Differential Localization of Class III β-Tubulin Isotype and Calbindin-D28k Defines Distinct Neuronal Types in the Developing Human Cerebellar Cortex

Katsetos, Christos D.; Frankfurter, Anthony; Christakos, Sylvia; Mancall, Elliott L.; Vlachos, I; Urich, H.

doi:10.1097/00005072-199311000-00013

Cited by 80 publications

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“…Class I (Constitutive; Human h␤1) ␤I EEEEDFGEEAEEEA Class II ('Major brain' or 'major neuronal') ␤II DEQGEFEEEEGEDEA Class III ('Minor brain' or 'minor neuronal'; Human h␤4) ␤III EEEGEMYEDDDEESEAQGPK Class IVa ('Brain specific'; Human ␤5) ␤IVa EEGEFEEEAEEEVA Class IVb ('Major testis'; Human h␤2) ␤IVb EEEGEFEEEAEEEVA Class V ('Avian specific'; Chick c␤5) ␤V NDGEEAFEDEDEEEEINE Class VI ('Hematopoietic') ␤VI GLEDSEEDAEEAEVEAEDKDII al., 1982;Dietrich et al, 1987;Burgoyne et al, 1988;Caccamo et al, 1989a;Moody et al, 1989;Lee et al, 1990a,b;Easter et al, 1993;Katsetos et al, 1993;Ludueña, 1998;Farina et al, 1999;Modig et al, 1999]. However, ␤III protein is not detectable in the developing or mature Xenopus nervous system.…”

Section: Table I ␤-Tubulin Isotypesmentioning

confidence: 99%

“…During mammalian and avian development, ␤III is considered to be one of the earliest neuron-associated cytoskeletal marker proteins [Moody et al, 1989;Lee et al, 1990b;Easter et al, 1993;Katsetos et al, 1993]. Its expression, either immediately before, or during terminal mitosis, in all but cerebellar Purkinje neurons [Moody et al, 1989;Easter et al, 1993;Katsetos et al, 1993;Haendel et al, 1996], suggests that ␤III-tubulin may be regulated by transcription factors necessary for neuronal lineage commitment and early morphological differentiation [Dennis et al, 2002].…”

Section: Cellular Distribution Of Class III ␤-Tubulin Isotype In the mentioning

confidence: 99%

“…Its expression, either immediately before, or during terminal mitosis, in all but cerebellar Purkinje neurons [Moody et al, 1989;Easter et al, 1993;Katsetos et al, 1993;Haendel et al, 1996], suggests that ␤III-tubulin may be regulated by transcription factors necessary for neuronal lineage commitment and early morphological differentiation [Dennis et al, 2002].…”

Section: Cellular Distribution Of Class III ␤-Tubulin Isotype In the mentioning

confidence: 99%

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Class III β‐tubulin in human development and cancer

Katsetos

Herman²,

Mörk

2003

Cell Motil. Cytoskeleton

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The differential cellular expression of class III ␤-tubulin isotype (␤III) is reviewed in the context of human embryological development and neoplasia. As compared to somatic organs and tissues, ␤III is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of ␤III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal-and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. In adult tissues, the distribution of ␤III is almost exclusively neuron-specific. Altered patterns of expression are noted in cancer. In "embryonal"-and "adult-type" neuronal tumors of the CNS and PNS, ␤III is associated with neuronal differen- Abbreviations: bHLH, basic helix-loop-helix; ␤III, class III ␤-tubulin isotype, CNE , central nervous system enhancer; CNS, central nervous system; EGL, external granule layer; MAP, microtubule-associated protein; NCAM, neural cell adhesion molecule; OPC, oligodendrocyte precursor cell; PCNA, proliferating cell nuclear antigen; PNS, peripheral nervous system; pRb, retinoblastoma tumor suppressor protein; PSA, polysialated; SVZ, subventricular zone; WHO, World Health Organization. tiation and decreased cell proliferation. In contrast, the presence of ␤III in gliomas and lung cancer is associated with an ascending histological grade of malignancy. Thus, ␤III expression in neuronal tumors is differentiation-dependent, while in non-neuronal tumors it is aberrant and/or represents "dedifferentiation" associated with the acquisition of progenitor-like phenotypic properties. Increased expression in various epithelial cancer cell lines is associated with chemoresistance to taxanes. Because ␤III is present in subpopulations of neoplastic, but not in normal differentiated glial or somatic epithelial cells, the elucidation of mechanisms responsible for the altered expression of this isotype may provide insights into the role of the microtubule cytoskeleton in tumorigenesis and tumor progression. Cell Motil. Cytoskeleton 55: 77-96, 2003.

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Section: Table I ␤-Tubulin Isotypesmentioning

confidence: 99%

Section: Cellular Distribution Of Class III ␤-Tubulin Isotype In the mentioning

confidence: 99%

Section: Cellular Distribution Of Class III ␤-Tubulin Isotype In the mentioning

confidence: 99%

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Class III β‐tubulin in human development and cancer

Katsetos

Herman²,

Mörk

2003

Cell Motil. Cytoskeleton

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188

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“…Localization of class III β-tubulin antibody used in this study has been previously demonstrated in human and nonhuman primate brain (Katsetos et al, 1993;Kornack and Rakic, 1999;Weickert et al, 2000), as well as in brains of lower species (Easter et al, 1993). Sections processed for class III β-tubulin were first thoroughly rinsed in 0.1 M PB-T (pH 7.4) overnight.…”

Section: Immunocytochemistrymentioning

confidence: 99%

Bcl-2 immunoreactive neurons are differentially distributed in subregions of the amygdala and hippocampus of the adult macaque

