Differential immunohistochemical expression of JSRV capsid antigen and tumour biomarkers in classical and atypical OPA: a comparative study

Mishra, Santosh Kumar; Kumar, Pawan; Dar, Javeed Ahmad; George, Neethu; Singh, Vidya; Singh, Rajendra

doi:10.1080/09291016.2019.1610857

Cited by 4 publications

(10 citation statements)

References 24 publications

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“…IHC is also a useful method to identify small pulmonary lesions of OPA and to differentiate primary form metastatic pulmonary tumors, but is not routinely used as commercial test in veterinary practice [17]. IHC was recently used for evaluation of classical and atypical form of OPA in sheep [16,51].…”

Section: Discussionmentioning

confidence: 99%

“…The atypical OPA is less contagious than classical form [4]. The atypical OPA has been reported in Spain, Peru, Iran and India [4,12,16,25], but there is no evidence of its occurrence in other countries where OPA is commonly found.…”

Section: Introductionmentioning

confidence: 91%

“…There are two recognized forms of OPA which show gross, histological and immunohistochemical differences [4,12,16]. The lesions in classical OPA predominantly affect all pulmonary lobes and are located in the cranioventral area.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, intravitam diagnosis of exJSRV infection by PCR from blood offers many false negative results probably because the numbers of infected white blood cells is very low [26,27]. Additionally, the viral genetic material or proteins can also be detected by PCR and immunohistochemistry (IHC) respectively, in pulmonary and lymphoid tissues [16,17,28].…”

Section: Introductionmentioning

confidence: 99%

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Exogenous Jaagsiekte Sheep Retrovirus type 2 (exJSRV2) related to ovine pulmonary adenocarcinoma (OPA) in Romania: prevalence, anatomical forms, pathological description, immunophenotyping and virus identification

et al. 2020

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Background: Ovine pulmonary adenocarcinoma (OPA) is a neoplastic disease caused by exogenous Jaagsiekte Sheep Retrovirus (exJSRV). The prevalence of JSRV-related OPA in Eastern European countries, including Romania is unknown. We aimed to investigate: the prevalence and morphological features of OPA (classical and atypical forms) in the Transylvania region (Romania), the immunophenotype of the pulmonary tumors and their relationships with exJSRV infection. A total of 2693 adult ewes slaughtered between 2017 and 2019 in two private slaughterhouses from Transylvania region (Romania) was evaluated. Lung tumors were subsequently assessed by cytology, histology, immunocytochemistry, immunohistochemistry, electron microscopy and DNA testing.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Exogenous Jaagsiekte Sheep Retrovirus type 2 (exJSRV2) related to ovine pulmonary adenocarcinoma (OPA) in Romania: prevalence, anatomical forms, pathological description, immunophenotyping and virus identification

et al. 2020

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“…Additionally, these methods can be used as follow-up tests to confirm an infection in unclear cases. Typical lesions consist of neoplastic transformation and proliferation of type II pneumocytes and bronchial epithelial cells, with rare metastasis to the regional lymph nodes and further organs [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

A Combined Approach for Detection of Ovine Small Ruminant Retrovirus Co-Infections

Rosato

Abril

Hilbe

et al. 2023

Viruses

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Jaagsiekte retrovirus (JSRV)-induced ovine pulmonary adenocarcinoma (OPA) is an important ovine respiratory disease in Switzerland. Furthermore, ovine lungs with OPA frequently exhibited lesions suggestive of maedi-visna virus (MVV) or caprine arthritis encephalitis virus (CAEV) infection, indicating that co-morbidities might occur. Lungs and pulmonary lymph nodes were sampled from suspected OPA cases, inflammatory lung lesions and control lungs (total of 110 cases). Tissues were (a) processed for histology and immunohistochemistry (IHC), and (b) underwent DNA extraction and real-time PCR for JSRV, MVV and CAEV. Peptide sequences were used to generate virus-specific customized polyclonal antibodies. PCR-positive OPA cases and formalin-fixed and paraffin-embedded MVV- and CAEV-infected synovial cell pellets served as positive controls. Fifty-two lungs were histologically diagnosed with OPA. Histological evidence of MVV/CAEV infection was detected in 25 lungs. JSRV was detected by PCR in 84% of the suspected OPA cases; six were co-infected with MVV and one with CAEV. MVV was detected by PCR in 14 cases, and four lungs were positive for CAEV. Three lungs had MVV/CAEV co-infection. In IHC, JSRV was detected in 91% of the PCR-positive cases, whereas MVV and CAEV immunoreactivity was seen in all PCR-positive lungs. Although PCR showed a higher sensitivity compared to IHC, the combined approach allows for investigations on viral cell tropism and pathogenic processes in co-morbidities, including their potential interdependency. Furthermore, an immunohistochemical tool for specific differentiation of MVV and/or CAEV infection was implemented.

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Ovine pulmonary adenocarcinoma (OPA) in sheep: an update on epidemiology, pathogenesis and diagnosis

B N,

K N

et al. 2023

J Exp Bio & Ag Sci

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Ovine pulmonary adenocarcinoma (OPA) is a spontaneous lung tumor in sheep caused by Jaagsiekte sheep retrovirus (JSRV) belonging to the Retroviridae. The primary aim of this review work is to give brief insights into the epidemiological aspects of OPA based on a meta-analysis of available research work. This review article also discussed pathogenesis, diagnostic tests and control strategies available for OPA in Sheep. This will help in developing future strategies for disease-free status in India. This disease is endemic in Europe, Africa, Asia, and American continents, causing significant economic losses due to chronic respiratory illness and persistent infections in flocks. The virus is unique among retroviruses with selective affinity to lungs and is the only virus known to cause spontaneous lung tumors in sheep. The incubation time ranges for sheep with naturally occurring OPA ranged from one to four years. There are two pathological forms of the disease: classical and atypical. At an early stage, OPA is difficult to detect in sheep due to a lack of preclinical diagnostic methods, as JSRV is poorly immunogenic and doesn't induce an immune response. PCR, histopathology, and immunohistochemistry are recommended methods for OIE diagnosis. To become a JSRV-free country, mandatory surveillance, detection, and removal of positive animals are required, as OPA is difficult to control due to a lack of vaccines and preclinical diagnostic tests. Due to its similar histological and molecular pathogenesis to that of human lung cancer, OPA is considered an ideal large animal model of human lung adenocarcinoma.

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Differential immunohistochemical expression of JSRV capsid antigen and tumour biomarkers in classical and atypical OPA: a comparative study

Cited by 4 publications

References 24 publications

Exogenous Jaagsiekte Sheep Retrovirus type 2 (exJSRV2) related to ovine pulmonary adenocarcinoma (OPA) in Romania: prevalence, anatomical forms, pathological description, immunophenotyping and virus identification

Exogenous Jaagsiekte Sheep Retrovirus type 2 (exJSRV2) related to ovine pulmonary adenocarcinoma (OPA) in Romania: prevalence, anatomical forms, pathological description, immunophenotyping and virus identification

A Combined Approach for Detection of Ovine Small Ruminant Retrovirus Co-Infections

Ovine pulmonary adenocarcinoma (OPA) in sheep: an update on epidemiology, pathogenesis and diagnosis

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