Differential gene expression analysis of benzo(a)pyrene toxicity in the clam, Ruditapes philippinarum

Liu, Tong; Pan, Luqing; Qian, Jin; Cai, Yuefeng

doi:10.1016/j.ecoenv.2015.02.007

Cited by 16 publications

(9 citation statements)

References 59 publications

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“…Known for its pro-carcinogenic properties, benzo[a]pyrene (BaP) (C20H12) is thought to be one of the most toxic PAHs and is classified by the International Agency for Research on Cancer as a group 1 human carcinogen (IARC, 2011;Liu et al, 2015;Châtel et al, 2017). Formed through the incomplete pyrolysis of combustible organic material, the main sources are anthropogenically derived from fossil fuel combustion, waste incineration, and oil spills.…”

Section: Introductionmentioning

confidence: 99%

“…Formed through the incomplete pyrolysis of combustible organic material, the main sources are anthropogenically derived from fossil fuel combustion, waste incineration, and oil spills. PAHs emitted, as soot or gas, to the atmosphere, enter the marine environment through rain and surface run-off (Antunes et al, 2013;Liu et al, 2015;Châtel et al, 2017). BaP is ubiquitously distributed in coastal and marine environment (Antunes et al, 2013;Liu et al, 2015;Châtel et al, 2017) and has been used as a model to investigate the effects and metabolic pathways of PAHs in marine organisms (Liu et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Ecotoxicological Effects of Chemical Contaminants Adsorbed to Microplastics in the Clam Scrobicularia plana

et al. 2018

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Although microplastics (MPs) are distributed globally in the marine environment, a great deal of unknowns relating to their ecotoxicological effects on the marine biota remains. Due to their lipophilic nature, microplastics have the potential to adsorb persistent organic pollutants present in contaminated regions, which may increase their detrimental impact once assimilated by organisms. This study investigates the ecotoxicological effects of exposure to low-density polyethylene (LDPE) microplastics (11-13 µm), with and without adsorbed contaminants (benzo[a]pyrene-BaP and perfluorooctane sulfonic acid-PFOS), in the peppery furrow shell clam, Scrobicularia plana. Environmentally relevant concentrations of contaminants (BaP−16.87 ± 0.22 µg g −1 and PFOS−70.22 ± 12.41 µg g −1) were adsorbed to microplastics to evaluate the potential role of plastic particles as a source of chemical contamination once ingested. S. plana were exposed to microplastics, at a concentration of 1 mg L −1 , in a water-sediment exposure setup for 14 days. Clams were sampled at the beginning of the experiment (day 0) and after 3, 7, and 14 days. BaP accumulation, in whole clam tissues, was analyzed. A multi-biomarker assessment was conducted in the gills, digestive gland, and haemolymph of clams to clarify the effects of exposure. This included the quantification of antioxidant (superoxide dismutase, catalase, glutathione peroxidase) and biotransformation (glutathione-Stransferases) enzyme activities, oxidative damage (lipid peroxidation levels), genotoxicity (single and double strand DNA breaks), and neurotoxicity (acetylcholinesterase activity). Results suggest a potential mechanical injury of gills caused by ingestion of microplastics that may also affect the analyzed biomarkers. The digestive gland seems less affected by mechanical damage caused by virgin microplastic exposure, with the MPs-adsorbed BaP and PFOS exerting a negative influence over the assessed biomarkers in this tissue.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ecotoxicological Effects of Chemical Contaminants Adsorbed to Microplastics in the Clam Scrobicularia plana

et al. 2018

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“…Benzo[a]pyrene (BaP) is formed by incomplete combustion of organic materials (Collins et al, 1991) and has become the most frequently studied representative of carcinogenic PAHs and a model molecule for the investigation of PAHs as inducers of aryl hydrocarbon receptor (AhR)-based metabolization pathways. BaP is ubiquitously distributed in coastal and offshore environments (Antunes et al, 2013;Liu et al, 2015;Châtel et al, 2017), and PAHs including BaP are known to adsorb at high rates to different types of MPs in seawater, particularly to PE (Rochman et al, 2013a;Ziccardi et al, 2016;Schönlau et al, 2019). Furthermore, BaP adsorbed to MPs has been documented to be transferred via trophic transfer to biota (Batel et al, 2016(Batel et al, , 2018Bour et al, 2018;O'Donovan et al, 2018;Pittura et al, 2018).…”

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confidence: 99%

Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)

Cormier

Batel

Cachot

et al. 2019

Front. Environ. Sci.