Fudge

2004

Neuroscience

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The amygdala and hippocampus are key limbic structures of the temporal lobe, and are implicated in the pathology of mood disorders. Bcl-2, an intracellular protein, has recently been identified in the primate amygdala and hippocampus, and is now recognized as an intracellular target of mood stabilizing drugs. However, there are few data on the cellular phenotypes of bcl-2-expressing cells, or their distribution in specific subregions of the amygdala and hippocampus. We used a number of histochemical markers to define specific subregions of the primate amygdala and hippocampus, and examined phenotype-specific distributions of bcl-2 immunore-active cells within each subregion. Immature-appearing bcl-2 labeled neurons, which co-contain class III β-tubulin immuno-reactivity, are found in distinct subregions in each structure. In the amygdala, bcl-2 positive neurons with an immature morphology are densely distributed in the paralaminar nucleus and intercalated cell islands, the parvicellular basal nucleus, and the ventral periamygdaloid cortex and amygdalohippocampal area. In the hippocampus, immature-appearing bcl-2-labeled cells are confined to the polymorph layer (subgranular zone), and base of the granule cell layer in the dentate gyrus. Well-differentiated neurons also express bcl-2. In the amygdala, labeled cells with mature phenotypes are concentrated in the parvicellular basal nucleus, the accessory basal nucleus, and the periamygdaloid cortex. The medial nucleus and central extended amygdala also contain many well-differentiated bcl-2 positive cells. In the hippocampus, the dentate gyrus and Ammon's horn contain many bcl-2 immunoreactive nonpyramidal cells. These are preferentially distributed in the rostral hippocampus. CA3 and CA2 contain relatively higher concentrations of bcl-2-labeled cells than CA1 and the subiculum. Bcl-2 is thus important in intrinsic circuitry of the hippocampus, and in amygdaloid subregions modulated by the hippocampus. In addition, the extended amygdala, a key amygdaloid output, is richly endowed with bcl-2 positive cells. This distribution suggests a role for bcl-2 in circuits mediating emotional learning and memory which may be targets of mood stabilizing drugs.Keywords paralaminar nucleus; intercalated islands; dentate gyrus; class III β-tubulin; extended amygdala; mood disorderThe amygdala is involved in higher emotional processing (Ledoux, 1992;Meunier et al., 1999;Nishijo et al., 1988;Parkinson et al., 2001), and is modulated by the hippocampus which conveys sensory information associated with past emotional experiences (Mishkin, 1982; *Correspondence to: J. L. Fudge, Department of Psychiatry, University of Rochester Medical Center, Rochester, NY 14642, USA. Tel: +1-585-273-2028; fax: +1-585-756-5334. E-mail address: julie_fudge@urmc.rochester.edu (J. L. Fudge). NIH Public Access Author ManuscriptNeuroscience. Author manuscript; available in PMC 2008 June 21. Published in final edited form as:Neuroscience. 2004 ; 127(2): 539-556. NIH-PA Author ManuscriptNIH-PA Aut...

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“…Some studies have associated expression of Class III b-tubulin with histologically high grade of malignancy in non-neuronal tumors (Katsetos et al 2003a), and recent studies have described Class III btubulin as a marker of cancer cell resistance to taxanes (Hasegawa et al 2003;Ferlini et al 2005;Lee et al 2007). Expression of Class III b-tubulin has also been shown to represent a useful tool to study early phases of neuronal differentiation in human embryonic development (Easter et al 1993;Katsetos et al 1993Katsetos et al ,2003bKukharskyy et al 2004). The expression of Class III b-tubulin in mitotic spindles of neuronal precursors has been documented (Memberg and Hall 1995).…”

Section: Introductionmentioning

confidence: 99%

Class III β-Tubulin Is a Component of the Mitotic Spindle in Multiple Cell Types

Jouhilahti

Peltonen

2008

J Histochem Cytochem.

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S U M M A R Y The findings of this study show that Class III b-tubulin is a component of the mitotic spindle in multiple cell types. Class III b-tubulin has been widely used as a neuronspecific marker, but it has been detected also in association with breast and pancreatic cancers. In this study, we describe a novel finding of Class III b-tubulin in a subpopulation of cells in malignant peripheral nerve sheath tumor. The findings of this study also show that Class III b-tubulin is expressed by normal mesenchymal and epithelial cells (fibroblasts and keratinocytes), two transitional cell carcinoma cell lines, and neurofibroma Schwann cells, as shown by immunolabeling and Western transfer analysis using two different Tuj-1 antibodies that are specific for Class III b-tubulin. The corresponding mRNA was detected using RT-PCR and whole human genome microarrays. Both antibodies localized Class III b-tubulin to the mitotic spindle and showed a colocalization with a-tubulin. The immunoreaction became visible in early prophase, and the most intense immunoreaction was detected during metaphase and anaphase when microtubules were connected to the kinetochores on chromosomes. Class III b-tubulin-specific immunoreaction lasted to the point when the midbody of cytokinesis became detectable.

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Differential Localization of Class III β-Tubulin Isotype and Calbindin-D28k Defines Distinct Neuronal Types in the Developing Human Cerebellar Cortex

Cited by 80 publications

References 0 publications

Class III β‐tubulin in human development and cancer

Class III β‐tubulin in human development and cancer

Bcl-2 immunoreactive neurons are differentially distributed in subregions of the amygdala and hippocampus of the adult macaque

Class III β-Tubulin Is a Component of the Mitotic Spindle in Multiple Cell Types

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