Differential extraction of eleostearic acid‐rich lipid‐protein complexes in tung seeds

Abstract

Lipid-protein complexes were identified in the 104,000 × g supernatant fraction of developing tung seeds. Incubation of this fraction with linoleoyl-CoA promoted an increase of chloroform-extractable lipids in a time-dependent manner. High-performance liquid chromatography analysis indicated that the extracted lipids were similar to mature tung oil triglycerides. Differential extraction using chloroform or chloroform/methanol indicated that linoleoyl-CoA promoted extraction of pre-existing lipids rather than de novo synthesis. An increase in extractable lipids was also observed after incubation with proteinase K. Isolation of lipid-protein complexes by sucrose density centrifugation and analysis of proteins by gel electrophoresis revealed several proteins specifically associated with this lipid fraction. FIG. 1. High-performance liquid chromatography analysis of eleostearoyl lipids extracted after incubation of 104,000 × g supernatant with linoleoyl-CoA. (a) Tung oil standard. (b) Lipids extracted from the 104,000 × g supernatant after 20 min incubation. The three major peaks containing eleostearic acid are labeled 1, 2 and 3. (c) Changes in absorbance of each of the three major peaks observed in (b) throughout the course of the assay.

Effect of Dietary Tung Oil on the Growth and Lipid Metabolism of Laying Hens.

“…Tung oil, which has been used in the paint and var nish industries for many years, contains 70% to 80% eleostearic acid (9, 11, 13-octadecatrienoic acid; EA) of total fatty acids. It could be expected to be a resource of the functional conjugated fatty acids in animal feed be cause of its relatively low price and its higher EA con tent than other EA-rich oils, such as karela oil (17,18).…”