Differential immunolocalization of estrogen receptor alpha and beta in rat ovary and uterus

Hiroi, Hisahiko; Inoue, Satoshi; Watanabe, Tôru; Goto, Wakako; Orimo, Akira; Momoeda, Mikio; Tsutsumi, Osamu; Taketani, Yuji; Muramatsu, Masami

doi:10.1677/jme.0.0220037

Cited by 135 publications

(105 citation statements)

References 44 publications

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“…For example, bone predominantly expresses ERα, except in the marrow which is primarily ERβ [63]. Similarly, in the uterus, ERα is greater but ERβ is expressed in the glandular epithelium [57,67]. A point of particular clinical relevance is that ERα is the target of E 2 's trophic effects in mammary and uterine tissue [63].…”

Section: Erβ-mediated Actions In the Hippocampus For E 2 'S Functionamentioning

confidence: 99%

Rapid and estrogen receptor beta mediated actions in the hippocampus mediate some functional effects of estrogen

Walf

Frye

2008

Steroids

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The steroid hormone, estradiol (E 2 ), has numerous targets in the central nervous system, including the hippocampus, which plays a key role in cognition and affective behavior. This review focuses on our evidence from studies in rodents that E 2 has diverse mechanisms in the hippocampus for its functional effects E 2 has rapid, membrane-mediated effects in the hippocampus to enhance cognitive performance. Administration of E 2 to the hippocampus of rats for 10 minutes following training enhances performance in a hippocampus-mediated task. Increased cell firing in the hippocampus occurs within this short time frame. Furthermore, administration of free E 2 or an E 2 conjugate, E 2 :bovine serum albumin (BSA), to the hippocampus produces similar performance-enhancing effects in this task, suggesting that E 2 has membrane actions in the hippocampus for these effects. Further evidence that E 2 has rapid, membrane-mediated effects is that co-administration of E 2 and inhibitors of mitogen activated protein kinase (MAPK), rather than intracellular E 2 receptors (ERs) or protein synthesis, attenuate the enhancing effects of E 2 in this task. Despite these data that demonstrate E 2 can have rapid and/or membrane-mediated effects in the hippocampus, there is clear evidence to suggest that intracellular ERs, particularly the β (rather than α) isoform of ERs, may be important targets for E 2 's functional effects for hippocampal processes. Administration of ligands that are specific for ERβ, but not ERα, have enhancing effects on hippocampal processes similar to that of E 2 (which has similar affinity for ERα and ERβ). These effects are attenuated when ERβ expression is knocked down in transgenic models or with central administration of antisense oligonucleotides. Thus, there may be a convergence of E 2 's actions through rapid, membranemediated effects and intracellular ERs and in the hippocampus for these functional effects.

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Section: Erβ-mediated Actions In the Hippocampus For E 2 'S Functionamentioning

confidence: 99%

Rapid and estrogen receptor beta mediated actions in the hippocampus mediate some functional effects of estrogen

Walf

Frye

2008

Steroids

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“…These findings are very similar to previous findings (Fitzpatrick et al 1999, Sar & Welsch 1999 indicating that, by immunocytochemistry, ER but not ER could be detected in the oviduct and uterus. Hiroi et al (1999) have reported that, in the rat uterus, the nuclei of glandular and luminal epithelial cells were also immunostained with ER antibodies and that only the nuclei of glandular epithelium cells were stained with anti-ER . Since RT-PCR analysis has shown low expression of ER mRNA in the rat uterus , it is possible that the approaches used in the present study were not sensitive enough to detect low amounts of ER at the cellular level.…”

Section: Female Reproductive Organsmentioning

confidence: 99%

Localization of oestrogen receptor alpha, oestrogen receptor beta and androgen receptors in the rat reproductive organs

Pelletier

Labrie

Labrie³

2000

Journal of Endocrinology

394

232

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There is now evidence that oestrogens and androgens can influence male and female reproductive systems. In order to accurately identify the sites of action of oestrogens and androgens, we have proceeded to the histological localization of the two oestrogen receptor (ER) subtypes, ER and ER , and the androgen receptor (AR) in the reproductive tissues of adult rats of both sexes. AR was detected by immunocytochemistry, while ER and ER were localized by both immunocytochemistry and in situ hybridization. In the pituitary gland of animals of both sexes, ER was found in the majority of nuclei of secretory cells in the anterior pituitary. The intermediate and posterior lobes did not show any staining. ER was not found to be expressed in any of the pituitary lobes. Using AR antibodies, nuclear staining was detected in about 50% of secretory cells of the anterior lobe, the intermediate and posterior lobes being completely unstained. In the testis, ER was localized in nuclei of Leydig cells as well as in round spermatocytes and spermatids, while ER could only be detected in Sertoli cell nuclei. AR immunoreactivity was found in nuclei of Sertoli, peritubular myoid and Leydig cells. In the prostate, ER was observed in epithelial cells of tubulo-alveoli, while the stroma was unlabelled. ER was not found to be expressed in any prostate cells. In the prostate, AR was detected in nuclei of epithelial, stromal and endothelial cells. In seminal vesicles, staining of ER was found in nuclei of epithelial and stromal cells. Similar findings were observed using AR antibodies. While ER mRNA could not be detected by in situ hybridization, weak staining for ER was localized in epithelial cells of seminal vesicles. In the ovary, both ER and ER were found to be expressed. ER mRNA was found in granulosa cells of growing follicles, while ER was present in theca cells, interstitial gland cells and germinal epithelium. AR immunoreactivity was detected in granulosa cell nuclei in growing follicles and also in scattered interstitial cells. In the oviduct and uterus, ER was observed in nuclei of epithelial cells as well as of stromal and muscle cells. Similarly, AR immunoreactivity was present in nuclei of epithelial cells, stromal and muscle cells in both the oviduct and uterus. ER was not detected in the oviduct and uterus. The present findings indicate a cell-specific localization of ER , ER and AR in reproductive tissues in rats of both sexes. By establishing the precise sites of action of oestrogens and androgens they contribute to a better understanding of the respective role of these steroids in reproduction function.

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“…Both receptors are expressed in adult and immature ovary, the ER␣ protein being detected in interstitial and theca cells and the ER␤ protein in granulosa cells (Fitzpatrick et al, 1999;Hiroi et al, 1999;O'Brien et al, 1999;Sar and Welsch, 1999). Analysis of single and compound mutant mice for ER␣ and ER␤ has provided evidence that each receptor is required for specific ovarian functions, namely follicular development (ER␤) and ovulation (ER␣) (Dupont et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Expression of Sox9 in granulosa cells lacking the estrogen receptors, ERα and ERβ

Dupont

Dennefeld

Krust

et al. 2002

Developmental Dynamics

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Differential immunolocalization of estrogen receptor alpha and beta in rat ovary and uterus

Cited by 135 publications

References 44 publications

Rapid and estrogen receptor beta mediated actions in the hippocampus mediate some functional effects of estrogen

Rapid and estrogen receptor beta mediated actions in the hippocampus mediate some functional effects of estrogen

Localization of oestrogen receptor alpha, oestrogen receptor beta and androgen receptors in the rat reproductive organs

Expression of Sox9 in granulosa cells lacking the estrogen receptors, ERα and ERβ

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