Differential expression of the fibroin gene in developmental stages of silkworm, Antheraea mylitta (Saturniidae)

Datta, Abhik; Ghosh, Ananta K.; Kundu, Subhas C.

doi:10.1016/s1096-4959(01)00377-3

Cited by 67 publications

(35 citation statements)

References 26 publications

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“…For tissue engineering applications, the surface must have adhesive property to attach the cells properly. Antheraea mylitta silk protein fibroin molecules have specific sequences (AAN28165) similar to RGD sequences which provides better attachment sites for cells as reported earlier (Dutta et al, 2001b). These sequences are similar to that present in case of Antheraea pernyi, a wild non-mulberry temperate oak tasar silkworm belonging to Saturniidae (Minoura et al, 1995).…”

Section: Cell Adhesion Viability and Proliferation Of Fibroblasts Onsupporting

confidence: 55%

Non‐bioengineered silk gland fibroin protein: Characterization and evaluation of matrices for potential tissue engineering applications

Mandal

Kundu

2008

Biotech & Bioengineering

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The possibility of using wild non-mulberry silk protein as a biopolymer remains unexplored compared to domesticated mulberry silk protein. One of the main reasons for this was for not having any suitable method of extraction of silk protein fibroin from cocoons and silk glands. In this study non-bioengineered non-mulberry silk gland fibroin protein from tropical tasar silkworm Antheraea mylitta, is regenerated and characterized using 1% (w/v) sodium dodecyl sulfate (SDS). The new technique is important and unique because it uses a mild surfactant for fibroin dissolution and is advantageous over other previous reported techniques using chaotropic salts. Fabricated fibroin films are smooth as confirmed by atomic force microscopy. Circular dichroism spectrometry along with Fourier transformed infrared spectroscopy and X-ray diffraction reveal random coil/alpha-helix conformations in regenerated fibroin which transform to beta-sheets, resulting in crystalline structure and protein insolubility through ethanol treatment. Differential scanning calorimetry shows an increase in glass transition (Tg) temperature and enhanced degradation temperature on alcohol treatment. Enhanced cell attachment and viability of AH927 feline fibroblasts were observed on fibroin matrices. Higher mechanical strength along with controllable water stability of regenerated gland fibroin films make non-mulberry Indian tropical tasar silk gland fibroin protein a promising biomaterial for tissue engineering applications.

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Section: Cell Adhesion Viability and Proliferation Of Fibroblasts Onsupporting

confidence: 55%

Non‐bioengineered silk gland fibroin protein: Characterization and evaluation of matrices for potential tissue engineering applications

Mandal

Kundu

2008

Biotech & Bioengineering

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“…2b), but after 12 h, cells were more spreaded on the surface of A. mylitta films than on TCP, Matrigel and B. mori fibroin films. The spreading of cells on the surface of B. mori fibroin films was found to be less than on the other matrices, probably due to the presence of topological and biochemical differences [50,51] as discussed in Section 3.1.…”

Section: Confluency and Spreading Of Mda-mb-231 Cells On 2d Coated Plmentioning

confidence: 95%

Engineered silk fibroin protein 3D matrices for in vitro tumor model

et al. 2011

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“…This region was highly conserved in the Rff and Ayf genes (92.0%). The coding region was 42 bp long, and encoded a highly conserved amino acid sequence with 14 amino acid residues, 13 of which were identical with Rff and B. mori FHC (Zhou et al, 2000) and 12 with Rff and the three Antheraea fibroins (Tamura et al, 1987;Sezutsu & Yukuhiro, 2000;Datta et al, 2001;Fig. 3a).…”

Section: Conserved Nature Of First Exonic Sequencesmentioning

confidence: 99%

Leucine-rich fibroin gene of the Japanese wild silkmoth, Rhodinia fugax (Lepidoptera: Saturniidae)

Sezutsu¹,

Tamura²,

Yukuhiro³

2008

Eur. J. Entomol.

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Abstract. We cloned and characterized a partial fibroin gene of Rhodinia fugax (Saturniidae). The gene encodes a fibroin consisting mainly of orderly arranged repeats, each of which is divided into a polyalanine and a nonpolyalanine block, similar to the fibroins of Antheraea pernyi and A. yamamai. Three repeat types differ in the sequence of the nonpolyalanine block. In contrast to the Antheraea fibroins, the fibroin of R. fugax is rich in glutamate and leucine residues (about 3% and 5%, respectively) and contains less alanine.

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Differential expression of the fibroin gene in developmental stages of silkworm, Antheraea mylitta (Saturniidae)

Cited by 67 publications

References 26 publications

Non‐bioengineered silk gland fibroin protein: Characterization and evaluation of matrices for potential tissue engineering applications

Non‐bioengineered silk gland fibroin protein: Characterization and evaluation of matrices for potential tissue engineering applications

Engineered silk fibroin protein 3D matrices for in vitro tumor model

Leucine-rich fibroin gene of the Japanese wild silkmoth, Rhodinia fugax (Lepidoptera: Saturniidae)

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