Differential Modulation of the Metabolic Effects of Diet-Induced Obesity by Probiotic <i>Lactobacillus Paracasei</i> K56 and Prebiotic α-Galactooligosaccharides

Min, Qing-Qing; Sun, Ting; Xu, Jia‐Ying; Chen, Yu Zong; Liu, Wei-Hsien; Wen, Zhang; Hao, Jingyu; Zhao, Zifu; Hung, Wei-Lian; Qin, Liqiang

doi:10.37290/ctnr2641-452x.19:21-28

Cited by 6 publications

(5 citation statements)

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“…Some specific Lacticaseibacillus paracasei improved glycolipid metabolism [23,24]. Lacticaseibacillus paracasei K56 was isolated from the intestinal tract of children, improved obesity, and regulated the intestinal flora of the host with a high-fat diet [9,10]. However, the mechanism of action of K56 on lipid…”

Section: Discussionmentioning

confidence: 99%

“…It is generally recognized as safe (GRAS) [8]. K56 has demonstrated the potential to prevent obesity, effectively improving glycolipid metabolism in mice fed with a high-fat diet [9,10]. However, probiotics do not directly interact with adipose tissues in vivo, and glycolipid metabolism is a complex process involving lipogenesis and lipolysis.…”

Section: Introductionmentioning

confidence: 99%

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Lacticaseibacillus paracasei K56 inhibits lipid accumulation in adipocytes by promoting lipolysis

Wang,

Li,

Liu

et al. 2024

Food Science and Human Wellness

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Probiotics crosstalk immunity to improve host glycolipid metabolism, which is a new strategy for obesity. This study aimed to explore the functions of Lacticaseibacillus paracasei K56 (K56), in regulating the lipid metabolism of adipocytes and its immune regulation mechanisms.We co-cultured RAW264.7 macrophages with K56, and the K56-stimulated RAW264.7-conditioned medium (K56-CM) was collected and treated with 3T3-L1 pre-adipocytes. The expression of lipid metabolism-related markers of adipocytes, and the content of cytokines in CMs were detected. The results demonstrated that K56-CM promoted the expression of peroxisome proliferator-activated receptor-γ (PPAR-γ), CCAAT/enhancer binding protein α (C/EBPα), and other adipogenesis-related markers, which resulted in the upregulation of lipolysis markers, such as hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL). The activation of lipolysis enhanced the expression of fatty acid β-oxidation-related and mitochondrial biogenesis-related markers and reduced lipid accumulation in adipocytes. The glycolipid metabolism pattern of K56 may be due to its immunomodulatory characteristics, which stimulated macrophages to secrete fewer TNF-α, thereby promoting the expression of lipolysis-related markers, and TNF-α synergized with lipase to promote lipolysis. It has not been reported that Lacticaseibacillus paracasei modulated lipid metabolism via the lipolysis pathway, which suggested that K56 may regulate glycolipid metabolism of the host by maintaining immune homeostasis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lacticaseibacillus paracasei K56 inhibits lipid accumulation in adipocytes by promoting lipolysis

Wang,

Li,

Liu

et al. 2024

Food Science and Human Wellness

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“…In a previously reported animal study, high fat diet induced obese mice were treated by gavage five times a week with freshly prepared K56 (10 6 CFU/day or 10 8 CFU/day) alone or in combination with α- galactooligosaccharides for 12 weeks. After treatment, all probiotic groups significantly decreased body weight gain and visceral fat than high fat diet (HFD) group, especially at the dosage of 10 8 CUF/day alone or combined with α-GOS had lower body weight and fat gain than 10 6 CFU/day group [ 23 ]. In another animal study, HFD-fed mice were administered K56 suspension of 10 7 CFU/day, 10 9 CFU/day, and 10 11 CFU/day.…”

Section: Discussionmentioning

confidence: 99%

“…In a recently published animal study, a novel probiotic strain Lacticaseibacillus paracasei K56, isolated from the intestine of a healthy child, was treated by gavage at various doses to HFD-fed mice for 12 weeks. The results have shown that L. paracasei K56 significantly reduced body and fat mass and improved lipid metabolism [ 23 ]. In another animal study, administration of Lacticaseibacillus paracasei K56 effectively attenuated obesity parameters, such as body weight, insulin-resistance, plasma glucose and lipids; The beneficial effects may be related to the restored host gut microbiota [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of different doses of Lacticaseibacillus paracasei K56 on body fat and metabolic parameters in adult individuals with obesity: a pilot study

