Differential methylation of circRNA m6A in an APP/PS1 Alzheimer's disease mouse model

Abstract

Alzheimer's disease (AD) is a chronic neurological disease characterized by memory loss and progressive cognitive impairment. The characteristic AD pathologies include extracellular senile plaques formed by β-amyloid protein deposition, neurofibrillary tangles formed by hyper-phosphorylation of τ protein and neuronal loss caused by glial cell proliferation. However, the pathogenesis of AD is still unclear. Dysregulation of RNA methylation is associated with biological processes, including neurodevelopment and neurodegenerative disease. N6-methyladenosine (m6A) is the main modification in eukaryotic RNA and may be associated with the pathophysiology of AD. Circular RNA (circRNA) is a new type of evolutionarily conserved non-coding RNA without 5'-cap and 3'-polyadenylic acid tail. circRNA undergoes m6A RNA methylation and may be involved in the pathogenesis of AD. In the present study, high-throughput sequencing was performed to assess the degree of circRNA m6A methylation in APP/PS1 AD and C57BL/6 mice. These results suggested that circRNA m6A methylation in AD mice was markedly altered compared to the control group. Furthermore, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis was used to predict associated pathways; genes with different circRNA m6A methylation in AD mice were associated with 'axon guidance', 'long-term potentiation', 'glutamatergic synapse', 'cholinergic synapse', 'GABAergic synapse' and 'long-term depression'. Methylated RNA immunoprecipitation reverse transcription-quantitative PCR demonstrated that among the eight selected circRNA m6A genes, there were five genes that demonstrated significantly increased methylation and three demonstrated significantly decreased methylation.In summary, the present study indicated that circRNA m6A methylation may be associated with pathogenesis of AD.

β-amyloid accumulation enhances microtubule associated protein tau pathology in an APPNL-G-F/MAPTP301S mouse model of Alzheimer’s disease

“…Previously we showed that interaction of MAPT with HNRNPA2B1 and m 6 A RNA mediates the progression of tauopathy ( Jiang L. et al, 2021 ). Other studies of AD indicate that m 6 A dysregulation often occurs in the context of altered expression of m 6 A writers and readers ( Han et al, 2020 ; Huang et al, 2020 ; Deng et al, 2021 ; Zhao et al, 2021 ; Zhang et al, 2023 ). Hence, we examined levels of m 6 A writers and readers in the APP NL-G-F /MAPT P301S mouse model.…”

Accumulation of m6A exhibits stronger correlation with MAPT than β-amyloid pathology in an APPNL-G-F/MAPTP301Smouse model of Alzheimer’s disease

“…Previously we showed that interaction of MAPT with HNRNPA2B1 and m 6 A RNA mediates the progression of tauopathy [23]. Other studies of AD indicate that m 6 A dysregulation often occurs in the context of altered expression of m6A writers and readers [44][45][46][47][48].…”

Emerging Roles and Mechanisms of RNA Modifications in Neurodegenerative Diseases and Glioma

“…This result was consistent with other groups’ studies where notable reductions in both neuronal m6A methylation and METTL3 expression have been observed in human AD brains compared to those without the condition, as demonstrated by immunoblot analysis [ 75 ]. For circRNA, high-throughput sequencing has revealed significant changes in circRNA m6A methylation in APP/PS1 AD mice compared to control groups [ 76 ]. This result was consistent with another group’s study where METTL3-dependent m6A-modified circular RNA, circRIMS2, was significantly upregulated in APP/PS1 AD mice, which mediated synaptic and memory impairments by activating the ubiquitination of the GluN2B subunit of the NMDA receptor [ 77 ].…”