Differential inflammatory response of dental pulp explants and fibroblasts to saliva

Cvikl, Barbara; Lussi, Adrian; Moritz, Andreas; Sawada, Kosaku; Gruber, Reinhard

doi:10.1111/iej.12493

Cited by 11 publications

(11 citation statements)

References 20 publications

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“…The inflammatory response requires activation of the intercellular signalling pathways, such as the NF-jb and MAPK pathways (ERK, JNK and, in particular, the p38 MAPK pathway; Botero et al 2010, Cvikl et al 2016. Dentine is permeable due its tubular structure, and after tooth injuries, the diffusion of a variety of molecules, such as bioactive proteins, bacteria and toxins, can occur and initiate inflammatory events.…”

Section: Molecular Events Involved In Inflammatory Responsesmentioning

confidence: 99%

“…Main effects p38 MAPK Pathway (p38 Mitogen-Activated Protein Kinases) Implicated in cell differentiation and proliferation, odontoblast secretory activity and inflammatory response , Cooper et al 2014, Shin et al 2015, Cvikl et al 2016). TGFb-Smad Signalling Pathway (Transforming Growth Factor/Small Mother Against Decapentaplegic Pathway) Implicated in cell differentiation and proliferation (Chang et al 2015) and odontoblast secretory activity (Lucchini et al 2002, He et al 2004).…”

Section: Pathwaymentioning

confidence: 99%

“…Although a low level of inflammation stimulates dentine repair events, exacerbated inflammation impairs dentinogenesis by many mechanisms, including cell death . The inflammatory response requires activation of the intercellular signalling pathways, such as the NF-jb and MAPK pathways (ERK, JNK and, in particular, the p38 MAPK pathway; Botero et al 2010, Cvikl et al 2016. The NF-jb pathway is activated by cytokine receptors, such as TNF-a and interleukins.…”

Section: Molecular Events Involved In Inflammatory Responsesmentioning

confidence: 99%

“…Wnt/b-catenin Signalling Pathway Implicated in odontoblast differentiation and proliferation(Wang et al 2012a, Yoshida et al 2016). NF-kb Pathway (key Nuclear Factor kappa b Pathway) Implicated in inflammatory responses, innate and adaptive immunity and stress responses(Chang et al 2005, Shin et al 2015, Cvikl et al 2016). MAPK/ERK Pathway (Mitogen-Activated Protein Kinase/ Extracellular signal-Regulated Kinase Pathway) Implicated in cell differentiation and proliferation(Bento et al 2013, Wang et al 2016b, odontoblast secretory activity and inflammatory responses(Botero et al 2010, Shin et al 2015, Cvikl et al 2016.…”

mentioning

confidence: 99%

“…NF-kb Pathway (key Nuclear Factor kappa b Pathway) Implicated in inflammatory responses, innate and adaptive immunity and stress responses(Chang et al 2005, Shin et al 2015, Cvikl et al 2016). MAPK/ERK Pathway (Mitogen-Activated Protein Kinase/ Extracellular signal-Regulated Kinase Pathway) Implicated in cell differentiation and proliferation(Bento et al 2013, Wang et al 2016b, odontoblast secretory activity and inflammatory responses(Botero et al 2010, Shin et al 2015, Cvikl et al 2016. JNK Pathway (c-Jun N-terminal Kinase Pathway) Implicated in cell differentiation, odontoblast secretory activity and inflammatory responses(Goda et al 2015, Shin et al 2015.…”

mentioning

confidence: 99%

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Disclosing the physiology of pulp tissue for vital pulp therapy

