Differential Fecal Microbiome Dysbiosis after Equivalent Traumatic Brain Injury in Aged Versus Young Adult Mice

Davis, Booker T; Islam, Mecca B.A.R.; Das, Promi; Gilbert, Jack A.; Ho, Karen J.; Schwulst, Steven J.

doi:10.33696/neurol.2.044

Cited by 6 publications

(6 citation statements)

References 57 publications

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“…Moreover, reduced evenness and abundance of gut microbiota were reflected in Shannon, Simpson, and Fisher indices in both male and female mice. These alpha diversity-related changes in gut microbiota following CCI correspond with the earlier research findings ( Nicholson et al, 2019 ; Davis et al, 2021 , 2022 ; Du et al, 2021 ; Frankot et al, 2023 ). The bidirectional crosstalk of the brain and gut microbiota has also been evident in the management of brain injuries such as stroke, spinal cord injuries, and TBI ( Singh et al, 2016 ; Akhoundzadeh et al, 2018 ; Sun et al, 2018 ; Ma et al, 2019 ; Devi et al, 2021 ; Frankot et al, 2023 ).…”

Section: Discussionsupporting

confidence: 90%

“…We also noted the increased abundance of P. copri in the CCI_M → F group. In this way, sex-specific characterization of gut microbiota phenotypes may be useful for predicting post-TBI neurological recovery and crafting better therapeutic approaches ( Luczynski et al, 2016 ; Sundman et al, 2017 ; Davis et al, 2021 ; Du et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

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Fecal microbiota transplantation unveils sex-specific differences in a controlled cortical impact injury mouse model

Pasam,

Dandekar

2024

Front. Microbiol.

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IntroductionContusion type of traumatic brain injury (TBI) is a major cause of locomotor disability and mortality worldwide. While post-TBI deleterious consequences are influenced by gender and gut dysbiosis, the sex-specific importance of commensal gut microbiota is underexplored after TBI. In this study, we investigated the impact of controlled cortical impact (CCI) injury on gut microbiota signature in a sex-specific manner in mice.MethodsWe depleted the gut microflora of male and female C57BL/6 mice using antibiotic treatment. Thereafter, male mice were colonized by the gut microbiota of female mice and vice versa, employing the fecal microbiota transplantation (FMT) method. CCI surgery was executed using a stereotaxic impactor (Impact One™). For the 16S rRNA gene amplicon study, fecal boli of mice were collected at 3 days post-CCI (dpi).Results and discussionCCI-operated male and female mice exhibited a significant alteration in the genera of Akkermansia, Alistipes, Bacteroides, Clostridium, Lactobacillus, Prevotella, and Ruminococcus. At the species level, less abundance of Lactobacillus helveticus and Lactobacillus hamsteri was observed in female mice, implicating the importance of sex-specific bacteriotherapy in CCI-induced neurological deficits. FMT from female donor mice to male mice displayed an increase in genera of Alistipes, Lactobacillus, and Ruminococcus and species of Bacteroides acidifaciens and Ruminococcus gnavus. Female FMT-recipient mice from male donors showed an upsurge in the genus Lactobacillus and species of Lactobacillus helveticus, Lactobacillus hamsteri, and Prevotella copri. These results suggest that the post-CCI neurological complications may be influenced by the differential gut microbiota perturbation in male and female mice.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Fecal microbiota transplantation unveils sex-specific differences in a controlled cortical impact injury mouse model

Pasam,

Dandekar

2024

Front. Microbiol.

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“…Age, in the setting of TBI, has also been shown to affect dysbiosis as well. One study showed that older mice subjected to TBI had greater changes in disease-associated microbial species compared to younger mice counterparts [ 36 ]. Another group performed repetitive TBI on adolescent and adult male and female rats, showing that while there were no differences between age groups in terms of diversity or taxonomy at 17 or 30 days postinjury, pre-injury depletion of the microbiome resulted in a postinjury microbiome with a pathogenic proinflammatory profile, with decreases in commensal bacteria such as Bacteroides and Bifidobacterium , which was worse in adolescents [ 37 ].…”

Section: Traumamentioning

confidence: 99%

The Intestinal Microbiome after Traumatic Injury

et al. 2023

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The intestinal microbiome plays a critical role in host immune function and homeostasis. Patients suffering from—as well as models representing—multiple traumatic injuries, isolated organ system trauma, and various severities of traumatic injury have been studied as an area of interest in the dysregulation of immune function and systemic inflammation which occur after trauma. These studies also demonstrate changes in gut microbiome diversity and even microbial composition, with a transition to a pathobiome state. In addition, sex has been identified as a biological variable influencing alterations in the microbiome after trauma. Therapeutics such as fecal transplantation have been utilized to ameliorate not only these microbiome changes but may also play a role in recovery postinjury. This review summarizes the alterations in the gut microbiome that occur postinjury, either in isolated injury or multiple injuries, along with proposed mechanisms for these changes and future directions for the field.

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“…Notably, older mice subjected to TBI displayed different gut flora environments than younger mice. 154 Indeed, the microbiome may undergo a spectrum of changes over time following TBI, making analysis of gut flora a possible venue for injury prognostication. 155 However, TBI-induced changes in the gut microbiome may be alleviated through faecal transplant which, again, has been demonstrated to improve cognitive performance in mice.…”

Section: Traumatic Brain Injurymentioning

confidence: 99%

Gut microbiome and neurosurgery: Implications for treatment

Willman

Reddy

et al. 2022

Clinical and Translational Dis

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Introduction: The aim of this review is to summarize the current understanding of the gut-brain axis (GBA), its impact on neurosurgery, and its implications for future treatment. Background: An abundance of research has established the existence of a collection of pathways between the gut microbiome and the central nervous system (CNS), commonly known as the GBA. Complicating this relationship, the gut microbiome bacterial diversity appears to change with age, antibiotic exposure and a number of external and internal factors. Methods: In this paper, we present the current understanding of the key protective and deleterious roles the gut microbiome plays in the pathogenesis of several common neurosurgical concerns. Results: Specifically, we examine how spinal cord injury, traumatic brain injury and stroke may cause gut microbial dysbiosis. Furthermore, this link appears to be bidirectional as gut dysbiosis contributes to secondary CNS injury in each of these ailment settings. This toxic cycle may be broken, and the future secondary damage rescued by timely, therapeutic, gut microbiome modification. In addition, a robust gut microbiome appears to improve outcomes in brain tumour treatment. There are several primary routes by which microbiome dysbiosis may be ameliorated, including faecal microbiota transplant, oral probiotics, bacteriophages, genetic modification of gut microbiota and vagus nerve stimulation. Conclusion: The GBA represents an important component of patient care in the field of neurosurgery. Future research may illuminate ideal methods of therapeutic microbiome modulation in distinct pathogenic settings.

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Differential Fecal Microbiome Dysbiosis after Equivalent Traumatic Brain Injury in Aged Versus Young Adult Mice

Abstract: TBI symptoms in young patients is well documented in the literature demonstrating patterns of impairment in communication, behavior, higher-order cognition, and learning efficiency [10][11][12].

Cited by 6 publications

References 57 publications

Fecal microbiota transplantation unveils sex-specific differences in a controlled cortical impact injury mouse model

Fecal microbiota transplantation unveils sex-specific differences in a controlled cortical impact injury mouse model

The Intestinal Microbiome after Traumatic Injury

Gut microbiome and neurosurgery: Implications for treatment

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