Differential induction of phenylalanine ammonia-lyase gene expression in response to in vitro callus unions of Prunus spp.

Pina, Ana; Errea, P.

doi:10.1016/j.jplph.2007.05.015

Cited by 98 publications

(68 citation statements)

References 25 publications

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“…Plantas com esse tipo de incompatibilidade apresentam desenvolvimento lento, proporcional ao grau de descontinuidade da união (ERMEL et al, 1999;HARTMANN, et al, 2010). Os sintomas externos são pouco evidentes, podendo tardar vários anos para que sejam identifi cados (PINA & ERREA, 2008). Em estágios avançados, há esgotamento do sistema radicular devido à translocação defi ciente no ponto de união (ZARROUK et al, 2010).…”

Section: Incompatibilidade 'Localizada'unclassified

“…Inúmeros autores têm relacionado à incompatibilidade de enxertia com diferenças quantitativas e/ou qualitativas no conteúdo de fenóis, além de mudanças na atividade das enzimas que regulam a síntese desses compostos (ERREA, 1998;RODRIGUES et al, 2002;MNG'OMBA et al, 2008;PINA & ERREA, 2008;TELLES et al, 2009;ZARROUK et al, 2010;YUAN et al, 2011;PEREIRA et al, 2013). Segundo ERREA (1998), os compostos fenólicos estão implicados em todos os eventos envolvidos no processo de enxertia.…”

Section: Compostos Fenólicosunclassified

“…Estudos recentes de expressão gênica apontam para o envolvimento de ezimas chave da vía fenilpropanoide, como a fenilalanina amônia-liase (PAL), a cianamato 4-hidroxilase (C4H) e a 4-cumarato Coa-ligase (4CL), no mecanismo de incompatibilidade entre espécies de Prunus (PINA & ERREA, 2008, PEREIRA, 2012PEREIRA et al, 2013). Os resultados desses estudos indicam que, além de alterações anatômicas, fi siológicas e bioquímicas, mudanças moleculares também estão envolvidas no fenômeno.…”

Section: Compostos Fenólicosunclassified

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Incompatibilidade de enxertia em Prunus

et al. 2014

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Section: Incompatibilidade 'Localizada'unclassified

Section: Compostos Fenólicosunclassified

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Incompatibilidade de enxertia em Prunus

et al. 2014

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“…Staining for the presence of flavonoids was performed according to (Pina and Errea 2008). Briefly, decolorized leaf disks were stained for 10 min in 1% (w/v) Naturstoff reagent A (2-Aminoethyl diphenylborinate, Fluka) in ethanol and then washed 4 times with ethanol.…”

Section: Flavonoid Stainingmentioning

confidence: 99%

Identification of genes expressed during the compatible interaction of grapevine with Plasmopara viticola through suppression subtractive hybridization (SSH)

Legay¹,

Marouf²,

Berger³

et al. 2010

The Downy Mildews - Biology, Mechanisms of Resistance and Population Ecology

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Grapevine (Vitis vinifera) is the most widely cultivated and economically important fruit crop, but is susceptible to a large number of diseases. Downy mildew, caused by the obligate biotrophic oomycete pathogen Plasmopara viticola, is a common disease present in all regions where vines are cultivated. We used suppression subtractive hybridization (SSH) to generate two cDNA libraries enriched for transcripts induced and repressed, respectively, in the susceptible grapevine cultivar Chasselas 24 h after inoculation with P. viticola. Differential screening on glass slide microarrays yielded over 800 putative genes that were upregulated in response to P. viticola infection and over 200 that were down-regulated. One hundred and ninety four of these, were sequenced, identified and functionally categorised. Transcript abundance of twelve genes over a 48 h time course was examined by reverse transcriptase quantitative real-time PCR (RT-qPCR). Ten of these genes were induced/enhanced by P. viticola challenge, confirming the results of the SSH. The vast majority of the genes identified are related to defence. Interestingly, many genes involved in photosynthesis were down-regulated.

