Differential expression of the Brunol/CELF family genes during Xenopus laevis early development

Wu, Jingyang; Li, Chaocui; Song, Zhao; Mao, Bingyu

doi:10.1387/ijdb.082685jw

Cited by 25 publications

(34 citation statements)

References 27 publications

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“…Semi-quantitative reverse transcription PCR was carried out as previously described (Wu et al, 2010). The PCR primers and conditions were: XZFP36L1A: forward: 5'-TGCATGAGCAGCAGACGT G-3' and reverse: 5'-GGTCTTATGCGTCTCTCCATG-3', 30 cycles; XZFP36L1B: forward: 5'-TGCATGAGCAGCAGAGGA A-3' and reverse: 5'-GCCCTTCTGTGTCTCTAAACA-3', 30 cycles; H4 was used as a loading control: forward: 5'-CGGGA TAACATTCAGGGTA-3' and reverse: 5'-TCCATGGCG GTAACTGTC-3', 26 cycles.…”

Section: In Situ Hybridization Section and Rt-pcr Analysismentioning

confidence: 99%

“…Hela cell culture, transfection, and immunohistochemistry were carried out as described in previous research (Wu et al, 2010).…”

Section: Cell Transfection and Immunohistochemistrymentioning

confidence: 99%

“…The probes of X. laevis N-Tubulin (Papalopulu & Kintner, 1996), Sox2 (Ma et al, 2007), Slug (Aybar et al, 2003), Xath1 (Kim et al, 1997), En2 (Hemmati-Brivanlou et al, 1991), Pax3 (Sato et al, 2005), Dbx2 (Ma et al, 2011), and Nkx6.2 (Zhao et al, 2007) were used as previously described. Stained embryos were embedded in paraffin, sectioned at 20 μm, and photographed using a light microscope (Leica).Semi-quantitative reverse transcription PCR was carried out as previously described (Wu et al, 2010). The PCR primers and conditions were: XZFP36L1A: forward: 5'-TGCATGAGCAGCAGACGT G-3' and reverse: 5'-GGTCTTATGCGTCTCTCCATG-3', 30 cycles; XZFP36L1B: forward: 5'-TGCATGAGCAGCAGAGGA A-3' and reverse: 5'-GCCCTTCTGTGTCTCTAAACA-3', 30 cycles; H4 was used as a loading control: forward: 5'-CGGGA TAACATTCAGGGTA-3' and reverse: 5'-TCCATGGCG GTAACTGTC-3', 26 cycles.…”

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confidence: 99%

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Involvement of XZFP36L1, an RNA-binding protein, in <I>Xenopus</I> neural development

Xia¹,

Song²,

Mao³

2013

Zoological Research

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Xenopus ZFP36L1 (zinc finger protein 36, C3H type-like 1) belongs to the ZFP36 family of RNA-binding proteins, which contains two characteristic tandem CCCH-type zinc-finger domains. The ZFP36 proteins can bind AU-rich elements in 3' untranslated regions of target mRNAs and promote their turnover. However, the expression and role of ZFP36 genes during neural development in Xenopus embryos remains largely unknown. The present study showed that Xenopus ZFP36L1 was expressed at the dorsal part of the forebrain, forebrain-midbrain boundary, and midbrain-hindbrain boundary from late neurula stages to tadpole stages of embryonic development. Overexpression of XZFP36L1 in Xenopus embryos inhibited neural induction and differentiation, leading to severe neural tube defects. The function of XZP36L1 requires both its zinc finger and C terminal domains, which also affect its subcellular localization. These results suggest that XZFP36L1 is likely involved in neural development in Xenopus and might play an important role in post-transcriptional regulation.Keywords: ZFP36L1; RNA-binding protein; Neural development; Xenopus; Post-transcriptional regulation mRNA stability is an important mechanism for gene expression regulation, which is critical for embryogenesis and normal physiological processes. The zinc finger protein 36 (ZFP36) family proteins are key players in post-transcriptional regulation and contain two or more highly conserved tandem CCCH zinc fingers (TZF) of tandem Cx8Cx5Cx3H repeats (x represents a variable amino acid). These factors are able to bind to AU-rich elements (AREs) in the 3' untranslated regions (UTR) of targeted mRNAs through their TZFs Lai & Blackshear, 2001;Lai et al, 1999;Lai et al, 2000), which leads to the removal of the poly(A) tail off the targeted mRNAs and promotes their degradation (Carrick et al, 2004;Lai & Blackshear, 2001). The ZFP36 family proteins are evolutionarily conserved, including four rodent members (ZFP36, ZFP36L1, ZFP36L2, and ZFP36L3) and three mammal members (with ZFP36L3 missing, Blackshear et al, 2005), each of which has two conserved ZF domains. Four members have been reported in Xenopus and zebrafish, among which ZFP36, ZFP36L1, and ZFP36L2 are conserved while the fourth member (Xenopus ZFP36L2.1, ZFP36L2.2 and zebrafish ZFCTH1) contains four ZF domains instead of two. The four ZFP36 genes in Xenopus have been cloned and are widely expressed in adult tissues, except for XZFP36L2.1 (XC3H-4), which is detected only in the ovary (De et al, 1999). It has been suggested that XZFP36L2b (XC3H-3b) plays an important role in Xenopus kidney development (Kaneko et al, 2003), but little is known about the roles of other ZFP36 members in embryonic development. 1Also known as cMG1, TIS11b, ERF1, BRF1, C3H-2, and Berg36 (Barnard et al, 1993;De et al, 1999;Lai et al, 1990;Varnum et al, 1991), ZFP36L1 has been reported to regulate vascular endothelial growth factor (VEGF) mRNA stability in adrenocorticotropic hormone-stimulated primary adrenocortical cells acting as a negative...

