Differential internalization of brick shaped iron oxide nanoparticles by endothelial cells

Sun, Zhizhi; Worden, Matthew; Wroczynskyj, Yaroslav; Manna, P. K.; Thliveris, James A.; Hegmann, Torsten; Miller, Donald W.

doi:10.1039/c6tb01480a

Cited by 10 publications

(13 citation statements)

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“…These earlier results on polystyrene nanoshapes are in stark contrast to our own data on IO nanospheres and nanobricks [ 42 ]. We examined the influence of IO NP shape in regulating preferential uptake specifically in endothelial cells (brain, lung).…”

Section: Introductioncontrasting

confidence: 99%

“…While there are numerous papers that describe methods for creating non-spherical IO nanostructures [ 30 ] (particularly wires [ 23 , 31 ], rods [ 32 , 33 , 34 , 35 , 36 ], cubes [ 37 , 38 , 39 ], and sheets [ 40 ]), fewer of those are used or investigated for in-depth (bio)medical research. While there is a wealth of data for T2 MRI contrast enhancement and magnetic hyperthermia use of such IO nanoshapes [ 41 ], specific cell uptake [ 42 ] in various relevant tissue or cancer cell lines as well as their intracellular behavior have remained, to a large extent, unexplored.…”

Section: Introductionmentioning

confidence: 99%

“…Transmission electron microscopy (TEM) revealed differential internalization of nanobricks in endothelial cells compared to epithelial cells. Given the reduced uptake of nanobricks in endothelial cells treated with caveolin inhibitors, the increased expression of caveolin-1 in endothelial cells compared to epithelial cells, and the ability of IO NP nanobricks to interfere with caveolae-mediated endocytosis process, a caveolae-mediated pathway was proposed as the working mechanism for the differential internalization of nanobricks in endothelial cells [ 42 ].…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of Distinct Iron Oxide Nanomaterial Shapes Using Lyotropic Liquid Crystal Solvents

et al. 2017

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A room temperature reduction-hydrolysis of Fe(III) precursors such as FeCl3 or Fe(acac)3 in various lyotropic liquid crystal phases (lamellar, hexagonal columnar, or micellar) formed by a range of ionic or neutral surfactants in H2O is shown to be an effective and mild approach for the preparation of iron oxide (IO) nanomaterials with several morphologies (shapes and dimensions), such as extended thin nanosheets with lateral dimensions of several hundred nanometers as well as smaller nanoflakes and nanodiscs in the tens of nanometers size regime. We will discuss the role of the used surfactants and lyotropic liquid crystal phases as well as the shape and size differences depending upon when and how the resulting nanomaterials were isolated from the reaction mixture. The presented synthetic methodology using lyotropic liquid crystal solvents should be widely applicable to several other transition metal oxides for which the described reduction-hydrolysis reaction sequence is a suitable pathway to obtain nanoscale particles.

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Section: Introductioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Synthesis of Distinct Iron Oxide Nanomaterial Shapes Using Lyotropic Liquid Crystal Solvents

et al. 2017

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“…Alternatively, BSA binding to CNPs promoted their uptake through SR-As due to albumin denaturation upon adsorption [ 173 ]. However, other studies have reported that serum protein adsorption, both in vitro and in vivo, may decrease ION uptake due to attenuation of surface charge and increased nanoparticle agglomeration [ 153 , 174 ]. These adsorbed proteins have also shown to be major contributors to their in vivo recognition by macrophages and the reticuloendothelial system through opsonin receptors.…”

Section: Enhancing Ion Internalizationmentioning

confidence: 99%

Tailoring Iron Oxide Nanoparticles for Efficient Cellular Internalization and Endosomal Escape

et al. 2020

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Iron oxide nanoparticles (IONs) have been widely explored for biomedical applications due to their high biocompatibility, surface-coating versatility, and superparamagnetic properties. Upon exposure to an external magnetic field, IONs can be precisely directed to a region of interest and serve as exceptional delivery vehicles and cellular markers. However, the design of nanocarriers that achieve an efficient endocytic uptake, escape lysosomal degradation, and perform precise intracellular functions is still a challenge for their application in translational medicine. This review highlights several aspects that mediate the activation of the endosomal pathways, as well as the different properties that govern endosomal escape and nuclear transfection of magnetic IONs. In particular, we review a variety of ION surface modification alternatives that have emerged for facilitating their endocytic uptake and their timely escape from endosomes, with special emphasis on how these can be manipulated for the rational design of cell-penetrating vehicles. Moreover, additional modifications for enhancing nuclear transfection are also included in the design of therapeutic vehicles that must overcome this barrier. Understanding these mechanisms opens new perspectives in the strategic development of vehicles for cell tracking, cell imaging and the targeted intracellular delivery of drugs and gene therapy sequences and vectors.

