Differential Influence of Pueraria lobata Root Extract and Its Main Isoflavones on Ghrelin Levels in Alcohol-Treated Rats

Abstract

The study was carried out on alcohol-preferring male Wistar rats. The following drugs were repeatedly (28×) administered: acamprosate (500 mg/kg, p.o.), naltrexone (0.1 mg/kg, i.p), and Pueraria lobata (kudzu) root extract (KU) (500 mg/kg, p.o.) and its isoflavones: daidzin (40 mg/kg, p.o.) and puerarin (150 mg/kg, p.o.). Their effects on a voluntary alcohol intake were assessed. KU and alcohol were also given for 9 days in an experiment on alcohol tolerance development. Finally, total and active ghrelin levels in peripheral blood serum were measured by ELISA method. Acamprosate, naltrexone, daidzin, and puerarin, reducing the alcohol intake, caused an increase in both forms of ghrelin levels. On the contrary, though KU inhibited the alcohol intake and alcohol tolerance development, it reduced ghrelin levels in alcohol-preferring rats. The changes of ghrelin concentration could play a role as an indicator of the currently used drugs. The other effect on the KU-induced shift in ghrelin levels in the presence of alcohol requires further detailed study.

Changes in the content of Puerarin-PLGA nanoparticles in mice under the influence of alcohol and analysis of their antialcoholism

“…In addition, puerarin has been shown to have anti-alcohol, liver-protecting and anti-inflammatory effects in previous studies. 29–31 In our experiment, this results were confirmed that PUE-NP had better anti-alcohol, liver-protecting and anti-inflammatory effects on mice with alcoholic liver poisoning. The possible reason is that PLGA nanoparticles delayed the action time of drugs in the target organs, which indirectly enhances the pharmacological effect of Puerarin, Puerarin and PUE-NP also have certain protective effect on the kidney tissue damage.…”

“…These saponins have protective effect on liver tissue immune damage. Puerarin can protect liver injury through gastric absorption and induce apoptosis of activated hepatic stellate cells, 29,30 it could also reverse the chemically-induced liver fibrosis effectively. Studies have shown that when endotoxin from intestinal tract reaches the liver via portal vein, the reticuloendothelial system in liver (mainly Kupffer cells) can recognize and phagocytosis endotoxin through Toll-like receptor 4 (TLR-4) on the cell surface, which was digested and removed by lysosomes.…”

Cannabidiol as a Modulator of the Development of Alcohol Tolerance in Rats

“…The control groups received rapeseed oil (OIL) alone in volumes analogous to the CBD. For the induction of alcohol tolerance, the model used in our previous experiments was applied, with slight modifications [ 4 , 62 , 63 ]. Briefly, 30% EtOH was administered intraperitoneally (i.p.)…”

“…In our previous study, we focused on observing selected aspects of tolerance occurrence based on the assumption that changes in the tolerance development provided by some drugs may be valuable in explaining our observed alteration of the addiction process [ 4 , 5 ].…”

Advances in Extraction, Purification, and Analysis Techniques of the Main Components of Kudzu Root: A Comprehensive Review

“…It covers post-stroke depression as well as depression caused by coexisting conditions, such as diabetes, coronary heart disease, migraines, Parkinson’s disease, and others. KR has the potential for an alcohol intake reduction [ 39 ] and, thus, in the long-term, prevention of alcohol addiction [ 40 ]. Zhou et al gave alcoholic male Wistar rats puerarin, daidzein, and puerarin extract [ 41 ].…”