Differential non-coding RNAs expression profiles of invasive and non-invasive pituitary adenomas

Abstract

Background Pituitary adenoma (PA) accounts for 10–15% of all intracranial neoplasms. Despite their benign nature, PA often shows invasive growth. MicroRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are a class of non-coding RNAs that play important roles in PA initiation and progression. Aim The aim of this study was to find specific profiles of miR-200a and long non-coding RNA (lncRNA) antisense non-coding RNA in the INK4 locus (ANRIL) in PA based on a comparative study using Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) analyses of tumor tissue and plasma. Methods Plasma and PA tissue samples were obtained from two groups of included patients (15 invasive and 15 non-invasive PA). In addition, plasma samples from patients with invasive PA have collected pre- and post-operation. Plasma and tissue samples subjected to qRT-PCR analyses for the expression levels of miR-200a and lncRNA ANRIL. Results The expression levels of miR-200a and lncRNA ANRIL were increased in tissue samples patients with invasive PA than in the patients with non-invasive PA. In addition, the expression levels of circulating miR-200a and lncRNA ANRIL were increased in patients with invasive PA than in patients with non-invasive PA in the pre-operation period. However, the expression level of plasma circulating miR-200a and lncRNA ANRIL was decreased in patients with invasive PA in the post-operation period. Our results depicted a miR-200a and lncRNA ANRIL expression in tissue and plasma samples in the patients with invasive PA. In addition, Receiver Operating Characteristic (ROC) curve was used to evaluate the diagnostic value of these circulating miR-200a and lncRNA ANRIL. Conclusion The expression of these tumor-associated ncRNAs has been elevated in the PAs. Therefore, miR-200a and lncRNA ANRIL represents as biomarkers for diagnosis and potential targets for novel invasive PA treatment strategies.

HOTTIP Mediated Therapy Resistance in Glioma Cells Involves Regulation of EMT-Related miR-10b

“…Interestingly, one of the other shown lncRNA, H19 also seems to affect EMT in glioma cells ( 19 ). The very indication that this H19 affects MET comes through the miRNA it targets as this miRNA is very well known to be involved in regulation of EMT ( 29 ), thus making this miRNA an attractive cancer biomarker ( 30 ). Similar to our findings reported here, H9 was found to regulate EMT marker ZEB1 ( 19 ) which thus appears to be important EMT gene regulated by lncRNAs.…”

Role of miRNAs in vascular development

“…The existence of the blood vascular system and the lymphatic vascular system and the close connection between the two are essential in higher vertebrates [ 4 ]. MicroRNAs are a class of single-stranded non-coding small RNAs with a length of 21–23 nt, which function by recognizing the 3′ untranslated region sequence of the target mRNA and binding to its target, and inhibiting the translation level of its target after transcription [ [5] , [6] , [7] , [8] , [9] , [10] ]. At present, there have been many reports on the regulation of microRNAs in vascular development.…”

MicroRNAs as potential diagnostic markers of glial brain tumors

“…To date, extensive experience has been accumulated in determining the level of microRNA expression in human brain glioma tissues using various methods [ [50] , [51] , [52] , [53] , [54] , [55] , [56] ]. But there are certain obstacles that limit the application of these methods when using miRNAs in clinical diagnostics.…”