Differential in vitro phenotype pattern, transforming growth factor-β 1 activity and mRNA expression of transforming growth factor-β 1 in Apert osteoblasts

Locci, Paola; Baroni, Tiziano; Pezzetti, Furio; Lilli, Cinzia; Marinucci, Lorella; Martinese, Domenica; Becchetti, Ennio; Calvitti, Mario

doi:10.1007/s004410051374

Cited by 23 publications

(13 citation statements)

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“…Quantitative and qualitative alterations of GAG may be induced by modifying the extracellular environment that alters connective tissue cell metabolism. [38][39][40][41][42][43][44] Therefore, any variation in GAG composition can influence the tissue's state of health. Both the whole appliance and the single components of the appliance reduced the cellular and extracellular accumulation of GAG.…”

Section: Discussionmentioning

confidence: 99%

In vitro cytotoxic effects of orthodontic appliances

Locci

Lilli

Marinucci

et al. 2000

J. Biomed. Mater. Res.

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The objective of this study was to evaluate the effects of an orthodontic appliance and of its components (brackets, bands, and arch wires) on some cell functions. Fibroblasts were cultured either in the presence of one unwashed orthodontic appliance, or one orthodontic appliance immersed in MEM for 28 days before use (washed appliance), or in the presence of MEM in which the appliances had been immersed. At the end of in vitro maintenance, morphological studies were carried out with SEM and TEM. Cell proliferation and GAG synthesis and secretion by radio-labeled precursors were assessed. The data indicated that unwashed appliances were more cytotoxic than washed ones. Moreover, the arch wire was the most biocompatible component of the orthodontic appliance, and the bracket was the least biocompatible. A comparative study into the effects on cell proliferation of the most common metal ions released by the appliances was also carried out. At the concentration released by one orthodontic appliance immersed for 28 days, the highest reduction in DNA synthesis was observed in the presence of Cu(++).

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Section: Discussionmentioning

confidence: 99%

In vitro cytotoxic effects of orthodontic appliances

Locci

Lilli

Marinucci

et al. 2000

J. Biomed. Mater. Res.

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“…One factor may be the imbalance between growth factor beta 1 (TGF A1) and basic fibroblast growth factor (FGF-b) in the extracellular matrix of Apert calvaria. 42 Another possible explanation is the lower interleukins (IL1 and IL6) noted in Apert periosteal fibroblasts. 43 The extracellular matrix of the calvaria of Crouzon patients also show increased type I collagen (similar to Apert syndrome).…”

Section: Fibronectin and Neural Crest Cellsmentioning

confidence: 99%

Fibronectin and Craniofacial Surgery

Al‐Qattan

Alshomer²,

Alqahtani³

et al. 2014

Annals of Plastic Surgery

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Fibronectin is an essential component of the extracellular matrix. The role of fibronectin in craniofacial surgery has not been previously reviewed. Fibronectin mediates bone differentiation and development of the skull. Studies have shown that normal development of the skull requires a specific pattern of expression around the epithelial-mesenchymal interface of the neurocranium. Fibronectin is also essential in mediating the migration of neural crest cells to form the facial skeleton. The calvaria of patients with Apert and Crouzon syndromes have an abnormally elevated collagen level. However, fibronectin levels are elevated in the former syndrome and decreased in the latter syndrome. The significance of this requires further research. Fibronectin gene expression is increased in port wine-derived fibroblasts in patients with Sturge-Weber syndrome. Normal palatogenesis also requires a specific pattern of expression of fibronectin around the maxillary process as well as the roof of the stomodeum, and several studies have linked the development of cleft lip/palate to an imbalance of fibronectin content of the extracellular matrix. Fibronectin mediates cell-to-cell attachment during repair of calvarial defects; hence, fibronectin has been used as a carrier for bone morphogenetic proteins to treat calvarial defects. Finally, fibronectin is now an essential component in stem cell technology related to craniofacial surgery.

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“…The observed imbalance in the production of ILs suggests that ILs could be the natural autocrine regulators of ECM production in Apert fibroblasts. Since TGF-β1 is able to modulate ECM macromolecule accumulation in fibroblasts (Bodo et al, 1999b), and a variety of osteoblast activities (Janssens et al, 2005), we also analysed its expression and secretion which resulted both increased in Apert osteoblasts in vitro (Locci et al, 1999). Moreover, the level of TGF-β1 was decreased by the addition of FGF2.…”

Section: Apert Syndromementioning

confidence: 99%

The Fathers of Italian Histology

ISH¹

2009

Eur J Histochem

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Subscriptions, cancellations, business correspondence and any enquiries must be sent to the Tipografia PIME Editrice srl, Pavia, Italy. Cancellations must be received before the end of September to take effect at the end of the same year.No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means (electronic, electrostatic, magnetic type, mechanical, photocopying or otherwise) without written permission by the Publishers.Reg. Tribunale di Pavia n. 289/23.2.1984. Supported by the Ministero per i Beni e le Attività Culturali, Italy as a publication of high cultural value.Associato all'USPI Unione Stampa Periodica Italiana Disclaimer. Whilst every effort is made by the publishers and the editorial board to see that no inaccurate or misleading data, opinion or statement appears in this journal, they wish to make it clear that the data and opinions appearing in the articles or advertisements herein are the responsibility of the contributor or advisor concerned. Accordingly, the publisher, the editorial board and their respective employees, officers and agents accept no liability whatsoever for the consequences of any inaccurate or misleading data, opinion or statement. The Journal publishes original papers, technical reports, letters to the editor, review articles concerning investigations performed with the aid of biophysical, biochemical, molecular-biological, enzymatic, immunohistochemical, cytometric, and image analysis techniques. Areas of particular interest to the European Journal of Histochemistry include: -functional cell and tissue biology in animals and plants; -cell differentiation and death; -cell-cell interaction and molecular trafficking; -biology of cell development and senescence; -nerve and muscle cell biology; -cellular basis of diseases During this period, Carlo Rizzoli established the experimental approach for the study of the molecular basis of cell differentiation in vitro, anticipating some aspects of the present investigation on the potentiality of stem cells. Furthermore, Carlo Rizzoli was one of the first Italian scientists to publish its scientific reports in large-diffusion international journals, thus contributing to the world-wide diffusion of the seminal studies on the in vitro cell differentiation models. Editor-in-ChiefIn 1961, Carlo Rizzoli became Professor of Histology and general embryology and, since 1964 to 1999, Director of the Institute of Histology at the University of Bologna.The initial steps of this undertaking were challenging, since in 1963, following the recruitment of Oliviero Mario Olivo at the Chair of Human Anatomy, the facilities of the Institute of Histology were almost nonexistent. In few years, however, Carlo Rizzoli was able to organize an efficient research group of motivated young collaborators that included Paolo Carinci, Lia Guidotti, Francesco Antonio Manzoli, capable of introducing original and seminal lines of research into the national and international histological arena. In this way, a numb...

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Differential in vitro phenotype pattern, transforming growth factor-β 1 activity and mRNA expression of transforming growth factor-β 1 in Apert osteoblasts

Cited by 23 publications

References 46 publications

In vitro cytotoxic effects of orthodontic appliances

In vitro cytotoxic effects of orthodontic appliances

Fibronectin and Craniofacial Surgery

The Fathers of Italian Histology

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