Differential inhibitory effects of baicalein and baicalin on LPS-induced cyclooxygenase-2 expression through inhibition of C/EBPβ DNA-binding activity

Woo, Kyung Jin; Lim, Jun Hee; Suh, Seong-Il; Kwon, Young-Kyu; Shin, Sang-Woo; Kim, Sang Chan; Choi, Yung Hyun; Park, Jong‐Wook; Kwon, Taeg Kyu

doi:10.1016/j.imbio.2006.02.002

Cited by 113 publications

(61 citation statements)

References 32 publications

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“…TJ-9 contains several flavonoids such as baicalein, baicalin and wogonin. 17) In particular, baicalein (but not baicalin) 18) and wogonin 19) are reported to suppress COX-2 expression in LPS-treated mouse macrophage. Moreover, wogonin suppresses COX-2 expression in mouse skin fibroblasts treated with tetradeanoyl phorbol acetate (TPA), IL-1b or TNF-a.…”

Section: Discussionmentioning

confidence: 99%

“…IL-6 and IL-8 expression may be due to the difference in transcriptional regulation in promoter region. Baicalein inhibits CCAAT/enhancer binding protein b (C/EBPb) DNAbinding activity in COX-2 promoter region and suppresses COX-2 expression in mouse macrophages, 18) while C/EBPb plays no or little role in IL-6 and IL-8 transcription in LPStreated dermal fibroblasts and LPS-treated rheumatoid arthritis synovial fibroblasts. 22) Taken together, our results suggest that the mechanism of anti-inflammatory effects of TJ-9 is mainly the suppression of PGE 2 production rather than those of inflammatory cytokines.…”

Section: Discussionmentioning

confidence: 99%

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Preventive Effects of a Kampo Medicine, Shosaikoto, on Inflammatory Responses in LPS-Treated Human Gingival Fibroblasts

Ara

Maeda

Fujinami

et al. 2008

Biological & Pharmaceutical Bulletin

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In the present study, we investigated the anti-inflammatory effects of a Kampo medicine Shosaikoto (TJ-9) using in vitro periodontal disease model, in which human gingival fibroblasts (HGFs) treated with lipopolysaccharide (LPS) from Porphyromonas gingivalis (PgLPS) produce IL-6, IL-8 and prostaglandin E 2 (PGE 2 ). Treatment with PgLPS (10 ng/ml), TJ-9 (up to 1 mg/ml) and their combinations for 24 h did not affect the viability of HGFs. Moreover, TJ-9 did not alter LPS-induced IL-6 and IL-8 productions. However, TJ-9 significantly suppressed LPS-induced PGE 2 production in a dose-dependent manner but TJ-9 alone did not affect basal PGE 2 level. Western blotting demonstrated that TJ-9 decreased cyclooxygenase-2 (COX-2) expression in a dosedependent manner but not phospholipase A 2 . Moreover, TJ-9 selectively and dose-dependently inhibited COX-2 activity. These results suggest that TJ-9 decreased PGE 2 production by inhibition of both COX-2 expression and activity and that TJ-9 may be useful to improve gingival inflammation in periodontal disease.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Preventive Effects of a Kampo Medicine, Shosaikoto, on Inflammatory Responses in LPS-Treated Human Gingival Fibroblasts

Ara

Maeda

Fujinami

et al. 2008

Biological & Pharmaceutical Bulletin

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“…Thus far, studies have indicated that flavanols [72,105], flavones [34,87,99,151,186], and flavonols [35] are all capable of inhibiting the release of NO…”

Section: Extracellular Signal-regulated Protein Kinase Pathwaymentioning

confidence: 99%

The interactions of flavonoids within neuronal signalling pathways

2007

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Emerging evidence suggests that dietary phytochemicals, in particular flavonoids, may exert beneficial effects in the central nervous system by protecting neurons against stress-induced injury, by suppressing neuroinflammation and by promoting neurocognitive performance, through changes in synaptic plasticity. It is likely that flavonoids exert such effects in neurons, through selective actions on different components within a number of protein kinase and lipid kinase signalling cascades, such as phosphatidylinositol-3 kinase (PI3K)/Akt, protein kinase C and mitogen-activated protein kinase. This review details the potential inhibitory or stimulatory actions of flavonoids within these pathways, and describes how such interactions are likely to affect cellular function through changes in the activation state of target molecules and/or by modulating gene expression. Although, precise sites of action are presently unknown, their abilities to: (1) bind to ATP binding sites on enzymes and receptors; (2) modulate the activity of kinases directly; (3) affect the function of important phosphatases; (4) preserve neuronal Ca 2+ homeostasis; and (5) modulate signalling cascades lying downstream of kinases, are explored. Future research directions are outlined in relation to their precise site(s) of action within the signalling pathways and the sequence of events that allow them to regulate neuronal function in the central nervous system.

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“…The flavone protects rat brain mitochondria against chronic cerebral hyperfusion-induced oxidative damage (He et al 2009). Its glucoside, baicalin, is a potent anti-inflammatory and antitumor agent (Chang et al 2002;Woo et al 2006;Shang et al 2010). Animal experimentation has shown that baicalin can ameliorate the intestinal cytotoxicity caused by the antitumor agent Cpt-11 (Takasuna et al 1995).…”

Section: Introductionmentioning

confidence: 99%

The effect of cytokinins on shoot proliferation, secondary metabolite production and antioxidant potential in shoot cultures of Scutellaria alpina

Grzegorczyk-Karolak

Kuźma

Wysokińska

2015

Plant Cell Tiss Organ Cult

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The present study evaluates the effects of various cytokinins on Scutellaria alpina shoot proliferation and production of polyphenolic metabolites (baicalin, wogonoside, luteolin, luteolin 7-O-glucoside, verbascoside). The shoots were induced from shoot tips on MS medium supplemented with IAA (indole-3-acetic acid, 0.57 lM) and various concentrations of 6-benzylaminopurine (BAP), kinetin, zeatin (1, 2, 4, 8 lM) or tidiazuron (TDZ) (0.2, 0.5, 1 lM). Among the cytokinins tested, BAP was the most effective for shoot induction, and the highest number of shoots (25 per explant) was achieved with 2 and 4 lM BAP. Maximum biomass production was also achieved on these media. Significantly higher baicalin, wogonoside and verbascoside contents were recorded in treatments containing cytokinins combined with 0.57 lM IAA, when compared to cytokinin-free medium. TDZ at a concentration of 0.5 lM was the most effective for polyphenol production. However, supplementation with cytokinins often results in the reduction of luteolin and its 7-O-glucoside production in the shoot culture of S. alpina. ABTS [2,20 -azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] and ferric reducing antioxidant power assays were used to identify the antioxidant potential of methanolic extracts from shoots cultured in the presence of different types and concentrations of cytokinins. In both tests, the shoots from medium supplemented with 0.5 lM TDZ demonstrated the strongest antioxidant activity. The results indicate that higher polyphenolic content correlated with greater reducing power and antiradical efficiency.

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Differential inhibitory effects of baicalein and baicalin on LPS-induced cyclooxygenase-2 expression through inhibition of C/EBPβ DNA-binding activity

Cited by 113 publications

References 32 publications

Preventive Effects of a Kampo Medicine, Shosaikoto, on Inflammatory Responses in LPS-Treated Human Gingival Fibroblasts

Preventive Effects of a Kampo Medicine, Shosaikoto, on Inflammatory Responses in LPS-Treated Human Gingival Fibroblasts

The interactions of flavonoids within neuronal signalling pathways

The effect of cytokinins on shoot proliferation, secondary metabolite production and antioxidant potential in shoot cultures of Scutellaria alpina

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