Differential expression of TLP, ERF1, and R2R3MYB in annual Medicago species under salinity conditions

Abstract

ABSTRACT. The present study was conducted to evaluate the responses of three annual Medicago species (M. truncatula, M. laciniata, and M. polymorpha) to salinity. We analyzed publicly available microarray data in NCBI pertaining to salinity-response genes in M. truncatula. Our data search identified Tubby C2 (TLP) and ethylene responsive transcription factor 1 (ERF1) as genes that potentially respond to salinity. We evaluated morpho-physiological traits and the expression of the genes in three Medicago species that had been maintained under control and saline conditions. The analysis of morpho-physiological traits showed that M. polymorpha and M. laciniata were more tolerant to salinity than M. truncatula, as they had lower reductions in biomass and dry root weight and lower increases in anthocyanin concentration. The saline conditions caused a significant increase (P < 0.01) in the expression of TLP in all Medicago species, but caused a significant decrease 10152-10164 (2015) in the expression of ERF1. Considerable variation in anthocyanin concentrations was found among the three Medicago species. To investigate the cause of this variation, we examined the expression of R2R3MYB, a gene involved in the biosynthesis of anthocyanins. Our analysis showed that saline conditions induced high over-expression of R2R3MYB in all three Medicago spp. The high efficiency of the primer pairs used in qRT-PCR enabled us to compare the expression levels of each gene in the three species. We concluded that the more salt tolerant species showed higher expression of TLP and R2R3MYB under both control and salinity conditions.

A tubby-like protein CsTLP8 acts in the ABA signaling pathway and negatively regulates osmotic stresses tolerance during seed germination

“…In medicago, barley, maize, cassava, apple and chickpea, TLPs were reported to play a role in response to abiotic stress treatments or hormone treatment [11,[16][17][18][19][20]. Ectopically expressed MdTLP7 increased growth activity of E. coli under NaCl, KCl, chilling, and heat treatment [21], and overexpression of full length or truncated MdTLP7 (containing only the tubby domain) enhanced the stress tolerance of Arabidopsis to abiotic stresses [22], suggesting that MdTLP7 functions in response to abiotic stress and the tubby domain of MdTLP7 plays a key role in this response.…”

Arabidopsis Tubby domain‐containing F‐box proteins positively regulate immunity by modulating PI4Kβ protein levels

“…Rice TLPs have been implicated in biotic stress responses as their transcription was upregulated upon pathogen infection ( Cai et al, 2008 ; Kou et al, 2009 ). In a number of studies based on large-scale transcriptional analysis in response to stresses, TLPs have been implicated in both biotic and abiotic stress responses such as responses to high and cold temperatures, salinity, exogeneous ABA, salt, and drought, in Malus domesticus , Medicago truncatula , Triticum aestivum , and Zea mays ( Gharaghani et al, 2015 ; Hong et al, 2016 ; Xu et al, 2016 ; Yulong et al, 2016 ). Although these studies point to the importance of TLPs in various plant stress responses, the mechanism by which the TLPs are functioning remains elusive.…”

Potential application of light emitting diodes to improve molecular, physiological, and growth responses in peppermint plant

“…Temperature gradient PCR was used to find the optimum annealing temperature (Table 1). Reactions lacking a cDNA template were also performed at the optimized conditions as a negative control 27,28 . PCR reaction mixture contained 4 μL of 8X diluted cDNA, 5 μL of SYBR Green Master Mix ( NEB , UK), 0.5 μL of forward primers, and 0.5 μL of reverse primers.…”

“…Reactions lacking a cDNA template were also performed at the optimized conditions as a negative control. 27,28 PCR reaction mixture contained 4 μL of 8X diluted cDNA, 5 μL of SYBR Green Master Mix (NEB, UK), 0.5 μL of forward primers, and 0.5 μL of reverse primers. Each biological replicate had three technical replications in RT-qPCR.…”