Differential Metabolite Accumulation in Different Tissues of Gleditsia sinensis under Water Stress and Rehydration Conditions

Abstract

Gleditsia sinensis Lam. is a woody species that can tolerate various drought conditions and has been widely used in all aspects of life, including medicine, food, cleaning products, and landscaping. However, few reports have focused on the regulatory mechanism of the drought response in G. sinensis. To understand the metabolic basis of the Gleditsia sinensis drought response, different tissues were subjected to a rehydration/dehydration treatment and subsequently analyzed using untargeted and targeted metabolomics profiling depending on gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-tandem mass (LC-MS) analytical platforms, respectively. Eight sugars, twelve amino acids, and twenty phenolic compounds were characterized. Metabolites showing a significant increase or decrease under drought stress were considered to be the key metabolites of interest for a better understanding of the drought tolerance mechanisms. The GC-MS-identified compounds were shown to undergo tissue-specific regulation in response to drought stress. Moreover, the C6C3C6 and C6C3 structures were identified by LC-MS as phenolic metabolites, which revealed their drought-response association. Significant physiological parameters were measured, including overall plant development, and the results showed that antioxidant systems could not be completely restored, but photosynthetic parameters could be recovered. The results of this research provide insight into biochemical component information mechanism of drought resistance in G. sinensis.

Ionomic and Metabolomic Analyses Reveal Different Response Mechanisms to Saline–Alkali Stress Between Suaeda salsa Community and Puccinellia tenuiflora Community

“…Phenolic compounds play primary roles in the regulation of plant’s life cycle and growth as well as the acceptance of the environmental challenge. They are the most widely distributed metabolites that are involved in environment responses ( Liu et al, 2016 ; Liu J. et al, 2020 ). Phenolic compounds are referred to as cyclic compounds containing at least one hydroxyl group attached to an aromatic ring and can be divided into three major groups, i.e., C6C1-compounds, C6C3-compounds, and C6C3C6-compounds, according to the number and binding position of convertible hydroxyl groups on the aromatic chain.…”

Comparison of Seasonally Adaptive Metabolic Response Strategies of Two Acer Species

“…Next, 400 µL of methoxylamine hydrochloride-pyridine solution (15 mg/mL) was added, vortexed uniformly, and oxim the reaction for 90 min at 37 • C. Then, 400 µL of BSTFA (with 1% TMCS) derivatization reagent and 60 µL of hexane were added, vortexed uniformly, and reacted at 70 • C for 60 min. The extracts were filtered through 0.22 µm membrane and then analyzed by GC-MS [44].…”