Differential gene expression profiling analysis in Pleurotus ostreatus during interspecific antagonistic interactions with Dichomitus squalens and Trametes versicolor

Zhong, Zixuan; Li, Liu; Chang, Peng; Xie, Hua; Zhang, Huiting; Igarashi, Yasuo; Li, Nannan; Luo, Feng

doi:10.1016/j.funbio.2017.08.008

Cited by 11 publications

(6 citation statements)

References 51 publications

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“…After quantification by TBS380, the paired-end RNA-seq sequencing library was sequenced with the Illumina HiSeq X Ten (2 × 150 bp read length). 23 The raw paired end reads were trimmed and qualitycontrolled by SeqPrep and Sickle with default parameters. Then, clean reads were separately aligned to the reference genome with orientation mode using TopHat software.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Geniposide Improves Glucose Homeostasis via Regulating FoxO1/PDK4 in Skeletal Muscle

Yan

Pan

Zhang

et al. 2019

J. Agric. Food Chem.

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It is well-known that imbalance state of glucose metabolism triggers many metabolic diseases and glucose uptake in skeletal muscle accounts for 90% of body weight. Geniposide is one of the major natural bioactive constituents of gardenia fruit, and the regulation of geniposide on glucose metabolism in skeletal muscle has not yet been investigated. Here, on the basis of microarray analysis, we discovered that geinposide decreased pyruvate dehydrogenase kinase 4 (PDK4) expression in skeletal muscle of mice and subsequently found that geniposide inhibited the expressions of forkhead box O1 (FoxO1), PDK4, and phosphorylated pyruvate dehydrogenase in vitro and in vivo. Moreover, geniposide promoted a switch of slow-to-fast myofiber type and glucose utilization, suggesting that geniposide improved glucose homeostasis. In addition, mechanistic studies revealed that geniposide played above roles by regulating FoxO1/PDK4, which controlled fuel selection via pyruvate dehydrogenase. Meanwhile, effects of geniposide mentioned above could be reversed by FoxO1 overexpression. Together, these results establish that geniposide confers controls on fuel usage and glucose homeostasis through FoxO1/PDK4 in skeletal muscle.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

Geniposide Improves Glucose Homeostasis via Regulating FoxO1/PDK4 in Skeletal Muscle

Yan

Pan

Zhang

et al. 2019

J. Agric. Food Chem.

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“…Aryl alcohols function in harmony with ligninolytic enzymes by providing H2O2 to the peroxidases and are even synthesized de novo in some WRF (Hernández-Ortega et al, 2012). Ssp1 was also the most upregulated gene, up to 700fold, during interspecific interactions between P. ostreatus with Dichomitus squalen or Trametes versicolor (Zhong et al, 2017).…”

Section: Ssp1 In P Ostreatus -A Case Studymentioning

confidence: 99%

Seeking the Roles for Fungal Small-Secreted Proteins in Affecting Saprophytic Lifestyles

2020

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Small secreted proteins (SSPs) comprise 40-60% of the total fungal secretome and are present in fungi of all phylogenetic groups, representing the entire spectrum of lifestyles. They are characteristically shorter than 300 amino acids in length and have a signal peptide. The majority of SSPs are coded by orphan genes, which lack known domains or similarities to known protein sequences. Effectors are a group of SSPs that have been investigated extensively in fungi that interact with living hosts, either pathogens or mutualistic systems. They are involved in suppressing the host defense response and altering its physiology. Here, we aim to delineate some of the potential roles of SSPs in saprotrophic fungi, that have been bioinformatically predicted as effectors, and termed in this mini-review as "effector-like" proteins. The effector-like Ssp1 from the white-rot fungus Pleurotus ostreatus is presented as a case study, and its potential role in regulating the ligninolytic system, secondary metabolism, development, and fruiting body initiation are discussed. We propose that deciphering the nature of effectorlike SSPs will contribute to our understanding of development and communication in saprophytic fungi, as well as help, to elucidate the origin, regulation, and mechanisms of fungal-host, fungal-fungal, and fungal-bacterial interactions.

