Differential Expression of Two Copies of the
            <i>irmA</i>
            Gene in the Enteroaggregative E. coli Strain 042

Bernabeu, Manuel; Aznar, Salvador; Prieto, Alejandro; Hüttener, Mário; Juárez, Antonio

doi:10.1128/spectrum.00454-22

Cited by 2 publications

(1 citation statement)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The dimer structure is formed by a unique domain swap mechanism where the C-terminal unstructured region and β-strand from each protomer complete the FNIII-like fold of the other ( 14 ). It is produced by uropathogenic Escherichia coli (UPEC), a causative agent of urinary tract infections (UTIs), and some strains of diarrheagenic E. coli ( 14 , 15 ). UTIs affect over half of all women during their lifetime with the associated antimicrobial treatment accounting for 15% of outpatient prescriptions ( 16 ).…”

Section: Introductionmentioning

confidence: 99%

Efficient Autotransporter-Mediated Extracellular Secretion of a Heterologous Recombinant Protein by Escherichia coli

et al. 2023

View full text Add to dashboard Cite

Recombinant protein production is an essential component of the biotechnology sector. Here, we show that the autotransporter platform is a viable method for the recombinant production, secretion, and purification of a “difficult” to produce protein on an industrially relevant scale. Use of the autotransporter platform could reduce the number of downstream processing operations required, thus accelerating the development time and reducing costs for recombinant protein production.

show abstract

Section: Introductionmentioning

confidence: 99%

Efficient Autotransporter-Mediated Extracellular Secretion of a Heterologous Recombinant Protein by Escherichia coli

et al. 2023

View full text Add to dashboard Cite

show abstract

Evolution of two metabolic genes involved in nucleotide and amino acid metabolism in Pseudomonas aeruginosa

Wu,

Shi,

Liang

2024

PLoS ONE

View full text Add to dashboard Cite

Pseudomonas aeruginosa is an opportunistic human pathogen causing various severe infections. Understanding genetic mechanisms of its metabolic versatility aids in developing novel antibacterial drugs and therapeutic strategies to address multidrug-resistant P. aeruginosa infections. The metabolism of nucleotides and amino acids contributes to the cycle of two key biological macromolecules in the genetic central dogma. Guanine deaminase (GuaD) catalyzes the deamination of guanine to produce xanthine to maintain the homeostasis of the nucleotide pool, and transporters specific to BCAAs (termed as BraT) import BCAAs to keep its intracellular availability level. However, little is known about the evolution of GuaD and BraT in P. aeruginosa population. Here, two copies turned out to be widespread in P. aeruginosa population for each of GuaD and BraT. The phylogenic analysis demonstrated that GuaD1 and BraB were inherited from the ancestor of Pseudomonas, while GuaD2 and BraZ were additionally acquired via evolutionary events in the ancestors of P. aeruginosa. The functional divergence of two copies was supported by different distribution patterns of dN/dS ratios, divergent expression levels, differentially co-expressed genes, and their functional enrichment modules with few intersections. Besides, some co-expressed genes with known functions are involved in infecting hosts, forming biofilm and resisting antibiotic treatment. Taken together, functional divergence following copy number increase and differentiation of co-expression networks might confer greater metabolic potential to P. aeruginosa, especially in response to host immune responses and antibiotic treatments in clinical settings.

show abstract

Differential Expression of Two Copies of the irmA Gene in the Enteroaggregative E. coli Strain 042

Abstract: Gene duplications occur in prokaryotic genomes at a detectable frequency. In many instances, the biological function of the duplicates is unknown, and hence, the significance of the presence of multiple copies of these genes remains unclear.

Cited by 2 publications

References 50 publications

Efficient Autotransporter-Mediated Extracellular Secretion of a Heterologous Recombinant Protein by Escherichia coli

Efficient Autotransporter-Mediated Extracellular Secretion of a Heterologous Recombinant Protein by Escherichia coli

Evolution of two metabolic genes involved in nucleotide and amino acid metabolism in Pseudomonas aeruginosa

Contact Info

Product

Resources

About