Differential inhibition of progesterone synthesis in bovine luteal cells by estrogens and androgens

Guo, Ing-Cherng; Wu, Leang‐Shin; Lin, Jen H.; Chung, Bon Chu

doi:10.1016/s0024-3205(01)00983-3

Cited by 21 publications

(15 citation statements)

References 31 publications

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“…E 2 at all doses tested was most stimulative on the release of oxytocin during d 5-7 but continuously inhibited oxytocin release from the middle to late luteal phase of the estrous cycle (48). In a recent in vitro study, E inhibited P production by bovine luteal cells in a dose-dependent manner (49). E 2 seems to be less important for the local regulation of bovine luteal function when compared with the pig (50).…”

Section: Discussionmentioning

confidence: 76%

Expression of Estrogen and Progesterone Receptors in the Bovine Ovary During Estrous Cycle and Pregnancy

Berisha¹,

Pfaffl²,

Schams³

2002

ENDO

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Section: Discussionmentioning

confidence: 76%

Expression of Estrogen and Progesterone Receptors in the Bovine Ovary During Estrous Cycle and Pregnancy

Berisha¹,

Pfaffl²,

Schams³

2002

ENDO

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“…Numerous studies have shown that progestins oppose the biological effects of estrogens in many systems (Guo et al, 2001;Zhu et al, 2000). Such functional interplay between estrogen and progesterone is fundamental to maintain significant physiological process (Rose, 1996).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of progesterone receptor activity in recombinant yeast by soot from fossil fuel combustion emissions and air particulate materials

Wang

Xie

Kettrup

et al. 2005

Science of The Total Environment

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“…The protocol for western blotting was modified from the previous publication (Guo et al 2001). Briefly, cell lysates were extracted with the lysis buffer (10 mM Tris-HCl (pH 7.5), 150 mM NaCl, 10% glycerol, 1% Triton X-100, 1 mM dithiothreitol, 0.2 mM phenylmethylsulphonyl fluoride, 1 mg/ml aproptinin, 1 mg/ml leupeptin, 1 mM Na 3 VO 4 , and 1 mM NaF) and separated on 12% SDS-PAGE gel.…”

Section: Western Blotting Analysismentioning

confidence: 99%

Leptin induces proliferation and anti-apoptosis in human hepatocarcinoma cells by up-regulating cyclin D1 and down-regulating Bax via a Janus kinase 2-linked pathway

Chen¹,

Chang²,

Liu³

et al. 2007

Endocr Relat Cancer

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Obesity serves as an important risk factor for incidences of both cirrhotic and non-cirrhotic hepatocellular carcinoma (HCC), which is the third leading cause of cancer death worldwide. Leptin, the obesity biomarker molecule secreted systemically by body fat mass and locally by activated hepatic stellate cells, is proposed to play a certain role in HCC growth. Here, we show both proliferative and anti-apoptotic effects of leptin in HCC cells. Leptin stimulated cyclin D1 promoter activity to increase cyclin D1 protein expression, which accelerated the cell cycle progression. The reduced ratio between anti-apoptotic (Bcl-2) and pro-apoptotic (Bax) Bcl-2 family proteins by transforming growth factor (TGF)-b1 caused HCC cells degradation of poly(ADPribose) polymerase and consequential apoptosis; whereas, leptin protected cells from apoptosis by reversing TGF-b1-reduced Bcl-2/Bax ratio as a result of down-regulating Bax. Any inhibitor specific for Janus kinase 2 (JAK2), phosphatidylinositol 3-kinase (PI3K)/Akt, or mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase 1/2 (ERK1/2) blocked these leptin functions. When intrahepatocytic JAK2 was activated by leptin, the active JAK2 afterward triggered a signaling cascade involving activations of PI3K/Akt and MEK/ERK1/2 in order of occurrence. As yet, in most cases, the crosstalks among signaling pathways primarily studied in diverse cancer cell types for mediating somatotropic effect of leptin are not well clarified and seem to be cell-type dependent. For the first time, our results demonstrate the direct effects of leptin on HCC growth and define its signal pathway with a crosstalking JAK2-PI3K/Akt-MEK/ERK1/2 connection. The identified hierarchy of intrahepatocytic leptin signaling pathway provides a clear basis potentially beneficial to make accurate and effectual strategies for facing both cirrhotic and non-cirrhotic liver carcinogenesis.

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Differential inhibition of progesterone synthesis in bovine luteal cells by estrogens and androgens

Cited by 21 publications

References 31 publications

Expression of Estrogen and Progesterone Receptors in the Bovine Ovary During Estrous Cycle and Pregnancy

Expression of Estrogen and Progesterone Receptors in the Bovine Ovary During Estrous Cycle and Pregnancy

Inhibition of progesterone receptor activity in recombinant yeast by soot from fossil fuel combustion emissions and air particulate materials

Leptin induces proliferation and anti-apoptosis in human hepatocarcinoma cells by up-regulating cyclin D1 and down-regulating Bax via a Janus kinase 2-linked pathway

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