Differential gene expression profiles of whole lesions from patients with oral lichen planus

Tao, Xiaoan; Li, Chunyang; Xia, Juan; Yang, Xi; Xiao-hua, Chen; Jian, Yutao; Cheng, Bin

doi:10.1111/j.1600-0714.2009.00764.x

Cited by 28 publications

(20 citation statements)

References 32 publications

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“…Since immunity and inflammation are involved in the development of OLP (Reichart and Philipsen 2005), we estimated that EIF1AY, WFDC12, and CXCL13 might participate in OLP through regulating inflammation and immunity. In the present study, CXCL13 BPIFC DEGs differentially expressed genes, GO gene ontology, ID identifier, BP biological process, CC cellular component was a hub gene that closely interacted with eight genes in PPI network, and the expression of CXCL13 was significantly up-regulated, coinciding with previous studies (Tao et al 2009). Therefore, it's suspected that CXCL13 might play a critical role in OLP.…”

Section: Discussionsupporting

confidence: 91%

Genes involved in keratinization, keratinocyte and epithelium differentiation are aberrantly regulated in oral lichen planus

Liu

Wang

et al. 2015

Genes Genom

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Oral lichen planus (OLP) is a common inflammatory oral mucosal disease and a potential premalignant status of oral squamous cell carcinoma. This study aims to explore the molecular mechanisms of OLP. The gene expression dataset (GSE38616) of OLP tissues and healthy controls was downloaded from Gene Expression Omnibus database. After preprocessing the raw data through affy package, limma package was utilized to identify differentially expressed genes (DEGs) (criteria: p B 0.05 and |log 2 fold change| C 2). Then, functional and pathway enrichment analyses were performed by using DAVID software (criterion: p value \0.1). Besides, STRING sever was utilized to investigate protein-protein interactions (PPIs) based on which PPI network was constructed (criterion: combined score [0.4). Finally, the transcription factor binding sites (TFBSs) of DEGs were predicted through WGRV software (criterion: p value \0.0001). A total of ten DEGs were identified, including two down-regulated and eight up-regulated DEGs, which were enriched in nine functions mainly about keratinization, differentiation and development of keratinocyte and epithelium. Besides, PPI network was constructed, and CXCL13 was a hub gene. Furthermore, four conserved TFBSs (AR, dlx3, ALX-3, and Msx-1) were co-existed in SLC6A14, CXCL13, and CDSN. CDSN, LCE3D, LCE3E, and SPRR2B might play a role in OLP through participating in keratinization, differentiation and development of keratinocyte and epithelium, while EIF1AY, WFDC12, and CXCL13 might participate in OLP through regulating inflammation and immunity. These predictions might promote the understanding of OLP mechanism. However, further studies are required to validate the bioinformatics outcomes.

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Section: Discussionsupporting

confidence: 91%

Genes involved in keratinization, keratinocyte and epithelium differentiation are aberrantly regulated in oral lichen planus

Liu

Wang

et al. 2015

Genes Genom

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“…Increases in certain cytokines and receptors in immune cells via TLR activation can have direct effects on T cell response in autoimmune diseases. 12,16 Some years ago Xao et al published the only global gene expression analysis of tissue from this disease 17 and we have tried to use this as a starting point to discern genes differentially expressed in the epithelium of patients with OLP or OLR. Various factors have been shown to be associated with OLP based on changes in protein or RNA levels in OLP mucosal tissue or even protein levels in saliva.…”

Section: Introductionmentioning

confidence: 99%

Gene expression based evidence of innate immune response activation in the epithelium with oral lichen planus

Adami

Yeung

Stucki

et al. 2014

Archives of Oral Biology

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Objective Oral lichen planus (OLP) is a disease of the oral mucosa of unknown cause producing lesions with an intense band-like inflammatory infiltrate of T cells to the subepithelium and keratinocyte cell death. We performed gene expression analysis of the oral epithelium of lesions in subjects with OLP and its sister disease, oral lichenoid reaction (OLR), in order to better understand the role of the keratinocytes in these diseases. Design Fourteen patients with OLP or OLR were included in the study, along with a control group of 23 subjects with a variety of oral diseases and a normal group of 17 subjects with no clinically visible mucosal abnormalities. Various proteins have been associated with OLP, based on detection of secreted proteins or changes in RNA levels in tissue samples consisting of epithelium, stroma, and immune cells. The mRNA level of twelve of these genes expressed in the epithelium was tested in the three groups. Results Four genes showed increased expression in the epithelium of OLP patients: CD14, CXCL1, IL8, and TLR1, and at least two of these proteins, TLR1 and CXCL1, were expressed at substantial levels in oral keratinocytes. Conclusions Because of the large accumulation of T cells in lesions of OLP it has long been thought to be an adaptive immunity malfunction. We provide evidence that there is increased expression of innate immune genes in the epithelium with this illness, suggesting a role for this process in the disease and a possible target for treatment.

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“…The histopathology of OLP reveals saw-tooth-shaped elongation of the rete ridges. 3,6 Since epidermal growth factor receptor (EGFR) is critical for the proliferation of keratinocytes, it is possible that EGFR overexpression is involved in the process of carcinogenesis in oral epithelium. 5 The protooncogene EGFR, also known as ERBB, is a welldocumented tyrosine kinase growth factor receptor.…”

Section: Introductionmentioning

confidence: 99%

Molecular Targeting of Her-2/neu Protein Is Not Recommended as an Adjuvant Therapy in Oral Squamous Cell Carcinoma and Oral Lichen Planus

et al. 2015

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Differential gene expression profiles of whole lesions from patients with oral lichen planus

Cited by 28 publications

References 32 publications

Genes involved in keratinization, keratinocyte and epithelium differentiation are aberrantly regulated in oral lichen planus

Genes involved in keratinization, keratinocyte and epithelium differentiation are aberrantly regulated in oral lichen planus

Gene expression based evidence of innate immune response activation in the epithelium with oral lichen planus

Molecular Targeting of Her-2/neu Protein Is Not Recommended as an Adjuvant Therapy in Oral Squamous Cell Carcinoma and Oral Lichen Planus

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