Differential mechanisms regarding triclosan vs. bisphenol A and fluorene-9-bisphenol induced zebrafish lipid-metabolism disorders by RNA-Seq

Sun, Liankun; Ling, Yuhang; Jiang, Jiahui; Wang, Danting; Wang, Junxia; Li, Jieyi; Wang, Xuedong; Wang, Huili

doi:10.1016/j.chemosphere.2020.126318

Cited by 107 publications

(43 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Exposure concentrations for the eight EDCs were based on their respective LC 50 and EC 50 values for embryonic and adult zebrafish (Oliveira et al, 2009;Moreman et al, 2017), environmentally relevant concentrations, and our preliminary experimental results. Exposure levels for TCS, BPA and BHPF were set at 200 μg/L, which fell between 33 and 50% of their respective LC 50 values (261-420 μg/L) (Oliveira et al, 2009;Moreman et al, 2017;Lin et al, 2017;Zhang et al, 2018;Sun et al, 2020). Similarly, an exposure concentration of 125 μg/L was selected for POP based on ca.…”

Section: Zebrafish Maintenance and Selection Criteria For Edc-exposurmentioning

confidence: 99%

“…The cycle process followed 40 cycles at 95 � C for 5 min, 95 � C for 15 s and 60 � C for 1 min. Quantification adopted the 2 -ΔΔCt method as described by Sun et al (2020). All PCR reactions included three biological replicates and each biological replicate included three technological replicates.…”

Section: Qrt-pcr Analysismentioning

confidence: 99%

“…We extracted zebrafish genomic DNA using the EasyPure Genome DNA Kit (Transgene, Shenzhen, China) according to manufacturer's protocols. PCR operations strictly followed our previous report (Sun et al, 2020). Validated plasmids containing target fragments were gel-purified and ligated to pEASY-Blunt Simple Cloning Vector (Transgene).…”

Section: Construction Of Estrogen-receptor Reporter Plasmid and Lucifmentioning

confidence: 99%

“…Lin et al (2017) reported that TCS acted on GPER, but not conventional receptors (ERα and ERβ). Additionally, high throughput RNA-seq identified differentially expressed genes induced by TCS or BPA exposure varied greatly because of their different estrogenic target molecules (Sun et al, 2020). For example, BPA inhibited lipid oxidation, while TCS mainly disrupted lipid synthesis and transport at the transcription level in larval zebrafish.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Identification of receptors for eight endocrine disrupting chemicals and their underlying mechanisms using zebrafish as a model organism

Huang

Lin

et al. 2020

Ecotoxicology and Environmental Safety

Self Cite

View full text Add to dashboard Cite

Herein, eight common endocrine disrupting chemicals (EDCs) were exposed to zebrafish (Danio rerio) to investigate the relationship between different EDCs and their activated estrogen receptors. Under acute exposure, we identified five major malformation types whose incidence and deformity modes differed among EDCs. Luciferase analysis divided the EDC receptors into four categories: (i) triclosan (TCS), 17ß-estradiol (E2) and estriol (E3) mainly activated GPER expression; (ii) bisphenol A (BPA), p-(tert-octyl) phenol (POP), 17α-ethynylestradiol (EE2), E2 and E3 activated ERβ expression; (iii) E2 and E3 acted on both GPER and ERβ; and (iv) estrone (E1) and 9,9-bis(4-hydroxyphenyl)fluorene (BHPF) had little effect on the two receptors. In vivo immunofluorescence experiments on 96-hpf larvae provided evidence that TCS and POP acted on GPER and ERβ, respectively, while E2 acted on the two receptors simultaneously. Luciferase activities in the promoter regions of gper (À 986 to À 488) and erβ (À 1998 to À 1496) were higher than those in other regions, identifying these key regions as targets for transcription activity. TCS promoted GPER expression by acting on the JUND transcription factor, while POP promoted ERβ expression by activating the Foxl1 transcription factor. In contrast, E2 mainly regulated transcription of GPER and ERβ by Arid3a. These findings provide compelling evidence that different EDCs possess varying estrogen receptors, leading to differential regulatory pathways and abnormality symptoms. These results offer an experimental strategy and fundamental information to assess the molecular mechanisms of EDC-induced estrogen effects.

show abstract

Section: Zebrafish Maintenance and Selection Criteria For Edc-exposurmentioning

confidence: 99%

Section: Qrt-pcr Analysismentioning

confidence: 99%

Section: Construction Of Estrogen-receptor Reporter Plasmid and Lucifmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of receptors for eight endocrine disrupting chemicals and their underlying mechanisms using zebrafish as a model organism

