Differential expression of proteins induced by lead in the Dwarf Sunflower Helianthus annuus

Walliwalagedara, Chamari; Atkinson, I.; Keulen, Harry van; Cutright, Teresa J.; Wei, Robert

doi:10.1016/j.phytochem.2010.05.018

Cited by 27 publications

(15 citation statements)

References 39 publications

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“…Another protein identified in these experiments is Mn-dependent superoxide dismutase (SOD) (spot 5), which catalyzes the reduction of superoxide anions to hydrogen peroxide. The increase of this enzyme might be to reduce oxidative stress, which results in production of reactive oxygen species, an observation that also was made using the Dwarf Sunflower [36], a similar observation was made when Chlamydomonas was exposed to Cd [23]. When cells were exposed to 400 µM arsenate a different SOD was identified namely a Fe-dependent SOD (spot extra 1).…”

Section: Proteomicssupporting

confidence: 54%

“…Heat shock protein 90B that was expressed as spot 15 is also a chaperonin protein and it has been reported to be expressed under metal stress [41,42]. Previously it was shown that a heat shock protein was up-regulated in Dwarf Sunflowers upon exposure to lead [36]. Ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) (spot 8) is a major enzyme in green plants that catalyzes carbon assimilation, hence controls photosynthetic rate and thus has a major effect on plant growth.…”

Section: Proteomicsmentioning

confidence: 99%

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Differential Proteome Analysis of <i>Chlamydomonas reinhardtii</i> Response to Arsenic Exposure

Walliwalagedara¹,

Keulen

Willard³

et al. 2012

AJPS

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The fresh water unicellular green alga Chlamydomonas reinhardtii was used to explore whether it could function as a model system to identify proteins that are differentially expressed in response to arsenate exposure. Cells were treated with different concentrations of arsenate ranging from 100 -400 M. When exposed to 200 M arsenate, the amount of live cells started to lessen on the second day and continued to diminish, indicating a toxic effect of arsenate. Proteomic analysis was used to investigate if these cells showed a specific response to arsenic-induced stress. Fifteen proteins were found that were over-expressed in the 200 M arsenate-treated samples and two proteins were found to be very strongly over-expressed in samples treated with 400 µM. These were selected for identification using liquid chromatography coupled with tandem mass spectrometry. Oxidative stress and protein damage were the major effects as shown by the up-regulation of Mn-superoxide dismutase, an oxygen-evolving enhancer protein, a chaperonin-like protein and a heat shock protein.

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Section: Proteomicssupporting

confidence: 54%

Section: Proteomicsmentioning

confidence: 99%

Differential Proteome Analysis of <i>Chlamydomonas reinhardtii</i> Response to Arsenic Exposure

Walliwalagedara¹,

Keulen

Willard³

et al. 2012

AJPS

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“…The efficiency of this remediation process depends on a number of factors from soil properties, metal bioavailability and speciation to the type of plant species. However, high concentration of absorbed metals usually ends up in the shoot biomass of the plant in harvestable form [12]. A number of recent studies reported various plant species that demonstrate phytoextraction strategy from both water and soil media [29][30][31][32].…”

Section: Phytoextractionmentioning

confidence: 99%

“…It is cheap, promotes biodiversity, reduces erosion, less destructive and decreased energy consumption leading to reduced carbon dioxide emission [11]. To date, about 400 plant species were suggested to be metal hyper-accumulators [12]. However, few studies reported the toxicity of several metals combined [13], and while hyperaccumulation of nickel (Ni), cadmium (Cd), manganese (Mn), zinc (Zn) and selenium (Se) have been well established, the same is yet be available or demonstrated beyond doubt in plant species for copper (Cu), chromium (Cr), lead (Pb), thallium (Th) and cobalt (Co) metals.…”

Section: Introductionmentioning

confidence: 99%

Phytoremediation: Halophytes as Promising Heavy Metal Hyperaccumulators

Usman¹,

Abu-Dieyeh²

2018

Heavy Metals

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The continued accumulation of trace and heavy metals in the environment presents a significant danger to biota health, including humans, which is undoubtedly undermining global environmental sustainability initiatives. Consequently, the need for efficient remediation technologies becomes imperative. Phytoremediation is one of the most viable options in this regard. Hundreds of plants in laboratory experiments demonstrate the potential to remediate varying concentrations of heavy metals; however, the remediation capacity of most of these plants proved unsatisfactory under field conditions. The identification and selection of plants with higher metal uptake capacity or hyperaccumulators are one of the limitations of this technology. Additionally, the mechanism of heavy metal uptake by plants remains to be sufficiently documented. The halophyte plants are famous for their adaptation to harsh environmental conditions, and hence could be the most suitable candidates for heavy metal hyperaccumulation. The state of Qatar in the Gulf region encompasses rich resources of halophytes that have the potential for future investment toward human and environmental health. This chapter, therefore, gives an overview of phytoremediation, with emphasis on halophytes as suitable heavy metal hyperaccumulators for improved remediation of heavy metal-contaminated areas.