2018

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The discovery that dentine is a reservoir of bioactive molecules that can be recruited on demand has attracted efforts to develop new protocols and materials for vital pulp therapy (VPT). The noncollagenous proteins (NCPs) present in the dentine extracellular matrix (ECM) include growth factors (TGF-β1, BMP-7, FGF-2, IGF-1 and IGF-2, NGF and GDNF), extracellular matrix molecules (DSP, DPP, BSP, DMP-1 and DSPP) and both anti-inflammatory and pro-inflammatory chemokines and cytokines (TNF-α, IL-1, IL-6 and IL-10). Molecules such as DSP and DPP are mainly expressed by odontoblasts, and they are cleaved products from dentine sialophosphoprotein (DSPP). Some molecules, such as TGF-β1, specifically interact with decorin/biglycan in dentine. Although TGF-β1 increases the expression and secretion of NGF in human pulp cells, NGF induces mineralization and increases the expression of DSPP and DMP-1. Furthermore, GDNF may act as a cell survival factor and mitogen during tooth injury and repair. Pulp capping materials, such as MTA and calcium hydroxide, can solubilize bioactive dentine molecules (TGF-β1, NGF and GDNF) that stimulate tertiary dentinogenesis. The binding of these signalling molecules leads to activation of several signalling transduction pathways involved in dentinogenesis, odontoblast differentiation and inflammatory responses, such as the p38 MAPK, NF-kβ and Wnt/β-catenin signalling pathways. Understanding the cascade of cellular and molecular events underlying the repair and regeneration processes provides a reasonable new approach to VPT through a targeted interaction between tooth tissue and bioactive molecules.

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Section: Molecular Events Involved In Inflammatory Responsesmentioning

confidence: 99%

Section: Pathwaymentioning

confidence: 99%

Section: Molecular Events Involved In Inflammatory Responsesmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Disclosing the physiology of pulp tissue for vital pulp therapy

2018

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Dentin-Pulp Tissue Regeneration Approaches in Dentistry: An Overview and Current Trends

Şişmanoğlu

Ercal

2020

Advances in Experimental Medicine and Biology

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Inhibitory effect of saliva on osteoclastogenesis in vitro requires toll-like receptor 4 signaling

et al. 2017

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ObjectivesSaliva can suppress osteoclastogenesis, but the underlying mechanism has not been discovered yet. Considering that endotoxins suppress osteoclastogenesis in bone marrow cultures and that saliva contains endotoxins, it was reasonable to hypothesize that the impact of saliva on osteoclastogenesis requires toll-like receptor 4 signaling.Material and methodsTo test this hypothesis, we blocked toll-like receptor 4 signaling with TAK-242 in the presence of saliva in murine bone marrow cultures. Osteoclastogenesis was evaluated based on gene expression analysis and histochemical staining for tartrate-resistant acid phosphatase. Resorption was performed on dentine.ResultsWe report that TAK-242 reversed the inhibitory effect of fresh sterile saliva on the formation of multinucleated cells that stained positive for tartrate-resistant acid phosphatase. In line with this finding, TAK-242 increased the expression of the osteoclast functional genes cathepsin K, calcitonin receptor, and tartrate-resistant acid phosphatase in the presence of saliva. TAK-242 also supported the expression of NFATc1, the master regulator of osteoclastogenesis, as well as DC-STAMP and Atp6v0d2, both being cell fusion genes. In support of the hypothesis, depletion of saliva from endotoxin partially reversed the inhibitory effect on osteoclastogenesis. Moreover, salivary pellicle on plastic and titanium did not affect osteoclastogenesis.ConclusionInhibition of toll-like receptor 4 signaling revealed that saliva can contribute to innate immunity by preventing hematopoietic progenitors to become osteoclasts.Clinical relevanceSaliva can activate pattern recognition receptor signaling through endotoxins and other stress factors, indicating the demand for macrophages rather than for osteoclasts.Electronic supplementary materialThe online version of this article (doi:10.1007/s00784-016-2041-7) contains supplementary material, which is available to authorized users.

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Differential inflammatory response of dental pulp explants and fibroblasts to saliva

Abstract: Saliva has a potential impact on inflammation of dental pulp fibroblasts in vitro but not when cells are embedded in the intrinsic extracellular matrix of the explant tissue.

Cited by 11 publications

References 20 publications

Disclosing the physiology of pulp tissue for vital pulp therapy

Disclosing the physiology of pulp tissue for vital pulp therapy

Dentin-Pulp Tissue Regeneration Approaches in Dentistry: An Overview and Current Trends

Inhibitory effect of saliva on osteoclastogenesis in vitro requires toll-like receptor 4 signaling

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