Differential Inhibition of Telomerase Activity during Induction of Differentiation in Hematopoietic, Melanoma, and Glioma Cells in Culture

Cheng, Ann‐Joy; Liao, Shuen Kuel; Chow, Shu Er; Chen, Jan Kan; Wang, Tzu-Chien V.

doi:10.1006/bbrc.1997.7157

Cited by 18 publications

(20 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These data will therefore be of interest in examining the basis for the detection of telomerase activity in normal and malignant haematopoietic cells (Bodnar et al, 1996;Cheng et al, 1997;Holt et al, 1997;Norrback and Roos, 1997;Pan et al, 1997). The human and mouse telomerase RNA genes do share an interesting similarity, in that they both lie in CpG islands, and thus their expression may be regulated by methylation.…”

Section: Discussionmentioning

confidence: 99%

Cloning and characterization of human and mouse telomerase RNA gene promoter sequences

et al. 1998

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Cloning and characterization of human and mouse telomerase RNA gene promoter sequences

et al. 1998

View full text Add to dashboard Cite

“…Inhibition of telomerase and repression of hTERT expression have been observed in a variety of cell lines upon induction of cellular differentiation by several reagents, such as retinoic acid and dimethyl sulfoxide (31,109,130,208,258,270). The repression of telomerase is clearly not required for cell differentiation, since cells overexpressing an exogenous telomerase differentiate normally (230).…”

Section: Transcriptional Regulation Of Htert Genementioning

confidence: 99%

Human Telomerase and Its Regulation

Cong

Wright

2002

Microbiol Mol Biol Rev

760

668

View full text Add to dashboard Cite

The telomere is a special functional complex at the end of linear eukaryotic chromosomes, consisting of tandem repeat DNA sequences and associated proteins. It is essential for maintaining the integrity and stability of linear eukaryotic genomes. Telomere length regulation and maintenance contribute to normal human cellular aging and human diseases. The synthesis of telomeres is mainly achieved by the cellular reverse transcriptase telomerase, an RNA-dependent DNA polymerase that adds telomeric DNA to telomeres. Expression of telomerase is usually required for cell immortalization and long-term tumor growth. In humans, telomerase activity is tightly regulated during development and oncogenesis. The modulation of telomerase activity may therefore have important implications in antiaging and anticancer therapy. This review describes the currently known components of the telomerase complex and attempts to provide an update on the molecular mechanisms of human telomerase regulation

show abstract

“…[2][3][4][5] So, research to find new drugs that can induce differentiation and apoptosis of cancer cells by telomerase manipulation has become a central focus. For example, all-trans retinoic acid has recently been identified to induce apoptosis and differentiation in pro-myelocytic leukemia cells by telomerase suppression.…”

Section: Discussionmentioning

confidence: 99%

“…There has been some studies reporting a direct link between decreased telomerase activity and terminal differentiation in leukemic cells and normal hematopoietic stem cells. 4,5) However, it is still not determined whether the decreased telomerase activity causes differentiation of stem cells or differentiated stem cells have decreased telomerase activity. To answer this question, the effects of telomerase inhibition on hematopoietic stem cells was investigated in the only one previous study.…”

Section: Introductionmentioning

confidence: 99%

Adenovirus-Mediated Antisense Vector-Induced Inhibition of Human Telomerase RNA May Induce Differentiation of CD34+ Cells

Kim

Song

et al. 2006

Korean J Hematol

View full text Add to dashboard Cite

Background: Background Telomerase activation and human telomerase RNA (hTR) expression are known to be related to the preservation of the "stemness" of stem cells. In this study, we have inhibited the expression of hTR to find the relationship between the telomerase activity and differentiation of normal hematopoietic stem cells. Methods: We used cord blood collected from 10 full term pregnant women. We classified the CD34+ hematopoietic stem cells from the same donor into three groups: the Ad-OA group was treated with the recombinant adenoviral (Ad) vector Ad-OA using telomerase antisense, the Ad-M6 group was treated with a mutant version of the Ad-OA without telomerase antisense, and a control group without any treatment. Results:The mean number of colony-forming cells (CFCs) were 110±38 for the Ad-OA groups, 540±56 for the Ad-M6 groups, and 650±72 for the control groups. Thus, CFCs in the Ad-OA group were lower than in the Ad-M6 group (P＜0.01). The myeloid portion of the CFCs in the Ad-OA group was higher than the Ad-M6 and control groups (P＜0.01). The Ad-OA group showed a higher percentage of granulocytes suggesting more of a tendency for myeloid differentiation than the Ad-M6 and control groups (P＜0.01). We found that the suppression of telomerase activity by the antisense telomerase adenovirus induced the differentiation of hematopoietic stem cells confirmed by differential cell count and cytochemical staining.

show abstract

Differential Inhibition of Telomerase Activity during Induction of Differentiation in Hematopoietic, Melanoma, and Glioma Cells in Culture

Cited by 18 publications

References 22 publications

Cloning and characterization of human and mouse telomerase RNA gene promoter sequences

Cloning and characterization of human and mouse telomerase RNA gene promoter sequences

Human Telomerase and Its Regulation

Adenovirus-Mediated Antisense Vector-Induced Inhibition of Human Telomerase RNA May Induce Differentiation of CD34+ Cells

Contact Info

Product

Resources

About