Differential expression of Xenopus ribosomal protein gene XlrpS1c

Scholnick, Joshua; Sinor, Cleve; Oakes, Jennifer A.; Outten, Wayne; Saha, Margaret S.

doi:10.1016/s0167-4781(97)00101-2

Cited by 6 publications

(5 citation statements)

References 38 publications

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“…The extensive expression analysis of rpl5 throughout the embryonic development of Xenopus laevis showed a detailed expression in the neural tissue, such as the developing eye and brain, as well as the NCCs. These findings are consistent with previously published expression patterns of rpl5 in Xenopus laevis ( Scholnick et al, 1997 ; Wischnewski et al, 2000 ; Session et al, 2016 ). Furthermore, rpl5 expression is located in tissues which are mainly affected in patients suffering from ribosomopathies ( Narla and Ebert, 2010 ; Farley-Barnes et al, 2019 ).…”

Section: Discussionsupporting

confidence: 93%

“…Ribosomal Protein L5 is Specifically Expressed in the Developing Xenopus laevis Earlier, rpl5 expression was shown in whole embryos at stages 27 and 32 during Xenopus development (Scholnick et al, 1997;Wischnewski et al, 2000). Session et al provided RNAsequencing data showing rpl5 expression throughout the entire embryonic development (Session et al, 2016).…”

Section: Genomic Analysis Of Rpl5mentioning

confidence: 99%

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The Ribosomal Protein L5 Functions During Xenopus Anterior Development Through Apoptotic Pathways

Schreiner

Kernl

Dietmann

et al. 2022

Front. Cell Dev. Biol.

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Ribosomal biogenesis is a fundamental process necessary for cell growth and division. Ribosomal protein L5 (Rpl5) is part of the large ribosomal subunit. Mutations in this protein have been associated with the congenital disease Diamond Blackfan anemia (DBA), a so called ribosomopathy. Despite of the ubiquitous need of ribosomes, clinical manifestations of DBA include tissue-specific symptoms, e.g., craniofacial malformations, eye abnormalities, skin pigmentation failure, cardiac defects or liver cirrhosis. Here, we made use of the vertebrate model organism Xenopus laevis and showed a specific expression of rpl5 in the developing anterior tissue correlating with tissues affected in ribosomopathies. Upon Rpl5 knockdown using an antisense-based morpholino oligonucleotide approach, we showed different phenotypes affecting anterior tissue, i.e., defective cranial cartilage, malformed eyes, and microcephaly. Hence, the observed phenotypes in Xenopus laevis resemble the clinical manifestations of DBA. Analyses of the underlying molecular basis revealed that the expression of several marker genes of neural crest, eye, and brain are decreased during induction and differentiation of the respective tissue. Furthermore, Rpl5 knockdown led to decreased cell proliferation and increased cell apoptosis during early embryogenesis. Investigating the molecular mechanisms underlying Rpl5 function revealed a more than additive effect between either loss of function of Rpl5 and loss of function of c-Myc or loss of function of Rpl5 and gain of function of Tp53, suggesting a common signaling pathway of these proteins. The co-injection of the apoptosis blocking molecule Bcl2 resulted in a partial rescue of the eye phenotype, supporting the hypothesis that apoptosis is one main reason for the phenotypes occurring upon Rpl5 knockdown. With this study, we are able to shed more light on the still poorly understood molecular background of ribosomopathies.

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Section: Discussionsupporting

confidence: 93%

Section: Genomic Analysis Of Rpl5mentioning

confidence: 99%

The Ribosomal Protein L5 Functions During Xenopus Anterior Development Through Apoptotic Pathways

Schreiner

Kernl

Dietmann

et al. 2022

Front. Cell Dev. Biol.

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“…The size of the rplE-(H) high molecular mass band observed in our Northern blot analysis (Fig. 5h) (Scholnick et al, 1997) …”

Section: Discussionmentioning

confidence: 75%

cDNA–RNA subtractive hybridization reveals increased expression of mycocerosic acid synthase in intracellular Mycobacterium bovis BCG

Monahan

Waddell

et al. 2001

Microbiology

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Identifying genes that are differentially expressed by Mycobacterium bovis BCG after phagocytosis by macrophages will facilitate the understanding of the molecular mechanisms of host cell-intracellular pathogen interactions. To identify such genes a cDNA-total RNA subtractive hybridization strategy has been used that circumvents the problems both of limited availability of bacterial RNA from models of infection and the high rRNA backgrounds in total bacterial RNA. The subtraction products were used to screen a high-density gridded Mycobacterium tuberculosis genomic library. Sequence data were obtained from 19 differential clones, five of which contained overlapping sequences for the gene encoding mycocerosic acid synthase (mas). Mas is an enzyme involved in the synthesis of multi-methylated long-chain fatty acids that are part of phthiocerol dimycocerosate, a major component of the complex mycobacterial cell wall. Northern blotting and primer extension data confirmed up-regulation of mas in intracellular mycobacteria and also revealed a putative extended N10 promoter structure and a long untranslated upstream region 5' of the mas transcripts, containing predicted double-stranded structures. Furthermore, clones containing overlapping sequences for furB, groEL-2, rplE and fadD28 were identified and the up-regulation of these genes was confirmed by Northern blot analysis. The cDNA-RNA subtractive hybridization enrichment and high density gridded library screening, combined with selective extraction of bacterial mRNA represents a valuable approach to the identification of genes expressed during intra-macrophage residence for bacteria such as M. bovis BCG and the pathogenic mycobacterium, M. tuberculosis.

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“…However, there are now several reports of differential ribosomal gene expression in both animal tissues (Saha et al, 1993;Agrawal and Bowman, 1987) and Xenopus (Scholnick et al, 1997). Therefore, although use of salmon L18 ribosomal oligonucleotides provides a valid means of determining cDNA synthesis in each sample, it appears to be constantly yet differentially expressed throughout development and as such was not used for normalization purposes.…”

mentioning

confidence: 99%

Atlantic SalmonHNF-3/forkhead: cDNA Sequence, Evolution, Expression, and Functional Analysis

Stenson¹,

McNair²,

Byrnes³

et al. 2000

DNA and Cell Biology

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We report the isolation and characterization of a cDNA encoding an HNF-3 family member (as HNF-3) from Atlantic salmon (Salmo salar L). The important functional domains of HNF-3 proteins that have been characterized previously are revealed by segments of high identity along the alignment of the asHNF-3 with winged helix/forkhead amino acid sequences isolated from other species. A comparison of asHNF-3 cDNA and genomic DNA indicated that there were no introns present in the asHNF-3 gene. Expression of asHNF-3 protein in adult salmon tissues was not exclusive to liver but was also present in the pancreas and intestine. An RT-PCR analysis performed on salmon development showed that asHNF3 expression is detectable before gastrulation at the mid blastula transition stage. Functional analysis of the asHNF-3 protein using a characterized HNF-3 consensus binding site demonstrated that the protein can recognize and bind to specific HNF-3 consensus sequences. We also report the identification of a novel HNF3 binding site in the promoter of the Atlantic salmon transferrin gene.

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Differential expression of Xenopus ribosomal protein gene XlrpS1c

Cited by 6 publications

References 38 publications

The Ribosomal Protein L5 Functions During Xenopus Anterior Development Through Apoptotic Pathways

The Ribosomal Protein L5 Functions During Xenopus Anterior Development Through Apoptotic Pathways

cDNA–RNA subtractive hybridization reveals increased expression of mycocerosic acid synthase in intracellular Mycobacterium bovis BCG

Atlantic SalmonHNF-3/forkhead: cDNA Sequence, Evolution, Expression, and Functional Analysis

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