Differential expression of preprosomatostatin- and somatostatin receptor-encoding mRNAs in association with the growth hormone-insulin-like growth factor system during embryonic development of rainbow trout (Oncorhynchus mykiss)

Malkuch, Heidi; Walock, Chad; Kittilson, Jeffery D.; Raine, Jason C.; Sheridan, Mark A.

doi:10.1016/j.ygcen.2008.08.005

Cited by 17 publications

(6 citation statements)

References 36 publications

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“…Elevated expression of three grouper SS genes was detected through developing embryos to hatch-out larvae (Ye et al, 2005). All three of forms of SS genes were also detected in embryos of rainbow trout (Malkuch et al, 2008). In Atlantic cod, SS1 was first detected in the pre-hatching stage and appeared to gradually increase with the development of cod embryos and larvae (Xu and Volkoff, 2009).…”

Section: Discussionmentioning

confidence: 93%

Molecular and expression characterization of two somatostatin genes in the Chinese sturgeon, Acipenser sinensis

Wei

Zhou

et al. 2009

Comparative Biochemistry and Physiology Part A: Molecular &

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Section: Discussionmentioning

confidence: 93%

Molecular and expression characterization of two somatostatin genes in the Chinese sturgeon, Acipenser sinensis

Wei

Zhou

et al. 2009

Comparative Biochemistry and Physiology Part A: Molecular &

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“…This is manifested as different levels of a particular subtype among tissues as well as differences in the relative abundance of subtypes within a particular tissue (Fukamachi et al 2005, Saera-Vila et al 2005, Very et al 2005, Ozaki et al 2006, Li et al 2007, Pierce et al 2007. Second, the pattern of expression of GHRs subtypes changes over the course of embryonic development and sexual maturation , Raine et al 2007, Malkuch et al 2008. Third, numerous perturbations, including fasting and seawater transfer, alter the differential expression profiles of GHR subtypes (Saera-Vila et al 2005, Norbeck et al 2007, Pierce et al 2007, Poppinga et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Differential ligand binding and agonist-induced regulation characteristics of the two rainbow trout GH receptors, Ghr1 and Ghr2, in transfected cells

Reindl

Kittilson

Sheridan

2009

Journal of Endocrinology

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Previously, we isolated and characterized two distinct GH receptor (GHR)-encoding mRNAs, ghr1 and ghr2, from rainbow trout. In this study, Chinese hamster ovary-K1 cells were individually transfected with plasmids that contained cDNAs encoding rainbow trout ghr1 or ghr2. High affinity binding of 125 I-salmonid GH (sGH) by the expressed receptors was saturable, displaceable, and ligand selective. Whole-cell binding analysis revealed a single class of binding site; for Ghr1While salmonid prolactin (sPrl) displaced 125 I-sGH from both Ghr1 and Ghr2, the affinity of either receptor subtype for sPrl was substantially less than for sGH; salmonid somatolactin, another member of the GH-PRL family, did not displace labeled sGH except at pharmacological concentrations.125 I-sGH was internalized by Ghr1-and Ghr2-expressing cells in a time-dependent manner; the maximum internalization reached was 71% for Ghr1 and 55% for Ghr2. Long-term exposure (24 h) of transfected cells to sGH up-regulated surface expression of both Ghr1 and Ghr2; however, sGH induced surface expression of Ghr1 to a greater extent than that of Ghr2. These results indicate that rainbow trout ghrs display both overlapping and distinct characteristics that may be important for ligand selection and differential action in target organs.

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“…It should be noted, however, that GH signaling in fish, such as rainbow trout, is particularly complex (more complex than in mammals) as a result of the existence of multiple GHRs that have differential ligand binding and agonist-induced regulation features (25,31). Moreover, the pattern of expression of the distinct GHRs of fish are affected by developmental and nutritional state and environmental salinity (15,19,21,23,24,28). Ultimately, therefore, the action of GH in fish may depend on the distribution and abundance of a specific GHR subtype as well as on the signal pathways to which they link under a given developmental/ physiological state.…”

Section: Perspectives and Significancementioning

confidence: 99%

Growth hormone-stimulated insulin-like growth factor-1 expression in rainbow trout (Oncorhynchus mykiss) hepatocytes is mediated by ERK, PI3K-AKT, and JAK-STAT

Reindl

Kittilson

Bergan

et al. 2011

American Journal of Physiology-Regulatory, Integrative and Comp

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Growth hormone (GH) initiates many of its growth-promoting actions by binding to GH receptors (GHR) and stimulating the synthesis and secretion of insulin-like growth factor-1 (IGF-1) from the liver and other sites. In this study, we used hepatocytes isolated from rainbow trout as a model system in which to determine the molecular signaling events of GH in fish. GH directly stimulated the phosphorylation of ERK, protein kinase B (Akt), a downstream target of phosphatidylinositol 3-kinase (PI3K), JAK2, and STAT5 in hepatocytes incubated in vitro. Activation of ERK, Akt, JAK2, and STAT5 was rapid, occurring within 5-10 min, and was concentration dependent. GH-induced ERK activation was completely blocked by the ERK pathway inhibitor, U0126, and the JAK2 inhibitor, 1,2,3,4,5,6-hexabromocyclohexane (Hex), and was partially blocked by the PI3K inhibitor LY294002. GH-stimulated Akt activation was completely blocked by LY294002 and Hex, but was not affected by U0126; whereas, STAT5 activation by GH was blocked only by Hex, and was not affected by either U0126 or LY294002. GH stimulated hepatic expression of IGF-1 mRNA as well as the secretion of IGF-1, effects that were partially or completely blocked by U0126, LY294002, and Hex. These results indicate that GHR linkage to the ERK, PI3K/Akt, or STAT pathways in trout liver cells requires activation of JAK2, and that GH-stimulated IGF-1 synthesis and secretion is mediated through the ERK, PI3K/Akt, and JAK-STAT pathways.

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Differential expression of preprosomatostatin- and somatostatin receptor-encoding mRNAs in association with the growth hormone-insulin-like growth factor system during embryonic development of rainbow trout (Oncorhynchus mykiss)

Cited by 17 publications

References 36 publications

Molecular and expression characterization of two somatostatin genes in the Chinese sturgeon, Acipenser sinensis

Molecular and expression characterization of two somatostatin genes in the Chinese sturgeon, Acipenser sinensis

Differential ligand binding and agonist-induced regulation characteristics of the two rainbow trout GH receptors, Ghr1 and Ghr2, in transfected cells

Growth hormone-stimulated insulin-like growth factor-1 expression in rainbow trout (Oncorhynchus mykiss) hepatocytes is mediated by ERK, PI3K-AKT, and JAK-STAT

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