Kadeer

et al. 2023

Nutr Metab (Lond)

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Background Studies have shown that probiotics have an effect on reducing body fat on a strain-specific and dose–response bases. The purpose of this study was to evaluate the effect of a novel probiotic strain Lacticaseibacillus paracasei K56 on body fat and metabolic biomarkers in adult individuals with obesity. Methods 74 adult subjects with obesity (body mass index ≥ 30 kg/m2, or percent body fat > 25% for men, percent body fat > 30% for women) were randomized into 5 groups and supplemented with different doses of K56 (groups VL_K56, L_K56, H_K56, and VH_K56: K56 capsules, 2 × 107 CFU/day, 2 × 109 CFU/day, 2 × 1010 CFU/day, 2 × 1011 CFU/day, respectively) or placebo (group Pla: placebo capsule) for 60 days. Subjects were advised to maintain their original dietary intake and physical activity. Anthropometric measurements, body composition assessment, and metabolic parameters were measured at baseline and after 60 days of intervention. Results The results showed that the L_K56 group had significant decreases in percent body fat (p = 0.004), visceral fat area (p = 0.0007), total body fat mass (p = 0.018), trunk body fat mass (p = 0.003), waist circumference (p = 0.003), glycosylated hemoglobin(p = 0.002) at the end of the study compared with baseline. There were non-significant reductions in Body weight and BMI in the L_K56, H_K56, VL_K56 groups, whereas increases were observed in the placebo and VH_K56 groups compared with baseline values. In addition, K56 supplementation modulated gut microbiota characteristics and diversity indices in the L-K56 group. However, mean changes in body fat mass, visceral fat area, weight, body mass index, waist circumference and hip circumference were not significantly different between groups. Conclusions The results suggest that supplementation with different doses of Lacticaseibacillus paracasei K56 has certain effect on reducing body fat and glycosylated hemoglobin, especially at a dose of 109 CFU/day. Trial registration: clinicaltrials.gov Identifier: NCT04980599.

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“…Treatment with Lactobacillus paracasei K56 and α-galacto-oligosaccharides significantly reduced body weight and fat mass, especially when α-galacto-oligosaccharides were given with a high concentration of L. paracasei K56. In addition, both α-galacto-oligosaccharides and Lactobacillus paracasei K56 significantly modulated obesity and improved lipid metabolism (Min et al, 2020). After examining the prebiotic effects of Lactobacillus fermentum CECT5716 in high-fat diet-induced obese mice, this prebiotic was demonstrated to possess antiobesity and anti-inflammatory properties, improve endothelial dysfunction and intestinal dystrophy, restore the abundance of Akkermansia, reduce the proportions of Erysipelotrichi and Clostridium, and increase the abundance of Bacteroidetes.…”

Section: Research Status Of Lactic Acid Bacteria In the Prevention An...mentioning

confidence: 99%

Relationship between probiotics and obesity: a review of recent research

Zhang¹,

LI³

2022

Food Sci. Technol

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The percentage of obese individuals continues to grow worldwide, and the main factor leading to obesity is the imbalance of energy intake and consumption. The intestinal flora has important regulatory roles in metabolic disorders and obesity. Compared with normal-weight individuals, those that are obese showed remarkable changes in the composition of the intestinal flora. Several studies have reported that probiotics can reverse high-fat diet-induced obesity. Thus, altering the intestinal flora may be an effective approach to control or even prevent obesity, and some probiotics have been demonstrated to have effects on obesity. The purpose of this review is to summarize the experimental means used to study probiotics and obesity and to summarize the weight-loss effects and mechanisms of action of experimental and clinical probiotics, so as to help investigators understand the latest benefits of weight-loss probiotics and to provide a reference for follow-up research in this field.

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Differential Modulation of the Metabolic Effects of Diet-Induced Obesity by Probiotic Lactobacillus Paracasei K56 and Prebiotic α-Galactooligosaccharides

Cited by 6 publications

References 0 publications

Lacticaseibacillus paracasei K56 inhibits lipid accumulation in adipocytes by promoting lipolysis

Lacticaseibacillus paracasei K56 inhibits lipid accumulation in adipocytes by promoting lipolysis

Effect of different doses of Lacticaseibacillus paracasei K56 on body fat and metabolic parameters in adult individuals with obesity: a pilot study

Relationship between probiotics and obesity: a review of recent research

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