Differential gene expression of serine protease inhibitors in bovine ovarian follicle: possible involvement in follicular growth and atresia

Abstract

BackgroundSERPINs (serine protease inhibitors) regulate proteases involving fibrinolysis, coagulation, inflammation, cell mobility, cellular differentiation and apoptosis. This study aimed to investigate differentially expressed genes of members of the SERPIN superfamily between healthy and atretic follicles using a combination of microarray and quantitative real-time PCR (QPCR) analysis. In addition, we further determined mRNA and protein localization of identified SERPINs in estradiol (E2)-active and E2-inactive follicles by in situ hybridization and immunohistochemistry.MethodsWe performed microarray analysis of healthy (10.7 +/- 0.7 mm) and atretic (7.8 +/- 0.2 mm) follicles using a custom-made bovine oligonucleotide microarray to screen differentially expressed genes encoding SERPIN superfamily members between groups. The expression profiles of six identified SERPIN genes were further confirmed by QPCR analysis. In addition, mRNA and protein localization of four SERPINs was investigated in E2-active and E2-inactive follicles using in situ hybridization and immunohistochemistry.ResultsWe have identified 11 SERPIN genes expressed in healthy and atretic follicles by microarray analysis. QPCR analysis confirmed that mRNA expression of four SERPINs (SERPINA5, SERPINB6, SERPINE2 and SERPINF2) was greater in healthy than in atretic follicles, while two SERPINs (SERPINE1 and SERPING1) had greater expression in atretic than in healthy follicles. In situ hybridization showed that SERPINA5, SERPINB6 and SERPINF2 mRNA were localized in GCs of E2-active follicles and weakly expressed in GCs of E2-inactive follicles. SERPING1 mRNA was localized in both GCs and the theca layer (TL) of E2-inactive follicles and a weak hybridization signal was also detected in both GCs and TL of E2-active follicles. Immunohistochemistry showed that SERPINA5, SERPINB6 and SERPINF2 were detected in GCs of E2-active and E2-inactive follicles. SERPING1 protein was localized in both GCs and the TL of E2-active and E2-inactive follicles.ConclusionsOur results demonstrate a characteristic expression of SERPIN superfamily member genes in bovine healthy and atretic follicles. The cell-type-and stage-specific expression of SERPINs may be associated with bovine follicular growth and atresia.

Comprehensive analysis of mRNAs and miRNAs in the ovarian follicles of uniparous and multiple goats at estrus phase

“…2e, f). Approximately 37% of the DEgenes in the Uni-S vs Mul-S group and 41% in the Uni-L vs Mul-L group were reported to be associated with mammalian reproduction, such as TNFAIP6, MMP9, INSL3, LEPR, 3BHSD, LHCGR, ARL4C, CD36, CYP11A1, AMDHD1, SPOCK2, AMDHD1, MFAP5, CCL21, PTGFR, and SERPINA5 [23,[47][48][49][50][51][52][53][54][55][56][57][58][59][60]. Of these genes, TNFAIP6, CYP11A1, CD36, PTGFR, and SER-PINA5 were found to be associated with the ovulation rate.…”

“…In the GCs of periovulatory follicles of mice, the expression of PTGFR was shown to be drastically reduced [75].. Our results showed that PTGFR expression was downregulated in the large follicle from multiple goats, suggesting that the greater reduction of PTGFR expression observed in multiple goats may contribute to a the higher ovulation rate. Previous studies have reported that SERPINA5, a protease inhibitor, was expressed in the reproductive tract of adult mice and in the GCs of bovine follicles and that was highly expressed in bovine healthy follicles [60,76]. SERPINA5 expression was shown to be downregulated in ovarian cancer (OC) [77][78][79].…”

Identification of Significant Genes with poor prognosis in Liver Metastasis of Colorectal Cancer via Bioinformatical Analysis

“…However, the mechanisms and effects of complement speci c deregulation on tumor microenvironment are not clear. More works are needed to elucidate the complex process of cancer cells controlling complement activation and how complement affects tumor progression SERPING1 is also known as C1IH or CINH SERPING1 belongs to the serpins family of serine protease inhibitors, which regulate the proteases involved in brinolysis, coagulation, in ammation, cell migration, cell differentiation and apoptosis (Hayashi, Ushizawa et al 2011). Many studies have reported that the SERPING1 expression is up-regulated in tumors, including glioblastoma (Fornvik, Maddahi et al 2017), breast cancer and gastric cancer (Wojtukiewicz 1998).…”

Comprehensive Analysis of mRNAs and miRNAs in the Ovarian Follicles of Uniparous and Multiple Goats at Estrus Phase

“…Our result showed that PTGFR gene was downregulated in large follicles from multiple goats, suggesting that the greater reduction of PTGFR gene expression in multiple goats may contribute to ovulate more. The previous studies reported that SERPINA5, as a protease inhibitor, was expressed in the reproductive tract of adult mice and in the GCs of bovine follicles, and was highly expressed in bovine healthy follicles [54,67]. SERPINA5 gene was down-regulated in ovarian cancer (OC) studies [68][69][70].…”