Differential expression of prohibitin is correlated with dual action of Vitamin D as a proliferative and antiproliferative hormone in breast epithelial cells

Xiang, Peng; Mehta, Rajendra G.

doi:10.1016/j.jsbmb.2006.11.017

Cited by 7 publications

(4 citation statements)

References 12 publications

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“…Indeed, PHB1 and PHB2 protein levels were overexpressed in tumor cells isolated from patients with leukemia and lymphoma compared to normal naïve PBMCs (Figure 5 ). Previous reports suggest PHB1 mRNA levels are inversely proportional to cellular proliferation in a number of cell types [ 20 , 50 , 51 ], however the results presented herein indicate PHB1 and PHB2 are upregulated during the oxidative stress response in hematologic tumor cells.…”

Section: Discussioncontrasting

confidence: 86%

The prohibitin protein complex promotes mitochondrial stabilization and cell survival in hematologic malignancies

et al. 2017

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Prohibitins (PHB1 and PHB2) have been proposed to play important roles in cancer development and progression, however their oncogenic mechanism of action has not been fully elucidated. Previously, we showed that the PHB1 and PHB2 protein complex is required for mitochondrial homeostasis and survival of normal human lymphocytes. In this study, novel evidence is provided that indicates mitochondrial prohibitins are overexpressed in hematologic tumor cells and promote cell survival under conditions of oxidative stress. Immunofluorescent confocal microscopy revealed both proteins to be primarily confined to mitochondria in primary patient lymphoid and myeloid tumor cells and tumor cell lines, including Kit225 cells. Subsequently, siRNA-mediated knockdown of PHB1 and PHB2 in Kit225 cells significantly enhanced sensitivity to H2O2-induced cell death, suggesting a protective or anti-apoptotic function in hematologic malignancies. Indeed, PHB1 and PHB2 protein levels were significantly higher in tumor cells isolated from leukemia and lymphoma patients compared to PBMCs from healthy donors. These findings suggest that PHB1 and PHB2 are upregulated during tumorigenesis to maintain mitochondrial integrity and therefore may serve as novel biomarkers and molecular targets for therapeutic intervention in certain types of hematologic malignancies.

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Section: Discussioncontrasting

confidence: 86%

The prohibitin protein complex promotes mitochondrial stabilization and cell survival in hematologic malignancies

et al. 2017

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“…Since epidemiological studies have established an inverse correlation of reduced serum level of 25(OH)D 3 with increased cancer risk Gorham et al, 1990;Ainsleigh, 1993;Lipkin and Newmark, 1999;Cross et al, 2003;Friedrich et al, 2003;Grant and Garland, 2004;Lowe et al, 2005], more laboratory work is needed to investigate the functional role of 25(OH)D 3 . On the basis of the literature and our previous work [Peng and Mehta, 2007;Peng et al, 2009], we focused on evaluating the role of 25(OH)D 3 in stressmediated alterations. This is the first report on the anti-stress function of 25(OH)D 3 in breast epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

Protection against cellular stress by 25‐hydroxyvitamin D₃ in breast epithelial cells

Xiang

Vaishnav

Murillo

et al. 2010

J of Cellular Biochemistry

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25-Hydroxyvitamin D(3) (25(OH)D(3)) is a prohormone and a major vitamin D metabolite. The discovery of (25(OH)D(3)) 1 alpha-hydroxylase in many vitamin D target organs has yielded an increased interest in defining the role(s) of 25(OH)D(3) in these tissues. The etiology of cancer appears to be complex and multi-factorial. Cellular stress (e.g., DNA damage, hypoxia, oncogene activation) has been identified as one of the key factors responsible for initiating the carcinogenesis process. In this study, we investigated whether 25(OH)D(3) protects breast epithelial cells from cellular stress using an established breast epithelial cell line MCF12F. To better elucidate the role of 25(OH)D(3) in the stress response, we used multiple in vitro stress models including serum starvation, hypoxia, oxidative stress, and apoptosis induction. Under all these stress conditions, 25(OH)D(3) (250 nmol/L) treatment significantly protected cells against cell death. Low-serum stress induced p53 expression accompanied with downregulation of PCNA, the presence of 25(OH)D(3) consistently inhibited the alteration of p53 and PCNA, suggesting that these molecules were involved in the stress process and may be potential target genes of 25(OH)D(3). miRNA microarray analysis demonstrated that stress induced by serum starvation caused significant alteration in the expression of multiple miRNAs including miR182, but the presence of 25(OH)D(3) effectively reversed this alteration. These data suggest that there is a significant protective role for 25(OH)D(3) against cellular stress in the breast epithelial cells and these effects may be mediated by altered miRNA expression.

