Differential modulation of oxidative stress, antioxidant defense, histomorphology, ion-regulation and growth marker gene expression in goldfish (Carassius auratus) following exposure to different dose of virgin microplastics

Romano, Nicholas; Renukdas, Nilima; Fischer, Hayden; Shrivastava, Jyotsna; Baruah, Kartik; Egnew, Nathan; Sinha, Amit Kumar

doi:10.1016/j.cbpc.2020.108862

Cited by 51 publications

(30 citation statements)

References 69 publications

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“…The other three treatments were exposed to 0.25 mg/L copper (Cu), 0.5 mg/L MPVC, and their combination (Cu‐MPVC), for 14 days. The concentration of copper was based on a previous study on common carp (Dautremepuits et al, 2004b), whereas, the concentration of MPVC was an environmentally relevant one according to previous reports (Lasee et al, 2017; Romano et al, 2020). During this period, the fish were fed based on 2% of their body weight daily.…”

Section: Methodsmentioning

confidence: 99%

“…Due to the polar nature of the molecule, MPs possess a high affinity for toxicants (pharmaceutical drugs and heavy metals), so that, involuntary/voluntary ingestion of MPs increases the entrance of the toxicant to fishes' body (Romano et al, 2020). In the fishes' body, due to change in the temperature, pH, and action of enzymes, adsorbed pollutants are released from MPs, thereby potentially causing various problems to the organism (Gaspar et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Available data suggest that there is rare information about the effects of PVC microparticles (MPVC) on fish. MPVC induces growth retardation, oxidative stress, and tissue histopathological damage in common carp, Cyprinus carpio , (Xia et al, 2020), and goldfish, Carassius auratus (Romano et al, 2020) fingerlings. Moreover, in gilthead seabream, Sparus aurata , and European sea bass, Dicentrarchus labrax , MPVC induces stress, oxidative stress, and changes different immune parameters, depending on the experimental conditions (Espinosa et al, 2018, 2017, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Histopathological damage and stress‐ and immune‐related genes' expression in the intestine of common carp, Cyprinus carpio exposed to copper and polyvinyl chloride microparticle

et al. 2021

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The present study aimed at assessing the singular and combined effects of water copper and polyvinyl chloride microplastic (MPVC) on intestinal copper accumulation, histopathological damage, and stress‐/immune‐related genes' expression in common carp, Cyprinus carpio. Four groups of fish were maintained in triplicate: control (kept in clean water), Cu (exposed to 0.25 mg/L of copper), MPVC (exposed to 0.5 mg/L of MPVC), and Cu‐MPVC (exposed to 0.25 mg/L of copper + 0.5 mg/L of MPVC). After 14‐day exposure, the fish of Cu and Cu‐MPVC treatments exhibited significantly higher intestinal copper contents, compared to the fish of control and MPVC treatments. In this regard, the Cu‐MPVC fish had significantly higher copper content than the Cu fish. Exposure to copper and/or MPVC significantly upregulated the intestinal heat shock protein 70 (hsp70), cytochrome P450 family 1 subfamily A member 1 (cyp1a1), lysozyme (lys), defensin (def), mucin 2 (muc2), and mucin 5 (muc5) expression. The highest expression of hsp70, cyp1a1, lys, and def was related to Cu‐MPVC treatment; whereas, the highest expression of muc2 and muc5 was observed in Cu and MPVC treatments. Exposure to copper and/or MPVC induced intestinal damage, which Cu‐MPVC fish exhibited the highest severity. The present study revealed that exposure to copper and/or MPVC causes intestinal histopathological damage and upregulation in stress‐ and immune‐related genes' expression. The most serious effects were observed in Cu‐MPVC treatment that might be due to additive effects of copper and MPVC and/or higher copper accumulation in this treatment.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Histopathological damage and stress‐ and immune‐related genes' expression in the intestine of common carp, Cyprinus carpio exposed to copper and polyvinyl chloride microparticle

et al. 2021

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“…The impacts of MP exposure have been extensively studied in aquatic animals (e.g. Barboza et al., 2018; De Sales‐Ribeiro et al., 2020; Karami et al., 2016; Romano et al, 2020), and sometimes with contrasting findings. This could be due to differences in MP type and concentration.…”

Section: Introductionmentioning

confidence: 99%

Microplastics affected black soldier fly (Hermetiaillucens) pupation and short chain fatty acids