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“…As precursor, it may lead to the aromatic amino acids synthesis, by setting free the amine group (NH 3 ) to form phenylalanine and tryptophan. Phenylalanine acts as an intermediate in the biosynthesis of phenolic compounds such as flavones, isoflavones and flavonoids (Pina and Errea, 2008;Tzin and Galili, 2010). In this paper, it is possible that part of the absorbed glutamine has been deviated for the secondary metabolites biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Proteic and phenolics compounds contents in Bacupari callus cultured with glutamine and nitrogen sources

et al. 2017

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In this study was evaluated the influence of glutamine supplementation on the endogenous content of amino acids, proteins, total phenolics, flavonoids and proanthocyanidins in Bacupari callus. The explants were inoculated in MS medium, MS with half concentration of the nitrogen salts (MS½) and nitrogen-free MS, supplemented with glutamine (5, 10, 30 and 60mM) named as Gln5, Gln10, Gln30 and Gln60. Amino acids and proteins were analyzed after 20, 80 and 140 days and the secondary metabolites on the 140 th day. There was no difference in the amino acids on the 20 th day. On the 80 th day the treatments MS and MS½ presented the lowest levels. On the 140 th day MS and MS½ presented the lowest amino acid concentration and Gln10 the highest. Concerning proteins, there was difference only on the 140 th day, being the highest concentrations observed in Gln5, and the lowest in MS½ treatment. Total phenolics content was higher in the treatment Gln60 and lowest in MS. Treatments Gln5, Gln10, Gln30 and MS½ were statistically equal. For flavonoids, the highest values occurred in the treatments Gln30, Gln60 and MS½ and the lowest in Gln5, Gln10 and MS. Similarly, for the proanthocyanidins the highest concentrations were observed in treatment Gln60 and the lowest in Gln5 and MS. In conclusion, the treatment with 60mM of glutamine favors the protein accumulation and production of secondary metabolites in Bacupari callus.Keywords: glutamine, phenols, flavonoids, proanthocyanidins, amino acids. Conteúdo de proteínas e compostos fenólicos em calos de Bacupari cultivados com glutamina e fontes de nitrogênio ResumoNesse estudo foi avaliado o efeito da suplementação com glutamina no conteúdo endógeno de aminoácidos, proteínas, fenólicos totais, flavonoides e proantocianidinas em calos de Bacupari. Os explantes foram inoculados em meio MS, meio MS com metade da concentração de dos sais de nitrogênio (MS½) e meio MS sem nitrogênio suplementado com glutamina (5, 10, 30 e 60mM) denominados como Gln5, Gln10, Gln30 e Gln60. Os aminoácidos e as proteínas foram analisados após 20, 80 e 140 dias e os metabólitos secundários no 140° dia. Não houve diferença nos aminoácidos no 20° dia. No 80° dia os tratamentos MS e MS½ apresentaram os menores níveis. No 140° dia, MS e MS½ apresentaram as menores concentrações de aminoácidos e o Gln10 as maiores. A respeito das proteínas, houve diferença apenas no 140° dia, sendo as maiores concentrações observadas nos tratamentos Gln, e as menores no MS½. O conteúdo de fenólicos totais foi maior no tratamento Gln60 e menor no MS. Os tratamentos Gln5, Gln10, Gln30 e MS½ foram estatisticamente iguais. Para os flavonóides, os maiores valores ocorreram nos tratamentos Gln30, Gln60 e MS½ e os menores no Gln5, Gln10 e MS. Da mesma forma, para as proantocianidinas, as maiores concentrações foram observadas no tratamento Gln60 os menores no Gln5 e MS. Em conclusão, o tratamento com 60 mM de glutamina favorece o acúmulo de proteínas e a produção de metabólitos secundários em calos de Bacupari.

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Differential induction of phenylalanine ammonia-lyase gene expression in response to in vitro callus unions of Prunus spp.

Cited by 98 publications

References 25 publications

Incompatibilidade de enxertia em Prunus

Incompatibilidade de enxertia em Prunus

Identification of genes expressed during the compatible interaction of grapevine with Plasmopara viticola through suppression subtractive hybridization (SSH)

Proteic and phenolics compounds contents in Bacupari callus cultured with glutamine and nitrogen sources

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