Differential Expression Profiles of microRNAs in NIH3T3 Cells in Response to UVB Irradiation

Guo, Ling; Huang, Zhong-Xi; Chen, Xiaowei; Qin-kai, Deng; Yan, Wei; Zhou, Meijuan; Ou, Chengshan; Ding, Zhenhua

doi:10.1111/j.1751-1097.2008.00482.x

Cited by 63 publications

(61 citation statements)

References 35 publications

(53 reference statements)

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“…Similarly, miR-18b overexpression promotes proliferation and loss of cell adhesion, and elevated levels of this miR correlates with reduced relapse-free survival in hepatocellular carcinoma (47). Finally, miR-365 expression is increased in breast cancer, as well as in response to UVB radiation (48,49). To our knowledge, there are no published clinical reports on miR and their role in predicting prostate cancer response to external beam radiotherapy or brachytherapy.…”

Section: Discussionmentioning

confidence: 85%

miRNA-95 Mediates Radioresistance in Tumors by Targeting the Sphingolipid Phosphatase SGPP1

et al. 2013

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Radiation resistance poses a major clinical challenge in cancer treatment, but little is known about how microRNA (miR) may regulate this phenomenon. In this study, we used next-generation sequencing to perform an unbiased comparison of miR expression in PC3 prostate cancer cells rendered resistant to fractionated radiation treatment. One miR candidate found to be upregulated by ionizing radiation was miR-95, the enforced expression of which promoted radiation resistance in a variety of cancer cells. miR-95 overexpression recapitulated an aggressive phenotype including increased cellular proliferation, deregulated G 2 -M checkpoint following ionizing radiation, and increased invasive potential. Using combined in silico prediction and microarray expression analyses, we identified and validated the sphingolipid phosphatase SGPP1, an antagonist of sphingosine-1-phosphate signaling, as a target of miR-95 that promotes radiation resistance. Consistent with this finding, cell treatment with FTY720, a clinically approved small molecule inhibitor of S1P signaling, sensitized miR-95 overexpressing cells to radiation treatment. In vivo assays extended the significance of these results, showing that miR-95 overexpression increased tumor growth and resistance to radiation treatment in tumor xenografts. Furthermore, reduced tumor necrosis and increased cellular proliferation were seen after radiation treatment of miR-95 overexpressing tumors compared with control tumors. Finally, miR-95 expression was increased in human prostate and breast cancer specimens compared with normal tissue. Together, our work reveals miR-95 expression as a critical determinant of radiation resistance in cancer cells. Cancer Res; 73(23); 6972-86. Ó2013 AACR.

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Section: Discussionmentioning

confidence: 85%

miRNA-95 Mediates Radioresistance in Tumors by Targeting the Sphingolipid Phosphatase SGPP1

et al. 2013

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“…miRNAs are evolutionarily conserved small noncoding RNAs that regulate gene expression and play important roles in diverse biological functions, including cell differentiation, proliferation, apoptosis, tumorigenesis, and the immune response (12)(13)(14)(15)(16)(17). Previous studies have shown that miRNA expression profiles change in response to UVB irradiation, and these changes were most pronounced within the first few hours following UVB exposure (23,24). Thus, we wanted to determine whether miRNA is involved in UVB-induced melanocyte dendricity at an early stage.…”

Section: Uvb Irradiation Promotes Melanocyte Dendricitymentioning

confidence: 99%

“…The miRNA expression profiles of UV-irradiated cells have been investigated using miRNA microarrays, and in one such study, differential miRNA expression profiles were found in UVBirradiated NIH 3T3 cells compared with nonirradiated cells (23). The miRNA expression changes were most pronounced within the first hours following UVB irradiation, suggesting that miRNAmediated gene regulation operates earlier than most transcriptional responses (24).…”

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confidence: 99%

MicroRNA 340 Is Involved in UVB-Induced Dendrite Formation through the Regulation of RhoA Expression in Melanocytes

