Differential Kinetics of Intracellular Caspase-1-like and Caspase-3-like Enzyme Activity in Human Alloreactive CD4+ and CD8+ T Cells Undergoing Apoptosis

Ruiz, Phillip; Coleman, Larry; Wang, Fang; Enten, Jay F

doi:10.1006/clim.2001.4981

Cited by 2 publications

(3 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Caspases are a group of proteases that are generally associated with apoptosis. Activation of several caspases including caspase-1, -3, -8, and -9 has been reported to induce T-cell apoptosis (MacFarlane et al, 2000; Morley et al, 2000;Ruiz et al, 2001;Takahashi et al, 2001;Varghese et al, 2001). Our data showed that several caspases (caspase-1, -3, -4, -7, -8, -9) were down-regulated in both Jurkat and PB T cells after 24 hours of activation (Table 5).…”

Section: Lin Et Alsupporting

confidence: 59%

See 1 more Smart Citation

Comparative Microarray Analysis of Gene Expression During Activation of Human Peripheral Blood T Cells and Leukemic Jurkat T Cells

Lin

Fillmore

et al. 2003

Laboratory Investigation

View full text Add to dashboard Cite

SUMMARY: Activation of T cells involves a complex cascade of signal transduction pathways linking T-cell receptorengagement at the cell membrane to the transcription of multiple genes within the nucleus. The T-cell leukemia-derived cell line Jurkat has generally been used as a model system for the activation of T cells. However, genome-wide comprehensive studies investigating the activation status, and thus the appropriateness, of this cell line for this purpose have not been performed. We sought to compare the transcriptional profiles of phenotypically purified human CD2 ϩ T cells with those of Jurkat T cells during T-cell activation, using cDNA microarrays containing 6912 genes. About 300 genes were up-regulated by more than 2-fold during activation of both peripheral blood (PB) T cells and Jurkat T cells. The number of down-regulated genes was significantly lower than that of up-regulated genes. Only 79 genes in PB T cells and 37 genes in Jurkat T cells were down-regulated by more than 2-fold during activation. Comparison of gene expression during activation of Jurkat and PB T cells revealed a common set of genes that were up-regulated, such as Rho GTPase-activating protein 1, SKP2, CDC25A, T-cell specific transcription factor 7, cytoskeletal proteins, and signaling molecules. Genes that were commonly down-regulated in both PB T cells and Jurkat T cells included CDK inhibitors (p16, p19, p27), proapoptotic caspases, and the transcription factors c-fos and jun-B. After activation, 71 genes in PB T cells and only 3 genes in Jurkat T cells were up-regulated 4-fold or more. Of these up-regulated genes and expressed sequence tags, 44 were constitutively expressed at high levels in nonactivated Jurkat cells. Quantitative real-time RT-PCR analysis confirmed our microarray data. Our findings indicate that although there is significant overlap in the activation-associated transcriptional profiles in PB T cells compared with Jurkat T cells, there is a subset of genes showing differential expression patterns during the activation of the two cell types. (Lab Invest 2003, 83:765-776).

show abstract

Section: Lin Et Alsupporting

confidence: 59%

“…This is consistent with a previous report demonstrating that the activation of caspase 1 and 3 in CD4 ϩ and CD8 ϩ T cells was time dependent. The maximum activities of caspase-1 and -3 were observed after the proliferative period, at Days 7 and 10 (Ruiz et al, 2001). …”

Section: Lin Et Almentioning

confidence: 92%

Comparative Microarray Analysis of Gene Expression During Activation of Human Peripheral Blood T Cells and Leukemic Jurkat T Cells

Lin

Fillmore

et al. 2003

Laboratory Investigation

View full text Add to dashboard Cite

show abstract

“…It was also shown that RICD occurred through the action of multiple biochemical pathways, including both death receptor signals (FAS-FASL) and pro-apoptotic Bcl-2 family proteins targeting the mitochondria, such as Bim (24-26). As the culture time increased, these cell membrane molecules also changed (27,28), thereby causing alterations in cell response properties (29,30). Therefore, different activation states of lymphocytes were likely to be the main reason causing different results when the PLT and the present ALSA test were compared.…”

Section: Discussionmentioning

confidence: 99%

A novel noninvasive method to detect rejection after heart transplantation

Xie

et al. 2012

Braz J Med Biol Res

View full text Add to dashboard Cite

Prompt and accurate detection of rejection prior to pathological changes after organ transplantation is vital for monitoring rejections. Although biopsy remains the current gold standard for rejection diagnosis, it is an invasive method and cannot be repeated daily. Thus, noninvasive monitoring methods are needed. In this study, by introducing an IL-2 neutralizing monoclonal antibody (IL-2 N-mAb) and immunosuppressants into the culture with the presence of specific stimulators and activated lymphocytes, an activated lymphocyte-specific assay (ALSA) system was established to detect the specific activated lymphocytes. This assay demonstrated that the suppression in the ALSA test was closely related to the existence of specific activated lymphocytes. The ALSA test was applied to 47 heart graft recipients and the proliferation of activated lymphocytes from all rejection recipients proven by endomyocardial biopsies was found to be inhibited by spleen cells from the corresponding donors, suggesting that this suppression could reflect the existence of activated lymphocytes against donor antigens, and thus the rejection of a heart graft. The sensitivity of the ALSA test in these 47 heart graft recipients was 100%; however, the specificity was only 37.5%. It was also demonstrated that IL-2 N-mAb was indispensible, and the proper culture time courses and concentrations of stimulators were essential for the ALSA test. This preliminary study with 47 grafts revealed that the ALSA test was a promising noninvasive tool, which could be used in vitro to assist with the diagnosis of rejection post-heart transplantation.

show abstract

Differential Kinetics of Intracellular Caspase-1-like and Caspase-3-like Enzyme Activity in Human Alloreactive CD4+ and CD8+ T Cells Undergoing Apoptosis

Cited by 2 publications

References 13 publications

Comparative Microarray Analysis of Gene Expression During Activation of Human Peripheral Blood T Cells and Leukemic Jurkat T Cells

Comparative Microarray Analysis of Gene Expression During Activation of Human Peripheral Blood T Cells and Leukemic Jurkat T Cells

A novel noninvasive method to detect rejection after heart transplantation

Contact Info

Product

Resources

About