Differential micro ribonucleic acid expression profiling in ovarian endometrioma with leuprolide acetate treatment

Kiba, Atsushi; Banno, Kouji; Yanokura, Megumi; Asada, Megumi; Nakayama, Yusuke; Aoki, Daisuke; Watanabe, Tatsuya

doi:10.1111/jog.13137

Cited by 8 publications

(6 citation statements)

References 62 publications

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“…All animals were housed in the SPF environment and got unlimited water and food. 5.0×10 6 Hep-2 cells were injected into the lift flank leg of each nude mouse. They were randomly divided into two groups with treatment of 1) shRNA-NC, and 2) TrkB-shRNA1.…”

Section: The Xenograft Tumor Model Assaymentioning

confidence: 99%

“…5 The differential expression of miRNAs may be related to the early onset and development of laryngeal carcinoma. 6 Interestingly, Several miRNAs have been reported to be associated with perturbation of the BDNF/TrkB pathway. 7 TrkB is a 145-kDa receptor tyrosine kinase which can be activated by brain-derived neurotrophic factor (BDNF) and neurotrophin 4 (NT4).…”

Section: Introductionmentioning

confidence: 99%

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<p>The Down-Regulation of TrkB Alleviates the Malignant Biological Behavior and Cancer Stem-Like Property of Laryngeal Cancer</p>

Zou

Liu

et al. 2020

CMAR

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Background: This study aimed to evaluate the effect of TrkB down-regulation on the malignant biological behavior and stem-like characteristics of laryngeal cancer. Methods: The relationship was analyzed between TrkB and clinicopathological parameters in patients with laryngeal cancer. The mRNA expressive levels of TrkB and miR-10a-5p were detected by qRT-PCR in laryngeal cancer tissues and cell lines. In vitro, Hep-2 and AMC-HN-8 cell proliferation, apoptosis and stem-like properties were detected by colony formation assay, flow cytometry, sphere formation, and Western blot, respectively. In vivo, the BALB/c nude mice model was used to evaluate the effect of TrkB on tumor growth. Results: The results showed that TrkB was related to smoking history, clinical stage, and lymph node metastasis, but had nothing to do with the gender, age, and tumor location of patients with laryngeal cancer. TrkB was highly expressed and miR-10a-5p was lowly expressed in laryngeal cancer tissues and cell lines. Down-regulation of TrkB inhibited Hep-2 and AMC-HN-8 cell proliferation and sphere formation as well as enhanced apoptosis, The result showed that miR-10a-5p bound to the 3ʹ-UTR of BDNF by a dual-luciferase reporter assay. Down-regulation of miR-10a-5p induced up-regulation of TrkB promoting development of laryngeal cancer. In vivo, down-regulation of TrkB suppressed tumor growth and inhibited the expression of stem-like marker proteins and promoted apoptosis. Conclusion: In conclusion, down-regulation of TrkB plays an important role in laryngeal cancer and is a promising target for future intervention strategies.

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Section: The Xenograft Tumor Model Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

<p>The Down-Regulation of TrkB Alleviates the Malignant Biological Behavior and Cancer Stem-Like Property of Laryngeal Cancer</p>

Zou

Liu

et al. 2020

CMAR

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“…Serum levels of three miRNA biomarkers (miR-150, miR-451a, and miR-3613) reverted to normal levels in baboons that underwent treatment [121]. Kiba and colleagues identified five miRNAs (miR-146a, miR-142-3p, miR-136, miR-125b-1, and miR-15b) that were upregulated in ovarian endometrioma tissue following treatment with leuprolide acetate (a GnrH analog) [116]. Therapies with different mechanisms of action will likely impact a different set of miRNAs, several of which have been identified in cell culture experiments.…”

Section: Discussionmentioning

confidence: 99%

“…Chang et al found that miR-125b directly binds to BMPR1B, a tumor suppressor of in ovarian cancer, and found single-nucleotide polymorphisms in this binding site that correlated with susceptibility to endometriosis [115]. Altered expression of miR-125b was also reported in ovarian endometriomas after leuprolide acetate (GnrH analog) treatment, suggesting its potential as a biomarker for monitoring treatment response [116].…”

Section: Mir-125bmentioning

confidence: 99%

MicroRNAs in endometriosis: biological function and emerging biomarker candidates†

Björkman

Taylor

2019

Biology of Reproduction

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MicroRNAs (miRNAs), a class of small noncoding RNA molecules, have been recognized as key post-transcriptional regulators associated with a multitude of human diseases. Global expression profiling studies have uncovered hundreds of miRNAs that are dysregulated in several diseases, and yielded many candidate biomarkers. This review will focus on miRNAs in endometriosis, a common chronic disease affecting nearly 10% of reproductive-aged women, which can cause pelvic pain, infertility, and a myriad of other symptoms. Endometriosis has delayed time to diagnosis when compared to other chronic diseases, as there is no current accurate, easily accessible, and noninvasive tool for diagnosis. Specific miRNAs have been identified as potential biomarkers for this disease in multiple studies. These and other miRNAs have been linked to target genes and functional pathways in disease-specific pathophysiology. Highlighting investigations into the roles of tissue and circulating miRNAs in endometriosis, published through June 2018, this review summarizes new connections between miRNA expression and the pathophysiology of endometriosis, including impacts on fertility. Future applications of miRNA biomarkers for precision medicine in diagnosing and managing endometriosis treatment are also discussed.

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“…Generally, clinicians provide the same treatment options for all types of endometriosis: hormonal suppression medications or surgical intervention, and thus there is no utility for signature testing specific to the type of lesion (ovarian, deep infiltrating, and peritoneal). Furthermore, is has been suggested that miRNA serum testing could be used as a biomarker to show response to hormonal therapy [11]. However clinically, response to therapy is determined by improvement of patients' subjective symptoms.…”

Section: Editorialmentioning

confidence: 99%

miRNA serum testing for endometriosis: recent headway on the path to clinical utility

Wesevich

Arıcı

2021

Gynecological Endocrinology

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Differential micro ribonucleic acid expression profiling in ovarian endometrioma with leuprolide acetate treatment

Cited by 8 publications

References 62 publications

<p>The Down-Regulation of TrkB Alleviates the Malignant Biological Behavior and Cancer Stem-Like Property of Laryngeal Cancer</p>

<p>The Down-Regulation of TrkB Alleviates the Malignant Biological Behavior and Cancer Stem-Like Property of Laryngeal Cancer</p>

MicroRNAs in endometriosis: biological function and emerging biomarker candidates†

miRNA serum testing for endometriosis: recent headway on the path to clinical utility

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