2010
DOI: 10.1186/1471-2164-11-582
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Differential gene expression during the moult cycle of Antarctic krill (Euphausia superba)

Abstract: BackgroundAll crustaceans periodically moult to renew their exoskeleton. In krill this involves partial digestion and resorption of the old exoskeleton and synthesis of new cuticle. Molecular events that underlie the moult cycle are poorly understood in calcifying crustaceans and even less so in non-calcifying organisms such as krill. To address this we constructed an Antarctic krill cDNA microarray in order to generate gene expression profiles across the moult cycle and identify possible activation pathways.R… Show more

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Cited by 49 publications
(55 citation statements)
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“…In the laboratory, the heads were taken for RNA extraction (Buchholz 1982) that were female but yet to mature fully (stage IIB or IIIA) (Makarov & Denys 1980). This step was carried out to avoid gene expression differences between sexes or moult stages from dominating the comparison (see Seear et al 2010). Altogether, 126 krill were examined, from which 37 krill suited the above criteria: 11 from Peninsula winter samples, 18 from Peninsula summer samples, and 8 from South Georgia winter samples.…”
Section: Krill Capture and Preservationmentioning
confidence: 99%
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“…In the laboratory, the heads were taken for RNA extraction (Buchholz 1982) that were female but yet to mature fully (stage IIB or IIIA) (Makarov & Denys 1980). This step was carried out to avoid gene expression differences between sexes or moult stages from dominating the comparison (see Seear et al 2010). Altogether, 126 krill were examined, from which 37 krill suited the above criteria: 11 from Peninsula winter samples, 18 from Peninsula summer samples, and 8 from South Georgia winter samples.…”
Section: Krill Capture and Preservationmentioning
confidence: 99%
“…This was later followed up by sequencing of a further 8 clones from the South Georgia winter cDNA library found to be differentially expressed in the microarray study. Sequencing was performed following procedures described in Seear et al (2010). Sequence chromatograms were processed using Geneious 5.3 (Drummond et al 2010) that trimmed vector, low quality sequence and 3'-polyadenylate tails.…”
Section: Sequencingmentioning
confidence: 99%
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