Romano

Fischer

2021

J Applied Entomology

Self Cite

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Plastics are used in almost every form of daily life where it is estimated that 300 million tonnes are produced each year, with 51% of production in Asia (PlasticsEurope, 2019). Unfortunately, plastics are often single-use and thus end up in landfills and water ways where they eventually degrade into microscopic (1-1,000 µm) secondary microplastics (MPs) (Burns & Boxall, 2018). Typically, secondary MPs are non-uniformly shaped fragments and the most commonly found include polyethylene (PE), polypropylene (PP), polyvinyl chloride (PVC) and polystyrene (PS) (do Sul et al., 2013). The prevalence of MPs in various waterways, both freshwater and marine, has been globally documented, but are also being found in the air and soil (Enyoh et al., 2019; Oliveira & Almedia, 2019; Möller et al., 2020). Oliveira & Almedia (2019) emphasized that the prevalence of MPs is substantially understudied in soils, despite having a high capacity to retain and accumulate MPs. Perhaps not surprisingly, MPs in uncontrolled landfills are substantially higher and can reach up to 91 particles/g refuse (He et al., 2019; Hou et al., 2021; Su et al., 2019). According to the most recent estimates, plastics are the third most common material in landfills at 19.2%, based on weight in the United States (EPA (Environmental Protection Agency), 2020). Paper and food waste are first and second, respectively, in landfills based on weight (EPA (Environmental Protection Agency), 2020). Thus, im-properly managed landfills attract various pests and insects, including black soldier flies, Hermetia illucens (Fadila et al., 2020;Qasim et al., 2020), which makes it a real possibility that black soldier fly larvae (BSFL) can be exposed to MPs. This could have ecological implications, especially considering adult black soldier flies are pollinators (Srikanth et al., 2013). Moreover, there has been increasing interest in feeding BSFL with landfill residue and leachate as an environmentally friendly method to convert this waste into a high-value prod-

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“…Despite this, the gill tissues of larvae showed damage phenomenon. Differently from what reported above, some authors found no damage or alteration on gills after MPs and NPs exposure [133,137]. The inconsistencies in previous studies may be due to the different experimental concentrations employed (Table 2).…”

Section: Occurrence Of Micro and Nano-plastics In Fish Gills And Consequent Effectsmentioning

confidence: 57%

Micro and Nano Plastics Distribution in Fish as Model Organisms: Histopathology, Blood Response and Bioaccumulation in Different Organs

et al. 2021

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Micro- and nano-plastic (MP/NP) pollution represents a threat not only to marine organisms and ecosystems, but also a danger for humans. The effects of these small particles resulting from the fragmentation of waste of various types have been well documented in mammals, although the consequences of acute and chronic exposure are not fully known yet. In this review, we summarize the recent results related to effects of MPs/NPs in different species of fish, both saltwater and freshwater, including zebrafish, used as model organisms for the evaluation of human health risk posed by MNPs. The expectation is that discoveries made in the model will provide insight regarding the risks of plastic particle toxicity to human health, with a focus on the effect of long-term exposure at different levels of biological complexity in various tissues and organs, including the brain. The current scientific evidence shows that plastic particle toxicity depends not only on factors such as particle size, concentration, exposure time, shape, and polymer type, but also on co-factors, which make the issue extremely complex. We describe and discuss the possible entry pathways of these particles into the fish body, as well as their uptake mechanisms and bioaccumulation in different organs and the role of blood response (hematochemical and hematological parameters) as biomarkers of micro- and nano-plastic water pollution.

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Differential modulation of oxidative stress, antioxidant defense, histomorphology, ion-regulation and growth marker gene expression in goldfish (Carassius auratus) following exposure to different dose of virgin microplastics

Cited by 51 publications

References 69 publications

Histopathological damage and stress‐ and immune‐related genes' expression in the intestine of common carp, Cyprinus carpio exposed to copper and polyvinyl chloride microparticle

Histopathological damage and stress‐ and immune‐related genes' expression in the intestine of common carp, Cyprinus carpio exposed to copper and polyvinyl chloride microparticle

Microplastics affected black soldier fly (Hermetiaillucens) pupation and short chain fatty acids

Micro and Nano Plastics Distribution in Fish as Model Organisms: Histopathology, Blood Response and Bioaccumulation in Different Organs

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