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Section: In Situ Hybridization Section and Rt-pcr Analysismentioning

confidence: 99%

“…Hela cell culture, transfection, and immunohistochemistry were carried out as described in previous research (Wu et al, 2010).…”

Section: Cell Transfection and Immunohistochemistrymentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Involvement of XZFP36L1, an RNA-binding protein, in <I>Xenopus</I> neural development

Xia¹,

Song²,

Mao³

2013

Zoological Research

Self Cite

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“…The RRMs confer RNA binding activity, and it is postulated that the divergent linker domain is an important site for functional regulation. Six members of the CELF family have been identified in humans and mice: CELF1 (CUGBP1) and CELF2 (CUGBP2) proteins are expressed ubiquitously and play vital role in embryogenesis [35], [36], [37], [38], [39], whereas CELF proteins 3-6 are restricted to adult tissues and found almost exclusively in the nervous system [40], [41]. CELF proteins often serve multiple functions in both the cytoplasm and the nucleus [42], [43].…”

Section: Evolutionary Conservation Of Celf Proteinsmentioning

confidence: 99%

CELF1, a Multifunctional Regulator of Posttranscriptional Networks

Beisang¹,

Bohjanen²,

Louis³

2012

Binding Protein

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“…The ELAV/Hu related family Brunol/CELF genes are also differentially expressed during neurogenesis and are essential for proper neural development (Wu et al, 2010). During neurogenesis, several genes such as the Sox family of transcription factors, neurexin (nrxn), and CRMP gene also present differential expression pattern suggesting differential regulation (Cunningham et al, 2008;Souopgui et al, 2007;Zeng et al, 2006).…”

Section: Alternative Forms Of Elrd Transcriptsmentioning

confidence: 99%

Regulation and expression of elrD1 and elrD2 transcripts during early Xenopus laevis development

Nassar¹

2011

Int. J. Dev. Biol.

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The Xenopus laevis elrD (elav-like ribonucleoprotein D) gene is a member of the elav/ Hu family which encodes RNA-binding proteins. Most of the elav/Hu genes are expressed in the nervous system, where they are implicated in the development and maintenance of neurons. The regulation of elrD gene expression involves two promoters, pD1 and pD2. In this study, we analyzed the neural specificity directed by both promoters. They were fused to the gene encoding green fluorescent protein, and their ability to drive neural expression in injected Xenopus embryos was examined. We show that both promoters direct neural expression and that whole promoter sequences are needed to induce neural specific expression. Finally, we analyzed the spatial and temporal localization of the two elrD transcripts, elrD1 and elrD2. We found that the two transcripts present the same tissue-specific pattern of expression, with distinct developmental regulation. Our results show a complex regulation of the elrD gene and suggest that different transcripts resulting from alternative splicing of the elrD gene probably define different neurons. KEY WORDS: regulation, promoter, alternative splicing, elav, XenopusThe elav/Hu genes constitute a multigenic family implicated in the post-transcriptional control of gene expression in neuronal cells. In Drosophila, three members of the elav/Hu have been isolated: elav (Yao et al., 1993), rbp9 (Kim and Baker, 1993) and fne (Samson and Chalvet, 2003). Members of this family have also been identified in human (Szabo et al., 1991), in the mouse Darnell, 1997), in Xenopus (Good, 1995), in chickens (Wakamatsu and Weston, 1997), in zebrafish (Park et al., 2000) and in Caenorhabditis elegans (Fujita et al., 1999). Expression of elav/Hu genes is generally restricted to all or parts of the nervous system, except for elrB/HuB whose transcripts are also expressed in the ovary and testis and elrA/HuA whose transcripts are ubiquitously expressed (Good, 1995;Okano and Darnell, 1997;Wakamatsu and Weston, 1997).All elav/Hu genes encodes RNA-binding proteins (RBPs) containing three RNA recognition motifs (RRMs). RRM-containing proteins are known to be involved in many post-transcriptional and translational events .In Drosophila, genetic analysis of elav suggests a role in the differentiation and maintenance of neurons (Yao et al., 1993). Consistently, overexpression of a wild type form of HuB or HuC in the mouse embryonic neural tube induces ectopic expression of neuronal markers, whereas a dominant negative form suppresses Int. J. Dev. Biol. 55: 127-132 (2011) Abbreviations used in this paper: elrD1, elav-like ribonucleoprotein D 1; elrD2, elav-like ribonucleoprotein D 2; pD1, elrD1 promoter; pD2, elrD2 promoter; GFP, green fluorescent protein.

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Differential expression of the Brunol/CELF family genes during Xenopus laevis early development

Cited by 25 publications

References 27 publications

Involvement of XZFP36L1, an RNA-binding protein, in <I>Xenopus</I> neural development

Involvement of XZFP36L1, an RNA-binding protein, in <I>Xenopus</I> neural development

CELF1, a Multifunctional Regulator of Posttranscriptional Networks

Regulation and expression of elrD1 and elrD2 transcripts during early Xenopus laevis development

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