Differential gene expression in the mesocorticolimbic system of innately high- and low-impulsive rats

Moloney, Gerard M.; Oeffelen, Wesley E. P. A. van; Ryan, Feargal J.; Wouw, Marcel van de; Cowan, Caitlin S. M.; Claesson, Marcus J.; Schellekens, Harriët; Dinan, Timothy G.

doi:10.1016/j.bbr.2019.01.022

Cited by 11 publications

(11 citation statements)

References 51 publications

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“…The above findings seem to limit the possibility that inhibition of hippocampal Mal expression as shown in the present study is linked to Mal alterations in the nucleus accumbens [20]. Furthermore, downregulation in the hippocampal Mal expression (present study) is also not related to impulsivity-as this kind of behavior is a key component of CUD [21]-since increased accumbal Mal in highly impulsive rats was recently discovered [22].…”

Section: Discussioncontrasting

confidence: 46%

Cocaine Administration and Its Abstinence Conditions Modulate Neuroglia

Gawlińska

Frankowska

Gawliński

et al. 2020

IJMS

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Cocaine induces neuronal changes as well as non-neuronal (astrocytes, microglia, oligodendroglia) mechanisms, but these changes can also be modulated by various types of drug abstinence. Due to the very complex and still incompletely understood nature of cocaine use disorder, understanding of the mechanisms involved in addictive behavior is necessary to further search for effective pharmacotherapy of this disease. The aim of this study was to investigate changes at the gene and protein levels associated with glial cell activity after cocaine exposure, as well as during early cocaine abstinence (3 days) with extinction training or in home cage isolation. Cocaine self-administration significantly decreased myelin regulatory factor (MYRF) and cyclic nucleotide phosphodiesterase (CNP) expression in the hippocampus as well as pleckstrin (PLEK) and T-lymphocyte activation antigen (CD86) in the rat striatum. Depending on cocaine abstinence conditions, microglial PLEK expression was increased through extinction training but did not change in the home cage isolation. In addition, downregulation of gene expression associated with oligodendrocytes (CNP, MYRF) and microglia regulator of G protein signaling 1 (RGS1) was observed in the hippocampus, regardless of the type of drug abstinence, while downregulation of myelin and lymphocyte protein (MAL) expression was found only in rats exposed to abstinence in the home cage. Taken together, the presented results strongly suggest that cocaine abstinence evokes significant changes in gene expression associated with the proper functioning of glial cells, suggesting their significant involvement in adaptive changes in the brain associated with cocaine exposure. Interestingly, drug abstinence conditions are important factors influencing observed changes at the transcript levels of selected genes, which may be of clinical interest.

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Section: Discussioncontrasting

confidence: 46%

Cocaine Administration and Its Abstinence Conditions Modulate Neuroglia

Gawlińska

Frankowska

Gawliński

et al. 2020

IJMS

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“…Transcriptional differences between HI and LI rats have been found in the Nucleus Accumbens (NAc), ventral tegmental area, dorsomedial striatum and orbitofrontal cortex at basal conditions (Besson et al, 2013, Caprioli et al, 2014, Moloney et al, 2019). In the NAc, HI animals present a decreased availability of D1 and D2/3 receptors and of dopamine transporter (DAT) (Caprioli et al, 2015, Jupp et al, 2013) as well as a reduction in grey matter density (Caprioli et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

High trait impulsivity potentiates the effects of chronic pain on impulsive behavior

Cunha

Esteves

Pereira-Mendes

et al. 2020

Neurobiology of Pain

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“…Gene ontology (GO) (molecular function, 2021 database) was performed separately on upregulated and downregulated datasets (Hong et al, 2014) using EnrichR and the combined score metric as previously described; this is a product of the log of the p value computed by the Fisher exact test and the z score (Xie et al, 2021). DEU was performed using DEXSeq (Anders et al, 2012; Moloney et al, 2019). DEU was performed using DEXSeq (Anders et al, 2012; Moloney et al, 2019) As per the manual, DEXSeq is a strand‐specific analysis that measures the reads that map to a specific exon a gene and compares this with the amount of reads for other exons in that gene using the formula ‘number of transcripts from the gene that contain this exon divided by number of all transcripts from the gene’ (Anders et al, 2012).…”

Section: Methodsmentioning

confidence: 99%

“…DEU was performed using DEXSeq (Anders et al, 2012; Moloney et al, 2019). DEU was performed using DEXSeq (Anders et al, 2012; Moloney et al, 2019) As per the manual, DEXSeq is a strand‐specific analysis that measures the reads that map to a specific exon a gene and compares this with the amount of reads for other exons in that gene using the formula ‘number of transcripts from the gene that contain this exon divided by number of all transcripts from the gene’ (Anders et al, 2012).…”

Section: Methodsmentioning

confidence: 99%

The impact of psychosocial defeat stress on the bed nucleus of the stria terminalis transcriptome in adult male mice

Gururajan

Bastiaanssen

Silva

et al. 2021

Eur J of Neuroscience

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The bed nucleus of the stria terminalis (BNST) is a focal point for the convergence of inputs from canonical stress‐sensitive structures to fine‐tune the response to stress. However, its role in mediating phenotypes of stress resilience or susceptibility is yet to be fully defined. In this study, we carried out unbiased RNA‐sequencing to analyse the BNST transcriptomes of adult male mice, which were classified as resilient or susceptible following a 10‐day chronic psychosocial defeat stress paradigm. Pairwise comparisons revealed 20 differentially expressed genes in resilience (6) and susceptible (14) mice compared with controls. An in silico validation of our data against an earlier study revealed significant concordance in gene expression profiles associated with resilience to chronic stress. Enrichment analysis revealed that resilience is linked to functions including retinoic acid hydrolase activity, phospholipase inhibitor and tumour necrosis factor (TNF)‐receptor activities, whereas susceptibility is linked to alterations in amino acid transporter activity. We also identified differential usage of 134 exons across 103 genes associated with resilience and susceptibility; enrichment analysis for genes with differential exon usage in resilient mice was linked to functions including adrenergic receptor binding mice and oxysterol binding in susceptible mice. Our findings highlight the important and underappreciated role of the BNST in stress resilience and susceptibility and reveal research avenues for follow‐up investigations.

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Differential gene expression in the mesocorticolimbic system of innately high- and low-impulsive rats

Cited by 11 publications

References 51 publications

Cocaine Administration and Its Abstinence Conditions Modulate Neuroglia

Cocaine Administration and Its Abstinence Conditions Modulate Neuroglia

High trait impulsivity potentiates the effects of chronic pain on impulsive behavior

The impact of psychosocial defeat stress on the bed nucleus of the stria terminalis transcriptome in adult male mice

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