Differential expression of two pro-opiomelanocortin mRNAs during temperature stress in common carp (Cyprinus carpio L.)

Arends, R.J.; Gaag, R van der; Martens, Gerard J.M.; Bonga, S.E. Wendelaar; Flik, G.

doi:10.1677/joe.0.1590085

Cited by 53 publications

(37 citation statements)

References 26 publications

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“…We analysed the transcript abundance of pomca1 and pomcb1, paralogues that originate from the salmonid genome duplication (Arends et al, 1998;Leder and Silverstein, 2006;Macqueen and Johnston, 2014;Salbert et al, 1992). No changes in pomca1 and pomcb1 mRNA transcripts were observed when fish were subjected to either UCS or the novel stressor.…”

Section: Pituitary Glandmentioning

confidence: 99%

Stress in Atlantic salmon: response to unpredictable chronic stress

Madaro

Olsen

Kristiansen

et al. 2015

Journal of Experimental Biology

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Combinations of stressors occur regularly throughout an animal's life, especially in agriculture and aquaculture settings. If an animal fails to acclimate to these stressors, stress becomes chronic, and a condition of allostatic overload arises with negative results for animal welfare. In the current study, we describe effects of exposing Atlantic salmon parr to an unpredictable chronic stressor (UCS) paradigm for 3 weeks. The paradigm involves exposure of fish to seven unpredictable stressors three times a day. At the end of the trial, experimental and control fish were challenged with yet another novel stressor and sampled before and 1 h after that challenge. Plasma cortisol decreased steadily over time in stressed fish, indicative of exhaustion of the endocrine stress axis. This was confirmed by a lower cortisol response to the novel stressor at the end of the stress period in chronically stressed fish compared with the control group. In the preoptic area (POA) and pituitary gland, chronic stress resulted in decreased gene expression of 11βhsd2, gr1 and gr2 in the POA and increased expression of those genes in the pituitary gland. POA crf expression and pituitary expression of pomcs and mr increased, whereas interrenal gene expression was unaffected. Exposure to the novel stressor had no effect on POA and interrenal gene expression. In the pituitary, crfr1, pomcs, 11βhsd2, grs and mr were downregulated. In summary, our results provide a novel overview of the dynamic changes that occur at every level of the hypothalamicpituitary gland-interrenal gland (HPI) axis as a result of chronic stress in Atlantic salmon.

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Section: Pituitary Glandmentioning

confidence: 99%

Stress in Atlantic salmon: response to unpredictable chronic stress

Madaro

Olsen

Kristiansen

et al. 2015

Journal of Experimental Biology

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“…Plasma concentrations of cortisol were determined in triplicate by RIA according to Arends et al (1998) using a commercial antiserum (Bioclinical Services Ltd, Cardiff, UK). The cortisol antibody cross-reactivity with 11-deoxycortisol, cortisone acetate, cortisone, and 17a-OH-progesterone was 5 .…”

Section: Plasma Cortisol Levelsmentioning

confidence: 99%

Pituitary hormone mRNA expression in European sea bass Dicentrarchus labrax in seawater and following acclimation to fresh water

Varsamos¹,

Xuereb²,

Commes³

et al. 2006

Journal of Endocrinology

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The mRNA expression of pituitary prolactin (prl), growth hormone (gh), somatolactin (sl), proopiomelanocortin (pomc), and gonadotropins (gthI and gthII) was quantified by real-time PCR, in sea bass, Dicentrarchus labrax, adapted for 1 month to seawater (SW) or freshwater (FW). In addition, IGF-I (igfI) mRNA expression in liver and branchial Na C /K C -ATPase activity were determined. L17 ribosomal protein (rpL17) and elongation factor 1a (ef1a) were validated as reference genes in real-time PCR in the experimental context. The real-time PCR assays were validated for the different hormone genes considered. Expression of pituitary pomc, gthI, gthII, gh, and liver igfI was not significantly different between FW and SW fish. Pituitary prl was 4 . 5-fold higher in FW than in SW, whereas pituitary sl was 1 . 8-fold higher in SW-compared with FW-adapted fish. Gill Na C /K C -ATPase specific activity was 2 . 3-fold higher in FW sea bass compared with SW fish. Plasma cortisol levels were 6 . 5-fold lower in SW-than in FW-adapted specimens. The results are discussed in relation to the osmoregulatory strategy of this euryhaline SW species, which displays features that do not fit present models based on salmonids and FW euryhaline teleosts.

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“…Plasma cortisol was measured by radioimmunoassay (RIA) as described by Arends et al (1998). Plasma osmolality was measured on a freezing-point depression osmometer (Gonotec, Berlin, Germany).…”

Section: Plasma Parametersmentioning

confidence: 99%

“…Our previous experiments show that carp acclimated to increasing water temperature show increased basal plasma cortisol levels (Arends et al, 1998). In the present study, we evaluate the hydromineral and endocrine consequences of acclimation to water at 15, 22 and 29°C.…”

mentioning

confidence: 91%

Regulation of branchial Na+/K+-ATPase in common carpCyprinus carpioL. acclimated to different temperatures

Metz

Burg

Bonga

et al. 2003

Journal of Experimental Biology

113

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Isogenic carp Cyprinus carpio L. were acclimated to water temperatures of 15, 22 and 29°C for at least 8 weeks. The acclimations consistently resulted in slightly, but significantly, different plasma osmolality, sodium, potassium and chloride concentrations between the groups studied. Plasma total and ionic calcium levels were unaffected, indicating successful adaptation. The apparent changes in set point for plasma ion levels are explained by altered sodium pump activity and hormonal control of branchial permeability to water and ions. It appears that in 15°C-acclimated fish, a lower apparent Na + /K + -ATPase activity is compensated by strongly enhanced Na + /K + -ATPase expression (determined biochemically and immunohistochemically). In 29°C-acclimated fish, the higher ambient temperature activates the enzyme. Arrhenius plots for branchial Na + /K + -ATPase preparations of the three groups of fish suggest the occurrence of different enzyme isoforms or protein (in)stability as explanations for differences in apparent enzyme activities, rather than temperature-dependent changes in membrane fluidity. As for hormonal control over permeability, prolactin mRNA expression (and anticipated production and release) is lower in fish kept at 29°C, suggesting that control over branchial permeability to water and ions needs to be downregulated at higher temperatures. In so doing, enhanced sodium pump activity is balanced by a controlled passive ion loss to fine-tune plasma sodium levels. Basal plasma cortisol levels did not correlate positively with Na + /K + -ATPase expression, but doubling plasma cortisol levels in control fish by administering exogenous cortisol (for 7 days, using implanted minipumps and thus stress-free) enhanced Na + /K + -ATPase expression. This effect must be the result of a glucocorticoid action of the steroid: in fish, mineralocorticoid receptors have higher affinity for cortisol than glucocorticoid receptors. At a lower ambient temperature, branchial Na + /K + -ATPase expression is upregulated to counteract the temperature-inhibited activity of the sodium pump, perhaps via a mineralocorticoid receptor.

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Differential expression of two pro-opiomelanocortin mRNAs during temperature stress in common carp (Cyprinus carpio L.)

Cited by 53 publications

References 26 publications

Stress in Atlantic salmon: response to unpredictable chronic stress

Stress in Atlantic salmon: response to unpredictable chronic stress

Pituitary hormone mRNA expression in European sea bass Dicentrarchus labrax in seawater and following acclimation to fresh water

Regulation of branchial Na+/K+-ATPase in common carpCyprinus carpioL. acclimated to different temperatures

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