Differential expression of ten candidate genes regulating prostaglandin action in reproductive tissues of buffalo during estrous cycle and pregnancy

Verma, Ankita; Panigrahi, Manjit; Baba, Naseer Ahmad; Sulabh, Sourabh; Sadam, Abdul; Parida, Subhashree; Krishnaswamy, Narayanan; Sonwane, Arvind; Bhushan, Bharat

doi:10.1016/j.theriogenology.2017.09.008

Cited by 7 publications

(4 citation statements)

References 57 publications

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“…reported that women patient may develop late-onset PDP symptoms with atypical phenotype ( 45 ). Considering the importance of prostaglandin transporter in the regulation of prostaglandin action in the menstrual cycle of human female ( 22 , 46 , 47 ), we postulated that some hormones might protect female from the severe symptom of PHO in skin and bones, which hinted a potential hormone therapeutics for the temporary unaffected preadolescent male probands. Meanwhile, sex hormones would be responsible for the difference in the prostaglandin metabolism in women of reproductive age and in women in menopause.…”

Section: Discussionmentioning

confidence: 99%

Novel SLCO2A1 mutations cause gender-differentiated pachydermoperiostosis

Yuan

Chen

Liu

et al. 2018

Endocrine Connections

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Primary hypertrophic osteoarthropathy (PHO) is a rare familial disorder with reduced penetrance for females. The genetic mutations associated with PHO have been identified in HPGD and SLCO2A1, which involved in prostaglandin E2 metabolism. Here, we report 5 PHO patients from four non-consanguineous families. Two heterozygous mutations in solute carrier organic anion transporter family member 2A1 (SLCO2A1) were identified in two brothers by whole-exome sequencing. Three heterozygous mutations and one homozygous mutation were identified in other three PHO families by Sanger sequencing. However, there was no mutation in HPGD. These findings confirmed that homozygous or compound heterozygous mutations of SLCO2A1 were the pathogenic cause of PHO. A female individual shared the same mutations in SLCO2A1 with her PHO brother but did not have any typical PHO symptoms. The influence of sex hormones on the pathogenesis of PHO and its implication were discussed.

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Section: Discussionmentioning

confidence: 99%

Novel SLCO2A1 mutations cause gender-differentiated pachydermoperiostosis

Yuan

Chen

Liu

et al. 2018

Endocrine Connections

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“…The amplification was carried out in 20 µl volume reaction mixture containing 10 µl (2×) master mix (Fast SYBR Green qRT‐PCR Master Mix), 1 µl (10 µM) gene‐specific forward and reverse primers, 2 µl cDNA template and 6µl nuclease‐free water and by following thermal profile of Fast SYBR Green Master Mix: 95°C for 20 s, followed by 40 cycles of 95°C for 3 s and 57–60°C for 30 s. Non‐template control was included in each run. The GAPDH gene, a housekeeping gene, was used as an endogenous control for the analysis of data as it is the most widely used (Davies et al, ; Lee et al, ; Sadam et al, ; Verma et al, ). Hence, relative expression of (i) GAPDH , as endogenous control, and (ii) LGALS1 , LGALS3BP , LGALS9 , SLC2A1 , SLC5A11 , MUC1, CTSL and CST3 genes as target genes was carried out, in both pregnancy stages as well as in the nonpregnant stage (calibrator group).…”

Section: Methodsmentioning

confidence: 99%

“…Reduced P 4 concentration contributes to 50% embryonic loss in buffaloes (Jerome, & Srivastava, ). Recently, we have also conducted a comparative study on mRNA expression of genes related to prostaglandin (PG) synthesis, transport and effector pathways during different reproductive stages in buffalo (Verma et al, ). Based on the above facts, we studied the differential expression of P 4 ‐regulated genes such as LGALS1, LGALS3BP , LGALS9 , SLC2A1 , SLC5A11 , CTSL , MUC1 and CST3 during day 28‐56 of gestation in the buffalo.…”

Section: Introductionmentioning

confidence: 99%

Endometrial transcript profile of progesterone‐regulated genes during early pregnancy of Water Buffalo (Bubalus bubalis)

