Differential GFP Expression Patterns Induced by Different Heavy Metals in Tg(hsp70:gfp) Transgenic Medaka (Oryzias latipes)

Ng, Grace Hwee Boon; Xu, Hongyan; Pi, Na; Kelly, Barry C.; Gong, Zhiyuan

doi:10.1007/s10126-015-9620-5

Cited by 10 publications

(2 citation statements)

References 32 publications

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“…To detect heavy metals in the water, heavy metal monitoring medaka have been reported. The GFP in the transgenic medaka is driven by Hsp70, which responds to 110 nM Cd 2+ after 24 h exposure 30 . D. magna MetalloG can respond to 18 nM Cd 2+ after 24 h; therefore, the Daphnia biosensor is more sensitive than the medaka.…”

Section: Resultsmentioning

confidence: 99%

Production of genome-edited Daphnia for heavy metal detection by fluorescence

Arao

Kato

Nong

et al. 2020

Sci Rep

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Aquatic heavy metal pollution is a growing concern. To facilitate heavy metal monitoring in water, we developed transgenic Daphnia that are highly sensitive to heavy metals and respond to them rapidly. Metallothionein A, which was a metal response gene, and its promoter region was obtained from Daphnia magna. A chimeric gene fusing the promoter region with a green fluorescent protein (GFP) gene was integrated into D. magna using the TALEN technique and transgenic Daphnia named D. magna MetalloG were produced. When D. magna MetalloG was exposed to heavy metal solutions for 1 h, GFP expression was induced only in their midgut and hepatopancreas. The lowest concentrations of heavy metals that activated GFP expression were 1.2 µM Zn2+, 130 nM Cu2+, and 70 nM Cd2+. Heavy metal exposure for 24 h could lower the thresholds even further. D. magna MetalloG facilitates aqueous heavy metal detection and might enhance water quality monitoring.

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Section: Resultsmentioning

confidence: 99%

Production of genome-edited Daphnia for heavy metal detection by fluorescence

Arao

Kato

Nong

et al. 2020

Sci Rep

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“…Transient knockdown of gene expression through the injection of Morpholino antisense oligos has also been a suitable approach for studying mechanisms of osteotoxicity, particular at early developmental stages (Blum et al, 2015). Various transgenic fish lines have been developed to study the toxic effects of compounds such as nanoparticles (Pan et al, 2013), heavy metals (Ng et al, 2015), bisphosphonates (Yu et al, 2016) or organic chemicals acting through nuclear receptors (NRs; Terrien et al, 2011;Gorelick and Halpern, 2011;Benato et al, 2014). Although their potential is considerable, they show a great variability on their sensitivity, and only very few are able to detect responses to environmentally relevant exposures (Lee et al, 2014).…”

Section: Fish Systems For Osteotoxicitymentioning

confidence: 99%

Fish as a model to assess chemical toxicity in bone

et al. 2018

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Environmental toxicology has been expanding as growing concerns on the impact of produced and released chemical compounds over the environment and human health are being demonstrated. Among the toxic effects observed in organisms exposed to pollutants, those affecting skeletal tissues (osteotoxicity) have been somehow overlooked in comparison to hepato-, immune-, neuro- and/or reproductive toxicities. Nevertheless, sub-lethal effects of toxicants on skeletal development and/or bone maintenance may result in impaired growth, reduced survival rate, increased disease susceptibility and diminished welfare. Osteotoxicity may occur by acute or chronic exposure to different environmental insults. Because of biologically and technically advantagous features - easy to breed and inexpensive to maintain, external and rapid rate of development, translucent larvae and the availability of molecular and genetic tools - the zebrafish (Danio rerio) has emerged in the last decade as a vertebrate model system of choice to evaluate osteotoxicity. Different experimental approaches in fish species and analytical tools have been applied, from in vitro to in vivo systems, from specific to high throughput methodologies. Current knowledge on osteotoxicity and underlying mechanisms gained using fish, with a special emphasis on zebrafish systems, is reviewed here. Osteotoxicants have been classified into four categories according to the pathway involved in the transduction of the osteotoxic effects: activation/inhibition of membrane and/or nuclear receptors, alteration of redox condition, mimicking of bone constituents and unknown pathways. Knowledge on these pathways is also reported here as it may provide critical insights into the development, production and release of future chemical compounds with none or low osteotoxicity, thus promoting the green/environmental friendly chemistry.

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