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“…Sun et al investigated the impact of iron oxide nanobricks on mouse‐brain derived microvessel endothelial cells (bEnd.3) in comparison to identically functionalized nanospheres . Both nanobricks and spheres were silanized to attach carboxy groups onto their surfaces, resulting in negatively charged materials (ζ‐potential −39 mV for spheres, −45 mV for bricks).…”

Section: Particle Properties and Resulting Implications On Particle–cmentioning

confidence: 99%

Considerations for the Uptake Characteristic of Inorganic Nanoparticles into Mammalian Cells—Insights Gained by TEM Investigations

2018

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between individual uptake mechanisms for respective nanoparticle systems. Cells internalizing nanoparticles respond to the nanoparticles and different intracellular processes will be initiated, possibly resulting in the impairment of the cellular key functions. [4] Even though these investigations provide data with statistical relevance, they can merely provide an overall picture of the uptake mechanism, as issues of different uptake mechanisms in anisotropic nanoparticle suspensions are barely assessable. Simultaneously, the influence of the biological environment, e.g., the presence of serum proteins, salts, or altered pH conditions, may have a severe impact on the particle properties, which can entail various biological implications. As such, the role of high resolution imaging techniques, which provide a clear evidence for the nanoparticles' fate in the cellular environment, [5] their localization and targeting to different cellular organelles, as well as evidence for uptake and release mechanisms, have gained increasing importance. In particular, transmission electron microscopy (TEM) investigations provide attractive possibilities to study the interaction of particles with biological barriers, i.e., the extracellular membrane or the nuclear membrane, their accumulation into cellular organelles or their spatial distribution on the scale of individual nanoparticles [6] (e.g., uptake as individual nanoparticles or in form of large clusters, etc.). In addition TEM can simultaneously image also the cellular membranes and organelles to determine respective morphological changes [7] upon nanoparticle interaction. [8][9][10][11] Thus, attractive possibilities to acquire additional information on the particle fate inside the cell emerge from TEM investigations.It can be stated here, that the utilization of electron microscopy has been conducted during the last decades [5] and, in view of some extent electron microscopy investigations of particle uptake, can be regarded in this sense as a routinely applied technique. However, frequently TEM is only utilized to characterize the nanoparticle system or to prove particle internalization. Only a fraction of reported studies utilize TEM analysis to reveal details of nanoparticle uptake and internalization or even deduce uptake pathways from these studies. This might be related to the fact that sometimes the interpretation of TEM data is not straightforward but a number of experimental limitations have to be taken into account for such studies. In a recent review, the advantages and disadvantages of TEM investigations in the context of the The internalization of nanoparticles into mammalian cells relies on a complex interplay of several parameters, which enable and dictate uptake and fate of nanoparticles in the cellular system. Due to the complexity of the involved processes, a careful experimental design has to be developed to elucidate peculiarities of the uptake processes of new nanoparticle systems into cells. Individual parameters can be hardly considered alone but h...

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Differential internalization of brick shaped iron oxide nanoparticles by endothelial cells

Abstract: Nonspherical iron oxide core “nanobricks” have enhanced uptake in endothelial cells through caveolae-mediated endocytosis mechanism.

Cited by 10 publications

References 40 publications

Synthesis of Distinct Iron Oxide Nanomaterial Shapes Using Lyotropic Liquid Crystal Solvents

Synthesis of Distinct Iron Oxide Nanomaterial Shapes Using Lyotropic Liquid Crystal Solvents

Tailoring Iron Oxide Nanoparticles for Efficient Cellular Internalization and Endosomal Escape

Considerations for the Uptake Characteristic of Inorganic Nanoparticles into Mammalian Cells—Insights Gained by TEM Investigations

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