Baba

Panigrahi

Verma

et al. 2018

Reprod Domestic Animals

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Progesterone (P ) plays a key role in the establishment and maintenance of pregnancy in most mammals. Unravelling the expression of progesterone-regulated genes can expand the understanding of the embryonic mortality. Accordingly, we studied the relative mRNA expression of the P -regulated genes in the buffalo. Uteri were collected from the abattoir and categorized into nonpregnant late luteal phase, stage I (28-38th days of gestation) and stage II (48-56th days of gestation) of pregnancy (n = 6/group). After extraction of total RNA from the endometrial tissues, we carried out qRT-PCR for determining the relative mRNA expression of the P -regulated genes using nonpregnant late luteal phase as calibrator group. The expression of LGALS3BP (essential for maternal recognition of pregnancy) gene was found to be significantly upregulated (p < 0.05), while MUC1 (important for embryo attachment) gene was downregulated in stage I and II of pregnancy. We observed no significant change in the expression of LGALS1, LGALS9 and CTSL genes. The SLC5A11 and SLC2A1 genes (involved in the transport of glucose to endometrium) in early pregnancy were upregulated in the pregnancy stage I (p < 0.05) relative to nonpregnant late luteal phase. The CST3 gene was significantly upregulated in pregnancy stage II (p < 0.01). These results provide molecular insights into the specific pathways involved in foeto-maternal communication during early pregnancy in buffaloes.

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“…Expression of the COX-1 and COX-2 enzymes during gestation increases in uterine tissue, as has been demonstrated in sheep models [ 5 ]. Both of these isoforms are fundamental for producing PGs, such as PGF2α and PGE2, which are catalyzed by COX-2 to transform arachidonic acid into PGG2 and final products such as PGF2α, PGE2, and PGH2 by means of peroxides [ 8 , 74 ]. In mice, direct inhibition of COX-2 expression negatively regulates PGF2α synthesis during gestation, triggering the indirect suppression of the expression of c-Fos, which is considered a transcriptional activator of COX-2 [ 75 ] that is essential for inducing oxytocin receptors in the myometrium [ 76 ].…”

Section: Non-steroidal Anti-inflammatory Drugsmentioning

confidence: 99%

Analgesia during Parturition in Domestic Animals: Perspectives and Controversies on Its Use

Mota-Rojas

Velarde

Marcet-Rius

et al. 2022

Animals

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This article analyzes the physiological role of pain during parturition in domestic animals, discusses the controversies surrounding the use of opioids, non-steroidal anti-inflammatory drugs (NSAIDs), and local analgesics as treatments during labor, and presents the advantages and disadvantages for mother and offspring. Labor is a potentially stressful and painful event, due to the contractions that promote expulsion of the fetus. During labor, neurotransmitters such as the prostaglandins contribute to the sensitization of oxytocin receptors in the myometrium and the activation of nociceptive fibers, thus supporting the physiological role of pain. Endogenously, the body secretes opioid peptides that modulate harmful stimuli and, at the same time, can inhibit oxytocin’s action in the myometrium. Treating pain during the different stages of parturition is an option that can help prevent such consequences as tachycardia, changes in breathing patterns, and respiratory acidosis, all of which can harm the wellbeing of offspring. However, studies have found that some analgesics can promote myometrial contractility, increase expulsion time, affect fetal circulation, and alter mother–offspring recognition due to hypnotic effects. Other data, however, indicate that reducing the number of uterine contractions with analgesics increases their potency, thus improving maternal performance. Managing pain during labor requires understanding the tocolytic properties of analgesics and their advantages in preventing the consequences of pain.

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Differential expression of ten candidate genes regulating prostaglandin action in reproductive tissues of buffalo during estrous cycle and pregnancy

Cited by 7 publications

References 57 publications

Novel SLCO2A1 mutations cause gender-differentiated pachydermoperiostosis

Novel SLCO2A1 mutations cause gender-differentiated pachydermoperiostosis

Endometrial transcript profile of progesterone‐regulated genes during early pregnancy of Water Buffalo (Bubalus bubalis)

Analgesia during Parturition in Domestic Animals: Perspectives and Controversies on Its Use

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