Differential involvement of mussel hemocyte sub-populations in the clearance of bacteria

Parisi, Maria Giovanna; Li, Hui; Jouvet, Lionel; Dyrynda, Elisabeth A.; Parrinello, Nicolò; Cammarata, Matteo; Roch, Philippe

doi:10.1016/j.fsi.2008.09.005

Cited by 74 publications

(59 citation statements)

References 51 publications

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“…can have significant influence on the host health, despite the low relative abundance (Vega Thurber et al, 2009). Still, low abundance of the injected strain suggests that other vibrios could Hemolymph microbiota and (a)biotic environment A Lokmer and KM Wegner have contributed to the mortalities, as the exogenous bacteria can be cleared quickly from the hemolymph (Parisi et al, 2008), while stimulating growth of inactive residents (Froelich and Oliver, 2013).…”

Section: Discussionmentioning

confidence: 99%

Hemolymph microbiome of Pacific oysters in response to temperature, temperature stress and infection

2014

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Microbiota provide their hosts with a range of beneficial services, including defense from external pathogens. However, host-associated microbial communities themselves can act as a source of opportunistic pathogens depending on the environment. Marine poikilotherms and their microbiota are strongly influenced by temperature, but experimental studies exploring how temperature affects the interactions between both parties are rare. To assess the effects of temperature, temperature stress and infection on diversity, composition and dynamics of the hemolymph microbiota of Pacific oysters (Crassostrea gigas), we conducted an experiment in a fully-crossed, three-factorial design, in which the temperature acclimated oysters (8 or 22 1C) were exposed to temperature stress and to experimental challenge with a virulent Vibrio sp. strain. We monitored oyster survival and repeatedly collected hemolymph of dead and alive animals to determine the microbiome composition by 16s rRNA gene amplicon pyrosequencing. We found that the microbial dynamics and composition of communities in healthy animals (including infection survivors) were significantly affected by temperature and temperature stress, but not by infection. The response was mediated by changes in the incidence and abundance of operational taxonomic units (OTUs) and accompanied by little change at higher taxonomic levels, indicating dynamic stability of the hemolymph microbiome. Dead and moribund oysters, on the contrary, displayed signs of community structure disruption, characterized by very low diversity and proliferation of few OTUs. We can therefore link short-term responses of host-associated microbial communities to abiotic and biotic factors and assess the potential feedback between microbiota dynamics and host survival during disease.

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Section: Discussionmentioning

confidence: 99%

Hemolymph microbiome of Pacific oysters in response to temperature, temperature stress and infection

2014

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“…Among those pathogens, Vibrio species have been demonstrated to be an important cause of diseases in shellfish [19], which could induce cytotoxicity and the production of reactive oxygen (ROS), etc. [20]. Furthermore, bivalve mollusks are also readily exposed to metallic xenobiotics [21,22].…”

Section: Introductionmentioning

confidence: 99%

The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge

You

Ning²,

Liu³

et al. 2013

Fish & Shellfish Immunology

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a b s t r a c tHeat shock 70 kDa protein 12A (HSPA12A) is an atypical member of HSP70 family, and the translationally controlled tumor protein (TCTP) is a novel HSP with chaperone-like activity. They are both involved in protecting organisms against various stressors. In the present study, the cDNAs of HSPA12A and TCTP (called MgHSPA12A and MgTCTP) were identified from Mytilus galloprovincialis by RACE approaches. The full-length cDNA of MgHSPA12A and MgTCTP encoded a peptide of 491 and 171 amino acids, respectively. Real-time PCR was employed to analyze the tissue distribution and temporal expression of these two genes after bacterial challenge and cadmium (Cd) exposure. It was found that the transcripts of MgHSPA12A and MgTCTP were dominantly expressed in gonad and muscle, respectively. The expression level of MgTCTP at 48 h post Vibrio anguillarum challenge was detected to be significantly up-regulated in hepatopancreas (P < 0.05). As concerned to Cd exposure, 2.0-fold increase of MgHSPA12A expression compared to that of the control was observed at 48 h in 5 mg/L Cd 2þ -treated group, while the expression levels of MgTCTP were significantly decreased after exposed to both 5 and 50 mg/L Cd 2þ for 24 h and 96 h.These results suggested the potential involvement of MgHSPA12A and MgTCTP in the mediation of the immune responses and environmental stress in mussels.

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“…Environmental pollution of coastal and estuarine areas has an impact on immune responses of aquatic organisms Dyrynda et al, 1998;Galloway and Depledge, 2001;Hannam et al, 2009;Pipe and Coles, 1995;Pipe et al, 1999), which is suspected to be associated with an increase of disease susceptibility. Thus, biomarkers that specifically elucidate immunotoxic effects may act as useful tools in integrated chemical and biological effect monitoring programmes.…”

Section: Introductionmentioning

confidence: 99%

“…The haemolymph acts as a carrier of haemocytes, humoral defence factors like soluble lectins, hydrolytic enzymes, antimicrobial peptides, and cellular mediators, such as prophenoloxidase, lectins, cytokines and eicosanoids (Canesi et al, 2006(Canesi et al, , 2002Galloway and Depledge, 2001). Phagocytosis is the predominant mechanism of cellular defence, which, in bivalves, is mainly mediated by circulating and tissue haemocytes (Pipe and Coles, 1995;Pipe et al, 1999). This process proceeds through a number of well-defined stages: recognition, chemotaxis, attachment, respiratory burst, ingestion, and destruction (Galloway and Goven, 2006); and has been focused on as biomarkers for immunotoxic effects in a number of studies (Bussell et al, 2008;Canesi et al, 2007;Pipe et al, 1999;Reid et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Effects of various pollutant mixtures on immune responses of the blue mussel (Mytilus edulis) collected at a salinity gradient in Danish coastal waters

Höher

Köhler

Strand

et al. 2012

Marine Environmental Research

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a b s t r a c tThe Baltic Sea is a semi-enclosed sea with a steady salinity gradient (3&e30&). Organisms have adapted to such low salinities, but are suspected to be more susceptible to stress. Within the frame of the integrated environmental monitoring BONUS þ project "BEAST" the applicability of immune responses of the blue mussel was investigated in Danish coastal waters. The sampling sites were characterised by a salinity range (11e19&) and different mixtures of contaminants (metals, PAHs and POPs), according to chemical analysis of mussel tissues. Variation partitioning (redundancy analysis) was applied to decompose salinity and contamination effects. The results indicated that cellular immune responses (total and differential haemocyte count, phagocytic activity and apoptosis) were mainly influenced by contaminants, whereas humoral factors (haemolytic activity) were mainly impacted by salinity. Hence, cellular immune functions may be suitable as biomarkers in monitoring programmes for the Baltic Sea and other geographic regions with salinity variances of the studied range.Crown

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Differential involvement of mussel hemocyte sub-populations in the clearance of bacteria

Cited by 74 publications

References 51 publications

Hemolymph microbiome of Pacific oysters in response to temperature, temperature stress and infection

Hemolymph microbiome of Pacific oysters in response to temperature, temperature stress and infection

The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge

Effects of various pollutant mixtures on immune responses of the blue mussel (Mytilus edulis) collected at a salinity gradient in Danish coastal waters

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