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As wide-spread pollutants in the marine environment, microplastics (MPs) have raised public concern about potential toxic effects in aquatic organisms, and, among others, MPs were suspected to act as a vector for organic pollutants to biota. The purpose of the present study was to investigate effects by three model pollutants, oxybenzone (BP3), benzo[a]pyrene (BaP), and perfluorooctane sulfonate (PFOS) adsorbed to polyethylene MPs on the basis of a standard assay, the acute fish embryo toxicity test (FET; OECD TG 236) with zebrafish (Danio rerio) supplemented by additional endpoints such as induction of ethoxyresorufin-O-deethylase (EROD) activity, modification of cyp1a gene transcription and changes in larval swimming behavior. FET assays were performed in three laboratories using slightly different husbandry and exposure conditions, which, however, were all fully compatible with the limits defined by OECD TG 236. This allowed for testing of potential changes in the FET assay due to protocol variations. The standard endpoints of the FET (acute embryotoxicity) did not reveal any acute toxicity for both virgin MPs and MPs spiked with BP3, BaP, and PFOS. With respect to sublethal endpoints, EROD activity was increased after exposure to MPs spiked with BP3 (3 h pulse) and MPs spiked with BaP (96 h continuous exposure). Cyp1a transcription was increased upon exposure to MPs spiked with BP3 or BaP. For the selected combination of MPs particles and contaminants, the basic FET proved not sensitive enough to reveal effects of (virgin and spiked) MPs. However, given that the FET can easily be supplemented by a broad variety of more subtle and sensitive endpoints, an enhanced FET protocol may provide a relevant approach with developmental stages of a vertebrate animal model, which is not protected by current EU animal welfare legislation (Directive EU 2010/63).

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“…cant increment (16.1 times) was observed with extra naphthalene supplementation; whereas a statistically reliable increase (by 3.6 times) in sodC transcription, we found only in bacteria incubated in naphthalene-enriched media.4 | D ISCUSS I ONVarious manifestations of oxidative stress in the presence of polyaromatic hydrocarbons (PAHs), and, first of all, benz(a)pyrene (BAP), have been reported by different authors and for different taxonomic groups(Kang et al, 2007;Liu, Pan, Jin, & Cai, 2015;Tamburro et al, 2004;Weisman, Alkio, & Colón-Carmona, 2010;Yang et al, 2014). Usually, only negative effects of PAHs including oxidative stress(Liu, Pan, et al, 2015;Weisman et al, 2010;Yang et al, 2014), imbalance of cellular metabolism and prevalence of catabolism over anabolic processes(Weisman et al, 2010), mutagenesis and cancerogenesis(Liu, Pan, et al, 2015;Yang et al, 2014), DNA damage(Yang et al, 2014) and cell death(Liu, Goa, et al, 2015;Weisman et al, 2010) are mentioned in the studies on multicellular organisms. We hypothesize that hydrocarbon-induced oxidative stress can be both detrimental for individual bacterial cells but beneficial for adaptation of the bacterial population.ROS formed during oxidative stress can play a role in microbiological transformation of hydrocarbons and accelerate bacterial evolution intensifying mutagenesis and the genome recombination.…”

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confidence: 64%

“…Various manifestations of oxidative stress in the presence of polyaromatic hydrocarbons (PAHs), and, first of all, benz(a)pyrene (BAP), have been reported by different authors and for different taxonomic groups (Kang et al, ; Liu, Pan, Jin, & Cai, ; Tamburro et al, ; Weisman, Alkio, & Colón‐Carmona, ; Yang et al, ). Usually, only negative effects of PAHs including oxidative stress (Liu, Pan, et al, ; Weisman et al, ; Yang et al, ), imbalance of cellular metabolism and prevalence of catabolism over anabolic processes (Weisman et al, ), mutagenesis and cancerogenesis (Liu, Pan, et al, ; Yang et al, ), DNA damage (Yang et al, ) and cell death (Liu, Goa, et al, ; Weisman et al, ) are mentioned in the studies on multicellular organisms. We hypothesize that hydrocarbon‐induced oxidative stress can be both detrimental for individual bacterial cells but beneficial for adaptation of the bacterial population.…”

Section: Discussionmentioning

confidence: 99%

Cyclohexane, naphthalene, and diesel fuel increase oxidative stress, CYP153, sodA, and recA gene expression in Rhodococcus erythropolis

et al. 2019

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In this study, we compared the expression of CYP153, sodA, sodC, and recA genes and ROS generation in hydrocarbon‐degrading Rhodococcus erythropolis in the presence of cyclohexane, naphthalene, and diesel fuel. The expression of cytochrome P450, sodA (encoding Fe/Mn superoxide dismutase), recA, and superoxide anion radical generation rate increased after the addition of all studied hydrocarbons. The peak of CYP153, sodA, and recA gene expression was registered in the presence of naphthalene. The same substrate upregulated the Cu/Zn superoxide dismutase gene, sodC. Cyclohexane generated the highest level of superoxide anion radical production. Hydrogen peroxide accumulated in the medium enriched with diesel fuel. Taken together, hydrocarbon biotransformation leads to oxidative stress and upregulation of antioxidant enzymes and CYP153 genes, and increases DNA reparation levels in R. erythropolis cells.

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Differential gene expression analysis of benzo(a)pyrene toxicity in the clam, Ruditapes philippinarum

Cited by 16 publications

References 59 publications

Ecotoxicological Effects of Chemical Contaminants Adsorbed to Microplastics in the Clam Scrobicularia plana

Ecotoxicological Effects of Chemical Contaminants Adsorbed to Microplastics in the Clam Scrobicularia plana

Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)

Cyclohexane, naphthalene, and diesel fuel increase oxidative stress, CYP153, sodA, and recA gene expression in Rhodococcus erythropolis

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