Differential inhibition of Helicoverpa armigera gut proteinases by proteinase inhibitors of pigeonpea (Cajanus cajan) and its wild relatives

Chougule, Nanasaheb P.; Hivrale, Vandana K.; Chhabda, Pavanjeet J.; Giri, Ashok P.; Kachole, Manvendra S.

doi:10.1016/s0031-9422(03)00375-3

Cited by 40 publications

(44 citation statements)

References 27 publications

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“…A planta possui flor com estrutura característica de autofecundação, contudo a presença de agentes polinizadores (abelhas) aumenta em 97,9% a produção de suas vagens e sementes (Cruz-Garcia & Price, 2011).…”

Section: Ocorrência E Características Botânicas De Cajanus Cajanunclassified

“…E m t o d a p l a n t a h á f i t o q u í m i c o s principalmente alcalóides, saponinas e flavonóides (Das et al, 2013;Nwodo et al, 2011;Chougule et al, 2003;Giri et al, 1998). Na raiz estão presentes a cajaflavanona, a cajaisoflavonona, a 2'-O-metil-cajanona, a cajaquinona, a trihidróxi-isoflavonas, a α e β-amirina, a genisteína e o lupeol.…”

Section: Fitoquímica E Composição Nutricional Do Feijão Guanduunclassified

“…Na raiz estão presentes a cajaflavanona, a cajaisoflavonona, a 2'-O-metil-cajanona, a cajaquinona, a trihidróxi-isoflavonas, a α e β-amirina, a genisteína e o lupeol. Na casca da raiz há isogenisteína-7-Oglicosídeo; nas sementes encontra-se o ácido aspártico, a cajanina e a concajanina; nas folhas o β-sitosterol e o estigmasterol e, em toda planta, há a 2-hidroxigenisteína, a cajanona e a ferreirina, entre outros (Nwodo et al, 2011;Das et al, 2013;Chougule et al, 2003;Giri et al, 1998).…”

Section: Fitoquímica E Composição Nutricional Do Feijão Guanduunclassified

“…O ácido hidroxibenzóico por exemplo, está presente nos extratos C. cajan e pode inibir a formação ou até mesmo reverter a hemácia falcizadas (Ouattara et al, 2004;Rossato et al, 2009;Vieira, 2010). Alguns estudos apontam uma inibição mais eficientes da fenilalanina e de ácido hidroxibenzóico, sugerindo que uma atuação sinérgica entre eles poderia aumentar o efeito inibitório da falcização (Ekeke & Shode, 1988;Iwu et al, 1988;Akojie & Fung, 1992;Chougule et al, 2003;Akinsulie et al, 2005).…”

Section: Feijão Guandu E Doença Falciformeunclassified

“…Outros compostos presentes no guandu, como a cajaminose, tem sido relacionada com melhora da afinidade de Hb S com o oxigênio e com o retardo da falcização, porém foram pouco estudados (Ekeke & Shode, 1988;Iwu et al, 1988;Akojie & Fung, 1992;Chougule et al, 2003;Akinsulie et al, 2005).…”

Section: Feijão Guandu E Doença Falciformeunclassified

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Revisão das Propriedades Medicinais de Cajanus cajanna Doença Falciforme

Cordovil

Cople-Rodrigues

Santos

et al. 2015

Rev. bras. plantas med.

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RESUMO:Este artigo descreve a ocorrência, características botânicas, fitoquímicas e a composição nutricional do feijão guandu [Cajanus cajan (L.) Millsp], e sua relação no processo de inibição da falcização na doença falciforme, um distúrbio genético que acomete as hemácias, gerando hemólise e anemia crônica. Dois constituintes químicos estariam mais relacionados aos efeitos inibitórios na falcização de células falciformes: L-fenilalanina e o ácido p-hidroxibenzóico. Estudos químico-biológicos detalhados com o feijão guandu no Brasil poderão esclarecer melhor os mecanismos pelos quais ocorre a inibição da falcização das hemácias e a diminuição do estresse oxidativo, ajudando no tratamento de pessoas com DF.Palavras-chave: propriedades medicinais, cajanus cajan, anemia falciforme. ABSTRACT: Review of Medicinal Cajanus cajan of properties in Sickle Cell Disease.This article describes the occurrence, botanical characteristics, phytochemical and nutritional composition of pigeonpea [Cajanus cajan (L.) Millsp], and their relationship in the process inhibition of sickling in sickle cell disease (SCD), a genetic disorder that affects red blood cells, causing hemolysis and chronic anemia. Two chemical components would be related to the inhibitory effect on sickling of sickle cells: the L-phenylalanine and the p-hydroxybenzoic acid. In Brazil, detailed studies with pigeonpea chemical-biological may clarify the mechanisms by which the inhibition of sickling of red blood cells occurs, reducing oxidative stress and thus helping treating people affected by this disease.