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“…Overall, the results suggest that Ssp1 is involved in morphogenesis during fruiting body initiation. Studies by Zhong et al and Luo et al (31,32) focused on analysis of differentially expressed genes and metabolites in P. ostreatus under an interspecific antagonistic interaction with the WRF Dichomitus squalens and Trametes versicolor. In their studies, they mentioned PLEOSDRAFT_156192, a hypothetical protein that is encoded by the most highly upregulated gene during the interaction between both fungi.…”

Section: Figmentioning

confidence: 99%

“…Homologues of Ssp1 are present in at least 22 other fungi, many of which are wood decay or mycorrhizal fungi (16). The fact that some of them have already been reported to have a role in interspecies antagonistic interactions (31) indicates the importance of Ssp1 and Ssp1like proteins for fungi in their natural environment.…”

Section: Figmentioning

confidence: 99%

Manipulating the Expression of Small Secreted Protein 1 (Ssp1) Alters Patterns of Development and Metabolism in the White-Rot Fungus Pleurotus ostreatus

Feldman

Amedi

Carmeli

et al. 2019

Appl Environ Microbiol

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The function of small secreted proteins (SSPs) in saprotrophic fungi is, for the most part, unknown. The white-rot mushroom Pleurotus ostreatus produces considerable amounts of SSPs at the onset of secondary metabolism, during colony development, and in response to chemical compounds such as 5-hydroxymethylfurfural and aryl alcohols. Genetic manipulation of Ssp1, by knockdown (KDssp1) or overexpression (OEssp1), indicated that they are, in fact, involved in the regulation of the ligninolytic system. To elucidate their potential involvement in fungal development, quantitative secretome analysis was performed during the trophophase and the idiophase and at a transition point between the two growth phases. The mutations conferred a time shift in the secretion and expression patterns: OEssp1 preceded the entrance to idiophase and secondary metabolism, while KDssp1 was delayed. This was also correlated with expression patterns of selected genes. The KDssp1 colony aged at a slower pace, accompanied by a slower decline in biomass over time. In contrast, the OEssp1 strain exhibited severe lysis and aging of the colony at the same time point. These phenomena were accompanied by variations in yellow pigment production, characteristic of entrance of the wild type into idiophase. The pigment was produced earlier and in a larger amount in the OEssp1 strain and was absent from the KDssp1 strain. Furthermore, the dikaryon harboring OEssp1 exhibited a delay in the initiation of fruiting body formation as well as earlier aging. We propose that Ssp1 might function as a part of the fungal communication network and regulate the pattern of fungal development and metabolism in P. ostreatus. IMPORTANCE Small secreted proteins (SSPs) are common in fungal saprotrophs, but their roles remain elusive. As such, they comprise part of a gene pool which may be involved in governing fungal lifestyles not limited to symbiosis and pathogenicity, in which they are commonly referred to as "effectors." We propose that Ssp1 in the white-rot fungus Pleurotus ostreatus regulates the transition from primary to secondary metabolism, development, aging, and fruiting body initiation. Our observations uncover a novel regulatory role of effector-like SSPs in a saprotroph, suggesting that they may act in fungal communication as well as in response to environmental cues. The presence of Ssp1 homologues in other fungal species supports a common potential role in environmental sensing and fungal development.

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Differential gene expression profiling analysis in Pleurotus ostreatus during interspecific antagonistic interactions with Dichomitus squalens and Trametes versicolor

Cited by 11 publications

References 51 publications

Geniposide Improves Glucose Homeostasis via Regulating FoxO1/PDK4 in Skeletal Muscle

Geniposide Improves Glucose Homeostasis via Regulating FoxO1/PDK4 in Skeletal Muscle

Seeking the Roles for Fungal Small-Secreted Proteins in Affecting Saprophytic Lifestyles

Manipulating the Expression of Small Secreted Protein 1 (Ssp1) Alters Patterns of Development and Metabolism in the White-Rot Fungus Pleurotus ostreatus

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