly expressed in limbal than corneal epithelium. In primary HLECs, periostin expression at mRNA and protein levels was significantly higher in 50% and 70% confluent cultures at exponential growth stage than in 100% confluent cultures at slow growth or quiescent condition. This expression pattern was similar to other stem/progenitor cell markers (p63, integrin β1 and TCF4). Periostin expression at transcripts, protein and immunoreactivity levels increased significantly during epithelial regeneration in wound healing process, especially in 16-24 hours at wound edge, which was accompanied by similar upregulation and activation of p63, integrin β1 and TCF4. Our findings demonstrated that periostin is exclusively produced by limbal basal epithelium and co-localized with p63, where limbal stem cells reside. Periostin promotes HLEC proliferation and regeneration with accompanied activation of stem/progenitor cell markers p63, integrin β1 and TCF4, suggesting its novel role in maintaining the phenotype and functional properties of LSC.

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Differential expression of p63 isoforms in normal skin and hyperproliferative conditions

Cited by 4 publications

References 11 publications

Stem cell recovering effect of copper‐free GHK in skin

Stem cell recovering effect of copper‐free GHK in skin

Ectrodactyly-ectodermal dysplasia-clefting syndrome with unusual cutaneous vitiligoid and psoriasiform lesions due to a novel single point TP63 gene mutation

Unique Expression Pattern and Functional Role of Periostin in Human Limbal Stem Cells

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