Huang

Lin

et al. 2020

Ecotoxicology and Environmental Safety

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, bisphenol A displays potential cardiotoxic and embryotoxic impacts with reduced cell viability and myocardial differentiation as well as combined effects (synergistic and additive) under multiresidue interaction 15 . Also, bisphenol A and triclosan exposure (acute and chronic) caused metabolic alterations, i.e., increased lipid accumulation and impaired lipid metabolism (e.g., disrupted lipid synthesis, transport, and degradation) in zebrafish (Danio rerio); moreover, triclosan triggered most severe hepatic steatosis 16 . Ecological and human exposure to bisphenol A are of great concern especially given bisphenol A showed the third-highest mean concentration in both the river water (before treatment) and tap water (after treatment), following caffeine and diclofenac, which ranked first and second, respectively (Fig.…”

Section: Resultsmentioning

confidence: 99%

Occurrence of multiclass endocrine disrupting compounds in a drinking water supply system and associated risks

Wee

Aris

Yusoff

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Contamination by endocrine disrupting compounds (EDCs) concerns the security and sustainability of a drinking water supply system and human exposure via water consumption. This study analyzed the selected EDCs in source (river water, n = 10) and supply (tap water, n = 155) points and the associated risks. A total of 14 multiclass EDCs was detected in the drinking water supply system in Malaysia. Triclosan (an antimicrobial agent) and 4-octylphenol (a plasticizer) were only detected in the tap water (up to 9.74 and 0.44 ng/L, respectively). Meanwhile, chloramphenicol and 4-nonylphenol in the system were below the method detection limits. Bisphenol A was observed to be highest in tap water at 66.40 ng/L (detection: 100%; median concentration: 0.28 ng/L). There was a significant difference in triclosan contamination between the river and tap water (p < 0.001). Overall, the life groups were estimated at no possible risk of EDCs (risk quotient < 1). Nonetheless, the results concern the transport and impact of EDCs on the drinking water supply system regarding treatment sustainability and water security. Further exploration of smart monitoring and management using Big Data and Internet of Things and the need to invent rapid, robust, sensitive, and efficient sensors is warranted.

show abstract

Establishment of a non‐alcoholic fatty liver disease model by high fat diet in adult zebrafish

Zhou

Zheng

et al. 2023

Anim Models and Exp Med

View full text Add to dashboard Cite

BackgroundNon‐alcoholic fatty liver disease (NAFLD) has become the most common chronic liver disease in recent years, but the pathogenesis is not fully understood. Therefore, it is important to establish an effective animal model for studying NAFLD.MethodsAdult zebrafish were fed a normal diet or a high‐fat diet combined with egg yolk powder for 30 days. Body mass index (BMI) was measured to determine overall obesity. Serum lipids were measured using triglyceride (TG) and total cholesterol (TC) kits. Liver lipid deposition was detected by Oil Red O staining. Liver injury was assessed by measuring glutathione aminotransferase (AST) and glutamic acid aminotransferase (ALT) levels. Reactive oxygen species (ROS) and malondialdehyde (MDA) were used to evaluate oxidative damage. The level of inflammation was assessed by qRT‐PCR for pro‐inflammatory factors. H&E staining was used for pathological histology. Caspase‐3 immunofluorescence measured apoptosis. Physiological disruption was assessed via RNA‐seq analysis of genes at the transcriptional level and validated by qRT‐PCR.ResultsThe high‐fat diet led to significant obesity in zebrafish, with elevated BMI, hepatic TC, and TG. Severe lipid deposition in the liver was observed by ORO and H&E staining, accompanied by massive steatosis and ballooning. Serum AST and ALT levels were elevated, and significant liver damage was observed. The antioxidant system in the body was severely imbalanced. Hepatocytes showed massive apoptosis. RNA‐seq results indicated that several physiological processes, including endoplasmic reticulum stress, and glucolipid metabolism, were disrupted.ConclusionAdditional feeding of egg yolk powder to adult zebrafish for 30 consecutive days can mimic the pathology of human nonalcoholic fatty liver disease.

show abstract

Differential mechanisms regarding triclosan vs. bisphenol A and fluorene-9-bisphenol induced zebrafish lipid-metabolism disorders by RNA-Seq

Cited by 107 publications

References 50 publications

Identification of receptors for eight endocrine disrupting chemicals and their underlying mechanisms using zebrafish as a model organism

Identification of receptors for eight endocrine disrupting chemicals and their underlying mechanisms using zebrafish as a model organism

Occurrence of multiclass endocrine disrupting compounds in a drinking water supply system and associated risks

Establishment of a non‐alcoholic fatty liver disease model by high fat diet in adult zebrafish

Contact Info

Product

Resources

About