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“…Post treatment, the effects of the AHP-AuNP-KLA were more pronounced on Caco-2 cells (express PHB on cell surface) than the other two control cell lines as shown in Figure 4. 83 Although MCF-7 cells express PHB, 112 the cells were spared from the AHP-AuNP-KLA therapeutic effect as the cells express PHB in the cytoplasm. 83 These data further demonstrated the specificity of the ligand to PHB, and the potential AHP and other ligands (mentioned in Table 1) might have when used for PHB-targeted therapy.…”

Section: Potential Application Of Nanotechnology In Obesitymentioning

confidence: 99%

Vascular targeted nanotherapeutic approach for obesity treatment

Sibuyi¹,

Meyer²,

Onani³

et al. 2018

IJN

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Obesity is a global epidemic that poses a serious health concern due to it being a risk factor for life-threatening chronic diseases, such as type 2 diabetes, cancer, and cardiovascular diseases. Pharmacotherapy remains the mainstay for the management of obesity; however, its usefulness is limited due to poor drug efficacy, non-specificity and toxic side effects. Therefore, novel approaches that could provide insights into obesity and obesity-associated diseases as well as development of novel anti-obesity treatment modalities or improvement on the existing drugs are necessary. While the ideal treatment of obesity should involve early intervention in susceptible individuals, targeted nanotherapy potentially provides a fresh perspective that might be better than the current conventional therapies. Independent studies have shown improved drug efficacy by using prohibitin (PHB)-targeted therapy in obese rodents and non-human primates, thus providing a proof of concept that targeted nanotherapy can be a feasible treatment for obesity. This review presents a brief global survey of obesity, its impact on human health, its current treatment and their limitations, and the role of angiogenesis and PHB in the development of obesity. Finally, the role and potential use of nanotechnology coupled with targeted drug delivery in the treatment of obesity are discussed.

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Differential expression of prohibitin is correlated with dual action of Vitamin D as a proliferative and antiproliferative hormone in breast epithelial cells

Abstract: Our previous microarray analysis showed that N-methyl-N-nitrosourea transformed MCF12F breast epithelial cells exhibited upregulation of several genes, including prohibitin, which was reversed by

Cited by 7 publications

References 12 publications

The prohibitin protein complex promotes mitochondrial stabilization and cell survival in hematologic malignancies

The prohibitin protein complex promotes mitochondrial stabilization and cell survival in hematologic malignancies

Protection against cellular stress by 25‐hydroxyvitamin D₃ in breast epithelial cells

Vascular targeted nanotherapeutic approach for obesity treatment

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Differential expression of prohibitin is correlated with dual action of Vitamin D as a proliferative and antiproliferative hormone in breast epithelial cells

Abstract: Our previous microarray analysis showed that N-methyl-N-nitrosourea transformed MCF12F breast epithelial cells exhibited upregulation of several genes, including prohibitin, which was reversed by

Cited by 7 publications

References 12 publications

The prohibitin protein complex promotes mitochondrial stabilization and cell survival in hematologic malignancies

The prohibitin protein complex promotes mitochondrial stabilization and cell survival in hematologic malignancies

Protection against cellular stress by 25‐hydroxyvitamin D3 in breast epithelial cells

Vascular targeted nanotherapeutic approach for obesity treatment

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Product

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Protection against cellular stress by 25‐hydroxyvitamin D₃ in breast epithelial cells