Jin

Zhu

et al. 2014

Molecular and Cellular Biology

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The influence of UV irradiation on pigmentation is well established, but the molecular and cellular mechanisms controlling dendrite formation remain incompletely understood. MicroRNAs (miRNAs) are a class of small RNAs that participate in various cellular processes by suppressing the expression of target mRNAs. In this study, we investigated the expression of miRNAs in response to UVB irradiation using a microarray screen and then identified potential mRNA targets for differentially expressed miRNAs among the genes governing dendrite formation. We subsequently determined the ability of miRNA 340 (miR-340) to suppress the expression of RhoA, which is a predicted miR-340 target gene that regulates dendrite formation. The overexpression of miR-340 promoted dendrite formation and melanosome transport, and the downregulation of miR-340 inhibited UVBinduced dendrite formation and melanosome transport. Moreover, a luciferase reporter assay demonstrated direct targeting of RhoA by miR-340 in the immortalized human melanocyte cell line Pig1. In conclusion, this study has established an miRNA associated with UVB irradiation. The significant downregulation of RhoA protein and mRNA expression after UVB irradiation and the modulation of miR-340 expression suggest a key role for miR-340 in regulating UVB-induced dendrite formation and melanosome transport. Melanocytes originate from neural crest-derived melanoblasts, which migrate and differentiate in the basal layer of the epidermis (1). A hallmark of melanocytes is their ability to form dendrites, which are specialized cell structures that transport melanosomes to their tips for transfer to the surrounding keratinocytes in response to growth factors and UV irradiation. Following skin penetration by UV rays and subsequent DNA damage, thymidine dinucleotide fragments induce melanogenesis and cause the melanocyte to produce melanosomes (2).These melanosomes are then transferred to neighboring keratinocytes through the intricate network of melanocyte dendrites, contributing to skin darkening and thereby providing protection from UV radiation (3, 4). Melanocyte dendrites can vary markedly in length and number in response to different growth factors and, in a manner analogous to the way in which neural cells seek out target neurons, these dendrites form growth cone-like structures that attach to keratinocytes. Melanocyte-keratinocyte adhesion is a prerequisite for the transfer of melanosomes to keratinocytes; therefore, the formation of melanocyte dendrites, particularly of the appropriate length and number, is essential for efficient melanosome transfer. Due to the importance of dendricity for melanocyte activity, cutaneous pigmentation, and photoprotection, it is critical to determine the precise mechanisms involved in the regulation of melanocyte dendrite formation.It is well known that melanocytes are sensitive to UV irradiation, with substantial evidence suggesting that it plays a pivotal role in regulating melanocyte dendricity. Studies have shown that UV irradiation induce...

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“…Enlightening findings on the microRNA expression profile have been obtained in mouse fibroblast cell line NIH3T3 irradiated with UV-B. UV-B irradiation resulted in robust changes in the microRNA expression profile including the upregulation of let-7a, miR-21, and miR-24 and the downregulation of miR-465 (Guo et al, 2009). Subsequent work on microRNA expression in primary human keratinocytes exposed to UV-A or UV-B radiation revealed that UV-A induces miR-21, miR-203, and miR-205, whereas UV-B moderately induces miR-203, reduces miR-205 and had no effect on miR-21 (Dziunycz et al, 2010).…”

Section: Micrornasmentioning

confidence: 99%

UV-Induced Immune Suppression that Promotes Skin Cancer Development and Progression

Kato¹,

Wang²

2011

Skin Cancers - Risk Factors, Prevention and Therapy

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Differential Expression Profiles of microRNAs in NIH3T3 Cells in Response to UVB Irradiation

Cited by 63 publications

References 35 publications

miRNA-95 Mediates Radioresistance in Tumors by Targeting the Sphingolipid Phosphatase SGPP1

miRNA-95 Mediates Radioresistance in Tumors by Targeting the Sphingolipid Phosphatase SGPP1

MicroRNA 340 Is Involved in UVB-Induced Dendrite Formation through the Regulation of RhoA Expression in Melanocytes

UV-Induced Immune Suppression that Promotes Skin Cancer Development and Progression

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