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“…RuBisCObağlayıcı altbirim, RuBisCO büyük altbiriminin katlanması için gerekli bir proteindir (Boston et al 1996). RuBisCO aktivaz, RuBisCO'nun katalitik aktivitesini sağlamaktadır (Portis 2003 Patterson et al 2007;Zhen et al 2007;Führs et al 2008;Farinati et al 2009;Zhang et al 2009;Walliwalagedara et al 2010;Cai et al 2011 Metal stresine cevap olarak RuBisCO proteininin miktarındaki azalma birçok bitki türünde rapor edilmiştir (Hajduch et al 2001;Führs et al 2008;Kieffer et al 2009;Ahsan et al 2010;Zhao et al 2011). Bununla birlikte, RuBisCO alt birimlerindeki azalma Cd stresine maruz kalan hiperakümülatör Thlaspi caerulescens bitkilerinde de belirlenmiştir (Tuomainen et al 2006).…”

Section: Karbondioksit öZümlemesi Ve Fotosentez Ile İlişkili Proteinlerunclassified

Heavy Metal Toxicity in Plants: Proteomics Approach

Terzi¹,

Yıldız²

2013

AKU-J. Sci. Eng.

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ÖzetAğır metal kirliliği, insan sağlığını olumsuz etkileyen ve tarımsal verimde kayıplara neden olan önemli çevresel tehlikelerden biridir. Bitki hücresinin en önemli fonksiyonu, kendi savunması için ağır metal stresine karşı cevap vermektir. Ağır metal stresine cevap olarak fonksiyonel genler veya proteinlerin teşhisi stres cevaplarının moleküler mekanizmalarının anlaşılmasında önemli bir basamaktır. Protein ekspresyonu sadece transkripsiyonel seviyede değil, aynı zamanda translasyonel ve post-translasyonel seviyelerde de düzenlenmektedir. Bu nedenle, farklı stres koşulları altında bitki proteomundaki değişimleri araştırmak önemlidir. Genom tarafından eksprese olan proteinlerin geniş ölçekli analizi olarak tanımlanan proteomik, sadece proteomun tamamını tanımlamak için değil, aynı zamanda ağır metal stresi gibi farklı fizyolojik koşullar altındaki proteomların karşılaştırılması için güçlü bir moleküler araçtır. Bu derlemede, proteomik teknolojileri ve bitkilerde farklı metabolik olaylarda rolü olan ağır metal teşvikli proteinler tartışılmıştır. Heavy Metal Toxicity in Plants: Proteomics Approach Key wordsPlants; Heavy Metal; Toxicity; Proteomics; Mass Spectrometry AbstractHeavy metal contamination is one of the major environmental hazards, leading to losses in agricultural yields and harmfully affecting human health. The most crucial function of plant cell is to respond against heavy metal stress for self-defence. The identification of the functional genes or proteins that are involved in responses to heavy-metal stress is a fundamental step in understanding the molecular mechanisms of stress responses. Protein expression is regulated not only at the transcriptional level, but also at the translational and post-translational levels. Therefore, it is crucial to investigate the changes in plant proteome under different stress conditions. Proteomics, defined as the large-scale analysis of proteins expressed by the genome, is not only a powerful molecular tool for describing complete proteome, but also for comparative proteomes under different physiological conditions, such as heavy metal stress. In this review, proteomics technologies, and heavy metal induced proteins which play a role in different metabolic events in plants are discussed.

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Differential expression of proteins induced by lead in the Dwarf Sunflower Helianthus annuus

Cited by 27 publications

References 39 publications

Differential Proteome Analysis of <i>Chlamydomonas reinhardtii</i> Response to Arsenic Exposure

Differential Proteome Analysis of <i>Chlamydomonas reinhardtii</i> Response to Arsenic Exposure

Phytoremediation: Halophytes as Promising Heavy Metal Hyperaccumulators

Heavy Metal Toxicity in Plants: Proteomics Approach

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Differential expression of proteins induced by lead in the Dwarf Sunflower Helianthus annuus

Cited by 27 publications

References 39 publications

Differential Proteome Analysis of &lt;i&gt;Chlamydomonas reinhardtii&lt;/i&gt; Response to Arsenic Exposure

Differential Proteome Analysis of &lt;i&gt;Chlamydomonas reinhardtii&lt;/i&gt; Response to Arsenic Exposure

Phytoremediation: Halophytes as Promising Heavy Metal Hyperaccumulators

Heavy Metal Toxicity in Plants: Proteomics Approach

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Resources

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Differential Proteome Analysis of <i>Chlamydomonas reinhardtii</i> Response to Arsenic Exposure

Differential Proteome Analysis of <i>Chlamydomonas reinhardtii</i> Response to Arsenic Exposure