Differential morphologic and metabolic alterations in permissive versus nonpermissive murine macrophages infected with Legionella pneumophila

Yamamoto, Yoshimasa; Klein, Thomas; Brown, H. Keith; Friedman, Herman

doi:10.1128/iai.60.8.3231-3237.1992

Cited by 18 publications

(11 citation statements)

References 19 publications

(12 reference statements)

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“…The cfu data and the MTT-viability results suggest that at 24 h postinfection, BALB/c macrophages probably express some unknown mechanism that makes them able to control the intracellular proliferation of L. pneumophila, while A/J macrophages are deficient in such a system. These data support our previous observation by electron microscopic examination whereby L. pneumophila were readily detected within A/J mouse TG macrophages after in vitro infection but were not seen within cells of other mouse strains [12].…”

Section: Discussionsupporting

confidence: 92%

“…Therefore, nitric oxide metabolism might not be related to the genetic factors responsible for the differences in permissiveness between A/J and BALB/c macrophages. On the other hand, we previously reported that L. pneumophila vaccine is not capable of inducing detectable H202 and O 2 in macrophages [12], and that scavengers of HzO 2 (i.e. catalase) and O~ (superoxide dismutase) show little effect on the microbicidal activity of activated A/J macrophages to L. pneumophila [27].…”

Section: Discussionmentioning

confidence: 86%

“…catalase) and O~ (superoxide dismutase) show little effect on the microbicidal activity of activated A/J macrophages to L. pneumophila [27]. However, when macrophages were stimulated with zymosan or the non-specific membrane activator merezein, BALB/c and BDF 1 cells produced three times more HzO 2 than did the cells from the A/J mice (data not shown) [12]. This observation opens the possibility that oxygen-dependent microbicidal mechanisms might participate, at least partially, as a restriction mechanism for L. pneumophila growth in non-permissive BALB/c and BDF 1 cells.…”

Section: Discussionmentioning

confidence: 92%

“…Summersgill et al [10] found that induced nitric oxide synthase activity in RAW 264.7 cells, a murine macrophage cell line, was correlated with killing of L. pneumophila and depletion of intracellular iron. Since the mechanism explaining the differences in susceptibility among macrophages from different mouse strains remains to be determined [11,12], we studied the role of iron in macrophages from two mouse strains which differ in permissiveness to intracellular L. pneumophila growth.…”

Section: Introductionmentioning

confidence: 99%

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Differences and similarities in permissive A/J versus non-permissive BALB/c murine macrophages infected with Legionella pneumophila: The role of iron

Gebran¹

1994

FEMS Immunology and Medical Microbiology

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Legionella pneumophila, a facultative intracellular pathogen, replicates within and kills thioglycolate-elicited (TG) macrophages from A/J mice, while growth is inhibited in TG macrophages from BALB/c mice which show no impaired viability. The role of iron in BALB/c and A/J macrophages regarding their permissiveness to L. pneumophila intracellular growth was investigated. We previously reported that TG macrophages from the A/J mouse strain readily supported the intracellular growth of L. pneumophila, while resident macrophages from the same strain of mice were not permissive. Recently we also found that such a difference in permissiveness between both A/J macrophage populations may be explained, at least in part, to intracellular availability of iron. In this report, differences in permissiveness to L. pneumophila growth between A/J TG macrophages and BALB/c TG macrophages was not due to intracellular iron availability. BALB/c and A/J TG macrophages exhibited similar expression of transferrin receptor and cellular iron content. The treatment of BALB/c TG macrophages with different iron compounds, namely ferric nitrilotriacetate (12.5-100 microM), ferric citrate (12.5-100 microM) and transferrin (0.5-5 mg ml-1), did not stimulate the intracellular proliferation of L. pneumophila. The reduction of intracellular iron availability by treatment with antibodies against transferrin receptor or with desferrioxamine suppressed the growth of L. pneumophila within BALB/c TG macrophages, suggesting that these cells do not restrict L. pneumophila growth because of iron. The production of nitric oxide was also similar in both macrophage populations, as measured by the Griess reaction. However, the synthesis of oxygen reactive species was three times higher in non-permissive BALB/c macrophages.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differences and similarities in permissive A/J versus non-permissive BALB/c murine macrophages infected with Legionella pneumophila: The role of iron

Gebran¹

1994

FEMS Immunology and Medical Microbiology

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“…Electron Microscopy. Macrophage monolayers on glass coverslips were prepared as previously described (9).…”

Section: Methodsmentioning

confidence: 99%

Direct Ultrastructural Imaging of Macrophages Using a Novel X‐Ray Contact Microscopy

Kado

Richardson

Rajyaguru

et al. 1999

Proc Soc Exp Biol Med

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A compact, high-resolution, laser-plasma, x-ray contact microscopy method using a table-top Nd:glass laser system has been developed. This x-ray microscopy system was applied for the observation of macrophage ultrastructures. These images were produced using proximity imaging in which a 5-ns pulse of soft x-rays with wavelengths near and inside the water windows (23A-44A) produced by the laser-plasma were absorbed by the specimen and then registered on a photo resist. The x-ray images imprinted on the photo resist were then developed and analyzed with an atomic force microscope (AFM). Mouse thioglycollate-elicited peritoneal macrophages in suspension were examined by this new x-ray microscope. The x-ray images of the macrophages were compared with those observed by conventional transmission electron microscopy (TEM). The x-ray images showed no obvious organelles, including the nucleus and endoplasmic reticulum, as can be seen with TEM, but high- and low-contrast structures caused by mass distribution of carbon were observed. Thus, using the x-ray microscopy we visualized the first x-ray images of macrophage ultrastructures. The successful x-ray imaging of macrophage ultrastructure indicates that proximity x-ray microscopy may be of value in studying physiology linked to the dynamics of a cell.

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Cytopathology of Pathogenic Prokaryotes

Brogden

2009

Ultrastructural Pathology the Comparative Cellular Basis of Disease

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Differential morphologic and metabolic alterations in permissive versus nonpermissive murine macrophages infected with Legionella pneumophila

Cited by 18 publications

References 19 publications

Differences and similarities in permissive A/J versus non-permissive BALB/c murine macrophages infected with Legionella pneumophila: The role of iron

Differences and similarities in permissive A/J versus non-permissive BALB/c murine macrophages infected with Legionella pneumophila: The role of iron

Direct Ultrastructural Imaging of Macrophages Using a Novel X‐Ray Contact Microscopy

Cytopathology of Pathogenic Prokaryotes

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