Differential Expression of Xylanases and Endoglucanases in the Hybrid Derived from Intergeneric Protoplast Fusion between a Cellulomonas sp. and Bacillus subtilis

Abstract

A stable hybrid obtained by protoplast fusion between a Cellulomonas sp. and Bacillus subtilis exhibits an altered pattern of enzyme induction with different cellulosic substrates. Unlike in the Cellulomonas sp., xylanase was induced in the hybrid organism specifically by xylan, and endoglucanase was induced by carboxymethyl cellulose. The amount and specific activity of xylanase produced by the hybrid were more than those produced by the Cellulomonas sp. P-Glucosidase which is cell bound or intracellular in the Cellulomonas sp. was secreted by the hybrid organism, and relative amounts of extracellular B-glucosidase were high.

Molecular biology of xylan degradation

“…Induction of xylobiose could not be analyzed as the B. subtilis enzyme is more active than that of B. pumilus. In E. coli both enzymes were produced constitutively• In a different approach to the analysis of gene expression, Gokhale and Deobagkar [101] studied hybrids derived from intergeneric protoplast fusion between a Cellulomonas sp. and B. subtilis.…”

Biocatalyst development for lactic acid production at acidic pH using inter-generic protoplast fusion

“…Protoplast fusion is the useful technique in the improvement of microorganisms. We have been successful in transferring multiple phenotypes using protoplast fusion approach leading to changed properties of enzymes such as cellulase 24,25 and amylase. 26 Here we describe its application to improve acid tolerance in one of the strains of Lactobacillus with commercial potential.…”

Effect of Bacillus subtilis BsuM restriction-modification on plasmid transfer by polyethylene glycol-induced protoplast fusion

“…Polyethylene glycol (PEG)-induced cell fusion is one of the means to transfer DNA between bacterial cells, and numerous instances have been reported for intraspecific, interspecific, and intergeneric protoplast fusion (Akamatsu & Sekiguchi, 1983;Chen et al, 1986Chen et al, , 1987Cocconcelli et al, 1986;Baigorí et al, 1988;van der Lelie et al, 1988;van der Vossen et al, 1988;Gokhale & Deobagkar, 1989). The genetic materials used in these studies include either plasmids or chromosomal DNA.…”

“…The genetic materials used in these studies include either plasmids or chromosomal DNA. Thus, the successful transfer of plasmids has been reported for interspecific gene transfer between Bacillus subtilis and Bacillus species (Akamatsu & Sekiguchi, ), and for intergeneric transfer between B. subtilis and Streptococcus lactis (van der Vossen et al ., ) and between Streptococcus and Lactobacillus cells (Gokhale & Deobagkar, ).…”