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Section: Ocorrência E Características Botânicas De Cajanus Cajanunclassified

Section: Fitoquímica E Composição Nutricional Do Feijão Guanduunclassified

Section: Feijão Guandu E Doença Falciformeunclassified

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Revisão das Propriedades Medicinais de Cajanus cajanna Doença Falciforme

Cordovil

Cople-Rodrigues

Santos

et al. 2015

Rev. bras. plantas med.

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KINETIC ASSESSMENT AND EFFECT ON DEVELOPMENTAL PHYSIOLOGY OF A TRYPSIN INHIBITOR FROM Eugenia jambolana (JAMBUL) SEEDS ON Helicoverpa armigera (HÜBNER)

Singh

Jamal

Pandey

2014

Arch Insect Biochem Physiol

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A trypsin inhibitor was purified from the seeds of Eugenia jambolana (Jambul) with a fold purification of 14.28 and a yield recovery of 2.8%. Electrophoretic analysis of E. jambolana trypsin inhibitor (EjTI) revealed a molecular weight of approximately 17.4 kDa on 12% denaturing polyacrylamide gel electrophoresis with or without reduction. EjTI exhibited high stability over a wide range of temperatures (4–80 °C for 30 min) and pH (3.0–10.0) and inhibited trypsin‐like activities of the midgut proteinases of fourth instar Helicoverpa armigera larvae by approximately 86%. Feeding assays containing 0.05, 0.15, and 0.45 (% w/w) EjTI on functionally important fourth‐instar larvae indicated a dose‐dependent downfall in the larval body weight as well as on extent of survival. The nutritional analysis suggests that EjTI exerts toxic effects on H. armigera. Dixon plot analysis revealed competitive inhibition of larval midgut proteinases by EjTI, with an inhibition constant (Ki) of approximately 3.1 × 10−9 M. However, inhibitor kinetics using double reciprocal plots for trypsin inhibition demonstrated a mixed inhibition pattern. These observations suggest the potential of E. jambolana trypsin inhibitor protein in insect pest management.

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Characterization and in planta validation of a CHI4 chitinase from Cajanus platycarpus (Benth.) Maesen for its efficacy against pod borer, Helicoverpa armigera (Hübner)

Rathinam

Marimuthu

Tyagi

et al. 2021

Pest Management Science

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BACKGROUND Pigeonpea, Cajanus cajan is one of the economically important legume food crops and a major source of dietary proteins. Management of pod borer, Helicoverpa armigera has been prominent among crop improvement programs. Lack of resistance sources in the cultivated germplasm and crossing incompatibility with pod borer‐resistant wild relatives have prompted biotechnological interventions. Identification and exploitation of genes from pigeonpea wild relatives in host plant resistance towards the pod borer assumes pertinence. Dynamic transcriptome analysis of the wild relative vis a vis cultivated pigeonpea identified a CHI4 chitinase as one of the putative insect resistance genes. RESULTS The study presents variations in important amino acids in CHI4 chitinases from C. cajan and its wild relative C. platycarpus. Comparative protein modeling and docking analysis of the two proteins demonstrated differences in substrate binding efficacy of the chitinase from C. platycarpus which resulted in a minimum binding energy of −8.7 kcal mol−1. Furthermore, we successfully evaluated the insecticidal activity of the chitinase from C. platycarpus against H. armigera challenge through heterologous expression in tobacco. Molecular characterization of transgenic plants confirmed that their efficacy against H. armigera was a result of the integration of CHI4 from C. platycarpus. CONCLUSION Docking analysis demonstrated effective substrate interaction as a possible reason for efficacy against pod borer in the chitinase from C. platycarpus. This was authenticated by successful overexpression and bioefficacy assessment against H. armigera in tobacco. The CHI4 gene from C. platycarpus can be useful in the mitigation of H. armegira in pigeonpea as well as in other crops. © 2021 Society of Chemical Industry

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Differential inhibition of Helicoverpa armigera gut proteinases by proteinase inhibitors of pigeonpea (Cajanus cajan) and its wild relatives

Cited by 40 publications

References 27 publications

Revisão das Propriedades Medicinais de Cajanus cajanna Doença Falciforme

Revisão das Propriedades Medicinais de Cajanus cajanna Doença Falciforme

KINETIC ASSESSMENT AND EFFECT ON DEVELOPMENTAL PHYSIOLOGY OF A TRYPSIN INHIBITOR FROM Eugenia jambolana (JAMBUL) SEEDS ON Helicoverpa armigera (HÜBNER)

Characterization and in planta validation of a CHI4 chitinase from Cajanus platycarpus (Benth.) Maesen for its efficacy against pod borer, Helicoverpa armigera (Hübner)

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