Differential identification of GSH for acute coronary syndrome using a colorimetric sensor based on nanoflower-like artificial nanozymes

Zhang, Dandan; Zhang, Hongjin; Sun, He; Yang, Yuanzhen; Wang, Zhong; Chen, Qing; Ren, Qunxiang; Jin, Ge; Zhang, Yang

doi:10.1016/j.talanta.2023.124967

Cited by 6 publications

(1 citation statement)

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“…In recent years, a number of analytical strategies such as capillary electrophoresis, electrochemistry, fluorometry, colorimetry, and surface-enhanced Raman scattering (SERS) have been proposed and exploited for biothiol detection. Especially, optical sensing platforms have sparked widespread discussion owing to their valuable features, including exceptional stability, simple operation, and fast response .…”

Section: Introductionmentioning

confidence: 99%

Nanozyme-Inhibited SERS Multichannel Paper-Based Sensor Array for the Quantification and Identification of Biothiols and Cancer Cells Based on Three Ag-Based Nanomaterials

Wang,

Chen,

et al. 2024

Anal. Chem.

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Biothiols play essential roles in maintaining normal physiological functions, resisting oxidative stress, and protecting cell health. Establishing an effective and reliable sensor array for the accurate quantification and discrimination of diverse biothiols is extremely meaningful. In this work, Ag/ Mn 3 O 4 , Ag 3 PO 4 , and Ag 3 Cit with excellent oxidase-mimetic activity and surfaceenhanced Raman scattering (SERS)-enhanced features have been prepared and loaded onto Whatman filter paper (WFP) to build SERS paper chips as three sensing channels, which can induce 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation to SERS-active reporters (TMBox) and concurrently generate prominent SERS signals. Nevertheless, the addition of biothiols can suppress conversion from TMB to TMBox, which can cause the reduction of the SERS signal from TMBox. Interestingly, each SERS sensing channel can generate different TMBox signals' variations due to differences in the oxidative inhibition abilities of diverse biothiols and exclusive properties of each paper chip, which can be plotted as specific fingerprint patterns of each biothiol and further translated into intuitive two-dimensional (2D) clustering profiles through linear discriminant analysis (LDA) and hierarchical cluster analysis (HCA) techniques for precise identification of these six biothiols with the minimum concentration of 1 μM. More importantly, this SERS sensor array is exploited for the precise quantification of intracellular glutathione (GSH), and can differentiate between normal and cancer cells based on different intracellular GSH contents and even identify different types of tumor cells, demonstrating its powerful application prospects in disease diagnosis.

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