Differential nano-bio interactions and toxicity effects of pristine versus functionalized graphene

Sasidharan, Abhilash; Panchakarla, Leela S.; Chandran, Parwathy; Menon, Deepthy; Nair, Shantikumar V.; Rao, C. N. R.; Koyakutty, Manzoor

doi:10.1039/c1nr10172b

Cited by 418 publications

(317 citation statements)

References 26 publications

Supporting

Mentioning

303

Contrasting

Unclassified

Order By: Relevance

“…[ 69,70 ] While pristine carbon based materials tend to accumulate in physiological solution due to electrostatic interactions and nonspecifi c bindings to proteins, [ 71 ] surface-modifi ed graphene-like materials are often deemed to be less toxic. [ 70,72 ] Our results are consistent with these observations. These results demonstrate that the functionalization of coronene and TRGO with D -phenylalanine NDIs considerably improves the in vitro biocompatibility of the pristine PCSs presented in this work.…”

Section: Advsupporting

confidence: 83%

Thermally Reduced Graphene Oxide Nanohybrids of Chiral Functional Naphthalenediimides for Prostate Cancer Cells Bioimaging

Tyson

Mirabello

Calatayud

et al. 2016

Adv Funct Materials

View full text Add to dashboard Cite

This study reports on the supramolecular assemblies formed between planar carbon systems (PCSs) such as thermally reduced graphene oxide (TRGO) and its small‐molecule model system coronene and a series of d‐ and l‐α amino acid derivatized naphthalenediimides (NDIs) where the halogen substituents (X = F, Cl, Br, I) are varied systematically. Confocal fluorescence microscopy of NDIs, NDI•coronene, and NDI•TRGO complexes is performed proving the uptake and stability of such complexes in the cellular environment and suggesting their potential as prostate cancer imaging agents. 1H NMR and UV–vis spectroscopy studies support the formation of charge transfer complexes whereby the increasing polarizability and general electronegativity of the aryl halide substituted at the NDI periphery influence the magnitude of the association constants in the ground state between NDI and coronene. Complexation between NDIs and PCSs also results in stable photoexcited assemblies within the solution (coronene) as well as the dispersed phased (TRGO). Fluorescence emission titrations and 2‐photon time correlated single photon counting measurements suggest the existence of dynamic quenching mechanisms upon the excitation of the fluorophore in the presence of the carbon substrates, as these methods are sensitive proves for the subtle changes in the NDI environment. The series of halogenated species used exerts supramolecular control over the degree of surface assembly on the TRGO and over the interactions with the coronene molecule, and this is of relevance to the assembly of future biosensing platforms as these materials can both be viewed as congeners of graphene. Finally, MTT assays carried out in PC‐3 cells demonstrate that the stable noncovalent functionalization of TRGO and coronene with either l or d NDIs remarkably improves the cellular viability in the presence of such graphene‐like materials. These phenomena are of particular relevance for the understanding of the direct donor–acceptor interactions in solutions which govern the design of nanomaterials with future biosensing and bioimaging applications.

show abstract

Section: Advsupporting

confidence: 83%

Thermally Reduced Graphene Oxide Nanohybrids of Chiral Functional Naphthalenediimides for Prostate Cancer Cells Bioimaging

Tyson

Mirabello

Calatayud

et al. 2016

Adv Funct Materials

View full text Add to dashboard Cite

show abstract

“…The presence of GBMs in the intracellular compartments has been observed among others by Sasidharan et al thanks to confocal microscopy 28 and Huang and co-workers via surfaceenhanced Raman spectroscopy 29 . However, the underlying mechanisms of cellular internalization of GBMs remain enigmatic and several pathways have been proposed.…”

Section: What Can Gbms Offer As Gene Delivery Platforms?mentioning

confidence: 92%

Graphene materials as 2D non-viral gene transfer vector platforms

2016

View full text Add to dashboard Cite

Advances in genomics and gene therapy could offer solutions to many diseases that remain incurable today, however one of the critical reasons halting clinical progress is due to the difficulty in designing efficient and safe delivery vectors for the appropriate genetic cargo. Safety and largescale production concerns counter-balance the high gene transfer efficiency achieved with viral vectors, while non-viral strategies have yet to become sufficiently efficient. The extraordinary physicochemical, optical and photothermal properties of graphene-based materials (GBMs) could offer two-dimensional (2D) components for the design of nucleic acid carrier systems. We discuss here such properties and their implications for the optimization of gene delivery. While the design of such vectors is still in its infancy, we provide here an exhaustive and up-to-date analysis of the studies that have explored GBMs as gene transfer vectors, focusing on the functionalization strategies followed to improve vector performance and on the biological effects attained.

show abstract

“…Some references revealed that hydrophilic GO is able to penetrate cell membranes, resulting in cytoplasmatic, perinuclear and nuclear accumulation [118][119][120]; while this behavior of GO can be inhibited by chemical or physical functionalization with polymers [120]. Furthermore, GO increases the surface hydrophilicity of the biocomposites, leading to improved biocompatibility [121].…”

Section: Biological Property Of Go-based Materialsmentioning

confidence: 99%

The Preparation of Graphene Oxide and Its Derivatives and Their Application in Bio-Tribological Systems

et al. 2014

View full text Add to dashboard Cite

Graphene oxide (GO) can be readily modified for particular applications due to the existence of abundant oxygen-containing functional groups. Graphene oxide-based materials (GOBMs), which are biocompatible and hydrophilic, have wide potential applications in biomedical engineering and biotechnology. In this review, the preparation and characterization of GO and its derivatives are discussed at first. Subsequently, the biocompatibility and tribological behavior of GOBMs are reviewed. Finally, the applications of GOBMs as lubricants in bio-tribological systems are discussed in detail.

show abstract

Differential nano-bio interactions and toxicity effects of pristine versus functionalized graphene

Cited by 418 publications

References 26 publications

Thermally Reduced Graphene Oxide Nanohybrids of Chiral Functional Naphthalenediimides for Prostate Cancer Cells Bioimaging

Thermally Reduced Graphene Oxide Nanohybrids of Chiral Functional Naphthalenediimides for Prostate Cancer Cells Bioimaging

Graphene materials as 2D non-viral gene transfer vector platforms

The Preparation of Graphene Oxide and Its Derivatives and Their Application in Bio-Tribological Systems

Contact Info

Product

Resources

About